Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors

Gespeichert in:

Personen und Körperschaften:	Schuller, Astrid, Rödel, Gerhard, Ostermann, Kai
Titel:	Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors
Format:	E-Artikel
Sprache:	Englisch
veröffentlicht:	Basel MDPI (Multidisciplinary Digital Publishing Institute) 2017 Online-Ausg.. 2017
Gesamtaufnahme:	, Sensors (2017), 17(7), ISSN 1424-8220. DOI: 10.3390/s17071506. Artikelnr.: 1506.
Schlagwörter:	Diclofenac Pdr5 Hefe Biosensor Technische Universität Dresden Publikationsfonds Yeast Publishing Fund
Quelle:	Qucosa

Details
Zusammenfassung:	The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity.