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Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran
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| Zeitschriftentitel: | Open Access Macedonian Journal of Medical Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Open Access Macedonian Journal of Medical Sciences, 6, 2018, 12, S. 2277-2282 |
| Format: | E-Article |
| Sprache: | Unbestimmt |
| veröffentlicht: |
Scientific Foundation SPIROSKI
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| Schlagwörter: |
| author_facet |
Anoushirvani, Ali Arash Aghabozorgi, Reza Ahmadi, Azam Arjomandzadegan, Mohammad Sahraei, Maryam Khalili, Sara Fereydouni, Taha Khademi, Zoha Anoushirvani, Ali Arash Aghabozorgi, Reza Ahmadi, Azam Arjomandzadegan, Mohammad Sahraei, Maryam Khalili, Sara Fereydouni, Taha Khademi, Zoha |
|---|---|
| author |
Anoushirvani, Ali Arash Aghabozorgi, Reza Ahmadi, Azam Arjomandzadegan, Mohammad Sahraei, Maryam Khalili, Sara Fereydouni, Taha Khademi, Zoha |
| spellingShingle |
Anoushirvani, Ali Arash Aghabozorgi, Reza Ahmadi, Azam Arjomandzadegan, Mohammad Sahraei, Maryam Khalili, Sara Fereydouni, Taha Khademi, Zoha Open Access Macedonian Journal of Medical Sciences Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran General Medicine |
| author_sort |
anoushirvani, ali arash |
| spelling |
Anoushirvani, Ali Arash Aghabozorgi, Reza Ahmadi, Azam Arjomandzadegan, Mohammad Sahraei, Maryam Khalili, Sara Fereydouni, Taha Khademi, Zoha 1857-9655 Scientific Foundation SPIROSKI General Medicine http://dx.doi.org/10.3889/oamjms.2018.486 <jats:p>BACKGROUND: The nucleotide changes in different genetic loci increased the incidence risk of breast cancer.
 AIM: The aim of present study was to investigate genotype distribution at codon 72 of the TP53 gene (rs1042522) in breast cancer patients to achieve a potential diagnostic marker related to some demographic feathers.
 METHODS: In our case-control study, blood samples were collected from a total of 34 patients harboured breast cancer. DNA was extracted, and nested-PCR was performed. Products were digested with AccII and subsequently were sequenced. Results were compared with samples characteristics.
 RESULTS: The PCR results indicated the correct implementation of extraction and amplification protocol. The genotypic distribution at codon 72 of TP53 in control group was 20%, 62.4% and 16.6% for Arg (wildtype), Arg/Pro (heterozygous) and Pro (homozygous variant) respectively. Also, this distribution in the patient group was 23.52% homozygous, 50% heterozygous, and 26.47% another homozygous variant (Adjusted odds ratio: 1.12 and 95%CI = 0.57 to 2.2, P = 0.03). The absence of Arg at codon 72 of TP53 is relevant with age higher than 40 years and metastasis to other organs.
 CONCLUSION: Polymorphism at codon 72 of TP53 was associated with high-grades of breast cancer risk and different responses to chemotherapy treatment. It is recommended genotype distribution of codon 72 of TP53 before chemotherapy.</jats:p> Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran Open Access Macedonian Journal of Medical Sciences |
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Scientific Foundation SPIROSKI, 2018 |
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1857-9655 |
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Open Access Macedonian Journal of Medical Sciences |
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49 |
| title |
Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_unstemmed |
Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_full |
Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_fullStr |
Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_full_unstemmed |
Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_short |
Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_sort |
association of rs1042522 snp with clinicopathologic factors of breast cancer patients in the markazi province of iran |
| topic |
General Medicine |
| url |
http://dx.doi.org/10.3889/oamjms.2018.486 |
| publishDate |
2018 |
| physical |
2277-2282 |
| description |
<jats:p>BACKGROUND: The nucleotide changes in different genetic loci increased the incidence risk of breast cancer.
AIM: The aim of present study was to investigate genotype distribution at codon 72 of the TP53 gene (rs1042522) in breast cancer patients to achieve a potential diagnostic marker related to some demographic feathers.
METHODS: In our case-control study, blood samples were collected from a total of 34 patients harboured breast cancer. DNA was extracted, and nested-PCR was performed. Products were digested with AccII and subsequently were sequenced. Results were compared with samples characteristics.
RESULTS: The PCR results indicated the correct implementation of extraction and amplification protocol. The genotypic distribution at codon 72 of TP53 in control group was 20%, 62.4% and 16.6% for Arg (wildtype), Arg/Pro (heterozygous) and Pro (homozygous variant) respectively. Also, this distribution in the patient group was 23.52% homozygous, 50% heterozygous, and 26.47% another homozygous variant (Adjusted odds ratio: 1.12 and 95%CI = 0.57 to 2.2, P = 0.03). The absence of Arg at codon 72 of TP53 is relevant with age higher than 40 years and metastasis to other organs.
