Eintrag weiter verarbeiten
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood
Gespeichert in:
Zeitschriftentitel: | Journal of AOAC INTERNATIONAL |
---|---|
Personen und Körperschaften: | , , , , , , , , , , |
In: | Journal of AOAC INTERNATIONAL, 98, 2015, 5, S. 1315-1324 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Oxford University Press (OUP)
|
Schlagwörter: |
author_facet |
Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko |
---|---|
author |
Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko |
spellingShingle |
Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko Journal of AOAC INTERNATIONAL Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
author_sort |
cloke, jonathan |
spelling |
Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko 1060-3271 1944-7922 Oxford University Press (OUP) Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry http://dx.doi.org/10.5740/jaoacint.15-044 <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p> Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood Journal of AOAC INTERNATIONAL |
doi_str_mv |
10.5740/jaoacint.15-044 |
facet_avail |
Online |
finc_class_facet |
Chemie und Pharmazie Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft Technik |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuNTc0MC9qYW9hY2ludC4xNS0wNDQ |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuNTc0MC9qYW9hY2ludC4xNS0wNDQ |
institution |
DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-Ch1 DE-L229 |
imprint |
Oxford University Press (OUP), 2015 |
imprint_str_mv |
Oxford University Press (OUP), 2015 |
issn |
1060-3271 1944-7922 |
issn_str_mv |
1060-3271 1944-7922 |
language |
English |
mega_collection |
Oxford University Press (OUP) (CrossRef) |
match_str |
cloke2015methodmodificationofthethermoscientificsuretectlisteriamonocytogenesassayforrawmeatdairyproduceandseafood |
publishDateSort |
2015 |
publisher |
Oxford University Press (OUP) |
recordtype |
ai |
record_format |
ai |
series |
Journal of AOAC INTERNATIONAL |
source_id |
49 |
title |
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_unstemmed |
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_full |
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_fullStr |
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_full_unstemmed |
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_short |
Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_sort |
method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood |
topic |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
url |
http://dx.doi.org/10.5740/jaoacint.15-044 |
publishDate |
2015 |
physical |
1315-1324 |
description |
<jats:title>Abstract</jats:title>
<jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p> |
container_issue |
5 |
container_start_page |
1315 |
container_title |
Journal of AOAC INTERNATIONAL |
container_volume |
98 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792321400355160075 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T11:01:25.221Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Method+Modification+of+the+Thermo+Scientific+SureTect+Listeria+monocytogenes+Assay+for+Raw+Meat%2C+Dairy%2C+Produce%2C+and+Seafood&rft.date=2015-09-01&genre=article&issn=1944-7922&volume=98&issue=5&spage=1315&epage=1324&pages=1315-1324&jtitle=Journal+of+AOAC+INTERNATIONAL&atitle=Method+Modification+of+the+Thermo+Scientific+SureTect+Listeria+monocytogenes+Assay+for+Raw+Meat%2C+Dairy%2C+Produce%2C+and+Seafood&aulast=Kauppinen&aufirst=Mikko&rft_id=info%3Adoi%2F10.5740%2Fjaoacint.15-044&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792321400355160075 |
author | Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko |
author_facet | Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko, Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko |
author_sort | cloke, jonathan |
container_issue | 5 |
container_start_page | 1315 |
container_title | Journal of AOAC INTERNATIONAL |
container_volume | 98 |
description | <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p> |
doi_str_mv | 10.5740/jaoacint.15-044 |
facet_avail | Online |
finc_class_facet | Chemie und Pharmazie, Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft, Technik |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuNTc0MC9qYW9hY2ludC4xNS0wNDQ |
imprint | Oxford University Press (OUP), 2015 |
imprint_str_mv | Oxford University Press (OUP), 2015 |
institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-Ch1, DE-L229 |
issn | 1060-3271, 1944-7922 |
issn_str_mv | 1060-3271, 1944-7922 |
language | English |
last_indexed | 2024-03-01T11:01:25.221Z |
match_str | cloke2015methodmodificationofthethermoscientificsuretectlisteriamonocytogenesassayforrawmeatdairyproduceandseafood |
mega_collection | Oxford University Press (OUP) (CrossRef) |
physical | 1315-1324 |
publishDate | 2015 |
publishDateSort | 2015 |
publisher | Oxford University Press (OUP) |
record_format | ai |
recordtype | ai |
series | Journal of AOAC INTERNATIONAL |
source_id | 49 |
spelling | Cloke, Jonathan Evans, Katharine Crabtree, David Hughes, Annette Simpson, Helen Leon-Velarde, Carlos Larson, Nathan Dave, Keron Holopainen, Jani Wickstrand, Nina Kauppinen, Mikko 1060-3271 1944-7922 Oxford University Press (OUP) Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry http://dx.doi.org/10.5740/jaoacint.15-044 <jats:title>Abstract</jats:title> <jats:p>The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested MethodSM (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.</jats:p> Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood Journal of AOAC INTERNATIONAL |
spellingShingle | Cloke, Jonathan, Evans, Katharine, Crabtree, David, Hughes, Annette, Simpson, Helen, Leon-Velarde, Carlos, Larson, Nathan, Dave, Keron, Holopainen, Jani, Wickstrand, Nina, Kauppinen, Mikko, Journal of AOAC INTERNATIONAL, Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood, Pharmacology, Agronomy and Crop Science, Environmental Chemistry, Food Science, Analytical Chemistry |
title | Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_full | Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_fullStr | Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_full_unstemmed | Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_short | Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
title_sort | method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood |
title_unstemmed | Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood |
topic | Pharmacology, Agronomy and Crop Science, Environmental Chemistry, Food Science, Analytical Chemistry |
url | http://dx.doi.org/10.5740/jaoacint.15-044 |