author_facet Cloke, Jonathan
Evans, Katharine
Crabtree, David
Hughes, Annette
Simpson, Helen
Leon-Velarde, Carlos
Larson, Nathan
Dave, Keron
Holopainen, Jani
Wickstrand, Nina
Kauppinen, Mikko
Cloke, Jonathan
Evans, Katharine
Crabtree, David
Hughes, Annette
Simpson, Helen
Leon-Velarde, Carlos
Larson, Nathan
Dave, Keron
Holopainen, Jani
Wickstrand, Nina
Kauppinen, Mikko
author Cloke, Jonathan
Evans, Katharine
Crabtree, David
Hughes, Annette
Simpson, Helen
Leon-Velarde, Carlos
Larson, Nathan
Dave, Keron
Holopainen, Jani
Wickstrand, Nina
Kauppinen, Mikko
spellingShingle Cloke, Jonathan
Evans, Katharine
Crabtree, David
Hughes, Annette
Simpson, Helen
Leon-Velarde, Carlos
Larson, Nathan
Dave, Keron
Holopainen, Jani
Wickstrand, Nina
Kauppinen, Mikko
Journal of AOAC INTERNATIONAL
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
Pharmacology
Agronomy and Crop Science
Environmental Chemistry
Food Science
Analytical Chemistry
author_sort cloke, jonathan
spelling Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko 1060-3271 1944-7922 Oxford University Press (OUP) Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry http://dx.doi.org/10.5740/jaoacint.15-044 <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p> Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood Journal of AOAC INTERNATIONAL
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title Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_unstemmed Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_full Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_fullStr Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_full_unstemmed Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_short Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_sort method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood
topic Pharmacology
Agronomy and Crop Science
Environmental Chemistry
Food Science
Analytical Chemistry
url http://dx.doi.org/10.5740/jaoacint.15-044
publishDate 2015
physical 1315-1324
description <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p>
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author Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko
author_facet Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko, Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko
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description <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p>
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spelling Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko 1060-3271 1944-7922 Oxford University Press (OUP) Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry http://dx.doi.org/10.5740/jaoacint.15-044 <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p> Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood Journal of AOAC INTERNATIONAL
spellingShingle Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko, Journal of AOAC INTERNATIONAL, Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood, Pharmacology, Agronomy and Crop Science, Environmental Chemistry, Food Science, Analytical Chemistry
title Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_full Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_fullStr Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_full_unstemmed Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_short Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
title_sort method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood
title_unstemmed Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
topic Pharmacology, Agronomy and Crop Science, Environmental Chemistry, Food Science, Analytical Chemistry
url http://dx.doi.org/10.5740/jaoacint.15-044