CONCLUSION: Polymorphism at codon 72 of TP53 was associated with high-grades of breast cancer risk and different responses to chemotherapy treatment. It is recommended genotype distribution of codon 72 of TP53 before chemotherapy.</jats:p> |
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| author | Anoushirvani, Ali Arash, Aghabozorgi, Reza, Ahmadi, Azam, Arjomandzadegan, Mohammad, Sahraei, Maryam, Khalili, Sara, Fereydouni, Taha, Khademi, Zoha |
| author_facet | Anoushirvani, Ali Arash, Aghabozorgi, Reza, Ahmadi, Azam, Arjomandzadegan, Mohammad, Sahraei, Maryam, Khalili, Sara, Fereydouni, Taha, Khademi, Zoha, Anoushirvani, Ali Arash, Aghabozorgi, Reza, Ahmadi, Azam, Arjomandzadegan, Mohammad, Sahraei, Maryam, Khalili, Sara, Fereydouni, Taha, Khademi, Zoha |
| author_sort | anoushirvani, ali arash |
| container_issue | 12 |
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| container_title | Open Access Macedonian Journal of Medical Sciences |
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| description | <jats:p>BACKGROUND: The nucleotide changes in different genetic loci increased the incidence risk of breast cancer.
 AIM: The aim of present study was to investigate genotype distribution at codon 72 of the TP53 gene (rs1042522) in breast cancer patients to achieve a potential diagnostic marker related to some demographic feathers.
 METHODS: In our case-control study, blood samples were collected from a total of 34 patients harboured breast cancer. DNA was extracted, and nested-PCR was performed. Products were digested with AccII and subsequently were sequenced. Results were compared with samples characteristics.
 RESULTS: The PCR results indicated the correct implementation of extraction and amplification protocol. The genotypic distribution at codon 72 of TP53 in control group was 20%, 62.4% and 16.6% for Arg (wildtype), Arg/Pro (heterozygous) and Pro (homozygous variant) respectively. Also, this distribution in the patient group was 23.52% homozygous, 50% heterozygous, and 26.47% another homozygous variant (Adjusted odds ratio: 1.12 and 95%CI = 0.57 to 2.2, P = 0.03). The absence of Arg at codon 72 of TP53 is relevant with age higher than 40 years and metastasis to other organs.
 CONCLUSION: Polymorphism at codon 72 of TP53 was associated with high-grades of breast cancer risk and different responses to chemotherapy treatment. It is recommended genotype distribution of codon 72 of TP53 before chemotherapy.</jats:p> |
| doi_str_mv | 10.3889/oamjms.2018.486 |
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| imprint | Scientific Foundation SPIROSKI, 2018 |
| imprint_str_mv | Scientific Foundation SPIROSKI, 2018 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
| issn | 1857-9655 |
| issn_str_mv | 1857-9655 |
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| publishDate | 2018 |
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| series | Open Access Macedonian Journal of Medical Sciences |
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| spelling | Anoushirvani, Ali Arash Aghabozorgi, Reza Ahmadi, Azam Arjomandzadegan, Mohammad Sahraei, Maryam Khalili, Sara Fereydouni, Taha Khademi, Zoha 1857-9655 Scientific Foundation SPIROSKI General Medicine http://dx.doi.org/10.3889/oamjms.2018.486 <jats:p>BACKGROUND: The nucleotide changes in different genetic loci increased the incidence risk of breast cancer.
 AIM: The aim of present study was to investigate genotype distribution at codon 72 of the TP53 gene (rs1042522) in breast cancer patients to achieve a potential diagnostic marker related to some demographic feathers.
 METHODS: In our case-control study, blood samples were collected from a total of 34 patients harboured breast cancer. DNA was extracted, and nested-PCR was performed. Products were digested with AccII and subsequently were sequenced. Results were compared with samples characteristics.
 RESULTS: The PCR results indicated the correct implementation of extraction and amplification protocol. The genotypic distribution at codon 72 of TP53 in control group was 20%, 62.4% and 16.6% for Arg (wildtype), Arg/Pro (heterozygous) and Pro (homozygous variant) respectively. Also, this distribution in the patient group was 23.52% homozygous, 50% heterozygous, and 26.47% another homozygous variant (Adjusted odds ratio: 1.12 and 95%CI = 0.57 to 2.2, P = 0.03). The absence of Arg at codon 72 of TP53 is relevant with age higher than 40 years and metastasis to other organs.
 CONCLUSION: Polymorphism at codon 72 of TP53 was associated with high-grades of breast cancer risk and different responses to chemotherapy treatment. It is recommended genotype distribution of codon 72 of TP53 before chemotherapy.</jats:p> Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran Open Access Macedonian Journal of Medical Sciences |
| spellingShingle | Anoushirvani, Ali Arash, Aghabozorgi, Reza, Ahmadi, Azam, Arjomandzadegan, Mohammad, Sahraei, Maryam, Khalili, Sara, Fereydouni, Taha, Khademi, Zoha, Open Access Macedonian Journal of Medical Sciences, Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran, General Medicine |
| title | Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_full | Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_fullStr | Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_full_unstemmed | Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_short | Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| title_sort | association of rs1042522 snp with clinicopathologic factors of breast cancer patients in the markazi province of iran |
| title_unstemmed | Association of rs1042522 SNP with Clinicopathologic Factors of Breast Cancer Patients in the Markazi Province of Iran |
| topic | General Medicine |
| url | http://dx.doi.org/10.3889/oamjms.2018.486 |