SOLR
| _version_ |
1797365277210443776 |
| author |
Schuller, Astrid |
| author2 |
Rödel, Gerhard, Ostermann, Kai |
| author2_role |
, |
| author2_variant |
g r gr, k o ko |
| author_facet |
Schuller, Astrid, Rödel, Gerhard, Ostermann, Kai |
| author_role |
|
| author_sort |
Schuller, Astrid |
| author_variant |
a s as |
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Library A |
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sid-22-col-qucosa |
| container_reference |
Sensors (2017), 17(7), ISSN 1424-8220. DOI: 10.3390/s17071506. Artikelnr.: 1506. |
| contents |
The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity. |
| dewey-full |
620 |
| dewey-hundreds |
600 - Technology (Applied sciences) |
| dewey-ones |
620 - Engineering and allied operations |
| dewey-raw |
620 |
| dewey-search |
620 |
| dewey-sort |
3620 |
| dewey-tens |
620 - Engineering and allied operations |
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Online, Free |
| finc_class_facet |
Technik |
| fincclass_txtF_mv |
engineering-process, technology |
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ElectronicSerialComponentPart |
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Article, E-Article |
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Ebook |
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Article, E-Article |
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Article, E-Article |
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Buch |
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text-online-journal-child |
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Journal |
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Article, E-Article |
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ElectronicArticle |
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Article, E-Article |
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Article, E-Article |
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E-Article |
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not assigned |
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not assigned |
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Sensors (2017), 17(7), ISSN 1424-8220. DOI: 10.3390/s17071506. Artikelnr.: 1506. |
| id |
22-14-qucosa-230652 |
| illustrated |
Not Illustrated |
| imprint |
Basel, MDPI (Multidisciplinary Digital Publishing Institute), 2017 |
| imprint_str_mv |
Online-Ausg.: 2017 |
| institution |
DE-105, DE-Gla1, DE-Brt1, DE-D161, DE-540, DE-Pl11, DE-Rs1, DE-Bn3, DE-Zi4, DE-Zwi2, DE-D117, DE-Mh31, DE-D275, DE-Ch1, DE-15, DE-D13, DE-L242, DE-L229, DE-L328 |
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|
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|
| language |
English |
| last_indexed |
2024-04-26T03:11:41.886Z |
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schuller2017tuningthesensitivityofthepdr5promoterbaseddetectionofdiclofenacinyeastbiosensors |
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Qucosa |
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2017 |
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2017 |
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Basel |
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MDPI (Multidisciplinary Digital Publishing Institute) |
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marcfinc |
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14-qucosa-230652 |
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marcfinc |
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Zi 0001 |
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22 |
| spelling |
Schuller, Astrid, Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors, Basel MDPI (Multidisciplinary Digital Publishing Institute) 2017, Online-Ausg. 2017 Online-Ressource (Text) Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity., Diclofenac, Pdr5, Hefe, Biosensor, Technische Universität Dresden, Publikationsfonds, Yeast, Publishing Fund, Rödel, Gerhard, Ostermann, Kai, Sensors (2017), 17(7), ISSN 1424-8220. DOI: 10.3390/s17071506. Artikelnr.: 1506., text/html https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-230652 Online-Zugriff |
| spellingShingle |
Schuller, Astrid, Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors, The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity., Diclofenac, Pdr5, Hefe, Biosensor, Technische Universität Dresden, Publikationsfonds, Yeast, Publishing Fund |
| title |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| title_auth |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| title_full |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| title_fullStr |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| title_full_unstemmed |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| title_in_hierarchy |
|
| title_short |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| title_sort |
tuning the sensitivity of the pdr5 promoter-based detection of diclofenac in yeast biosensors |
| title_unstemmed |
Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors |
| topic |
Diclofenac, Pdr5, Hefe, Biosensor, Technische Universität Dresden, Publikationsfonds, Yeast, Publishing Fund |
| topic_facet |
Diclofenac, Pdr5, Hefe, Biosensor, Technische Universität Dresden, Publikationsfonds, Yeast, Publishing Fund |
| url |
https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-230652 |
| urn |
urn:nbn:de:bsz:14-qucosa-230652 |
| work_keys_str_mv |
AT schullerastrid tuningthesensitivityofthepdr5promoterbaseddetectionofdiclofenacinyeastbiosensors, AT rodelgerhard tuningthesensitivityofthepdr5promoterbaseddetectionofdiclofenacinyeastbiosensors, AT ostermannkai tuningthesensitivityofthepdr5promoterbaseddetectionofdiclofenacinyeastbiosensors |
