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An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein
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| Zeitschriftentitel: | Sensors |
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| Personen und Körperschaften: | , , , , , , , , |
| In: | Sensors, 20, 2020, 3, S. 617 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
MDPI AG
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| Schlagwörter: |
| author_facet |
Ge, Chuang-Ye Rahman, Md. Mahbubur Zhang, Wei Lopa, Nasrin Siraj Jin, Lei Yoon, Sujin Jang, Hohyoun Xu, Guang-Ri Kim, Whangi Ge, Chuang-Ye Rahman, Md. Mahbubur Zhang, Wei Lopa, Nasrin Siraj Jin, Lei Yoon, Sujin Jang, Hohyoun Xu, Guang-Ri Kim, Whangi |
|---|---|
| author |
Ge, Chuang-Ye Rahman, Md. Mahbubur Zhang, Wei Lopa, Nasrin Siraj Jin, Lei Yoon, Sujin Jang, Hohyoun Xu, Guang-Ri Kim, Whangi |
| spellingShingle |
Ge, Chuang-Ye Rahman, Md. Mahbubur Zhang, Wei Lopa, Nasrin Siraj Jin, Lei Yoon, Sujin Jang, Hohyoun Xu, Guang-Ri Kim, Whangi Sensors An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein Electrical and Electronic Engineering Biochemistry Instrumentation Atomic and Molecular Physics, and Optics Analytical Chemistry |
| author_sort |
ge, chuang-ye |
| spelling |
Ge, Chuang-Ye Rahman, Md. Mahbubur Zhang, Wei Lopa, Nasrin Siraj Jin, Lei Yoon, Sujin Jang, Hohyoun Xu, Guang-Ri Kim, Whangi 1424-8220 MDPI AG Electrical and Electronic Engineering Biochemistry Instrumentation Atomic and Molecular Physics, and Optics Analytical Chemistry http://dx.doi.org/10.3390/s20030617 <jats:p>This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of α-synuclein (α-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (–NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-α-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with α-Syn-antigen. The variation of the concentrations of the attached α-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (Rct) for the redox response of [Fe(CN)6]3−/4−, which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of α-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect α-Syn for the early diagnose of Parkinson’s disease, which can be extended for the determination of other biologically important biomarkers.</jats:p> An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein Sensors |
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| title |
An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_unstemmed |
An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_full |
An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_fullStr |
An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_full_unstemmed |
An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_short |
An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_sort |
an electrochemical immunosensor based on a self-assembled monolayer modified electrode for label-free detection of α-synuclein |
| topic |
Electrical and Electronic Engineering Biochemistry Instrumentation Atomic and Molecular Physics, and Optics Analytical Chemistry |
| url |
http://dx.doi.org/10.3390/s20030617 |
| publishDate |
2020 |
| physical |
617 |
| description |
<jats:p>This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of α-synuclein (α-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (–NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-α-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with α-Syn-antigen. The variation of the concentrations of the attached α-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (Rct) for the redox response of [Fe(CN)6]3−/4−, which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of α-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect α-Syn for the early diagnose of Parkinson’s disease, which can be extended for the determination of other biologically important biomarkers.</jats:p> |
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| author | Ge, Chuang-Ye, Rahman, Md. Mahbubur, Zhang, Wei, Lopa, Nasrin Siraj, Jin, Lei, Yoon, Sujin, Jang, Hohyoun, Xu, Guang-Ri, Kim, Whangi |
| author_facet | Ge, Chuang-Ye, Rahman, Md. Mahbubur, Zhang, Wei, Lopa, Nasrin Siraj, Jin, Lei, Yoon, Sujin, Jang, Hohyoun, Xu, Guang-Ri, Kim, Whangi, Ge, Chuang-Ye, Rahman, Md. Mahbubur, Zhang, Wei, Lopa, Nasrin Siraj, Jin, Lei, Yoon, Sujin, Jang, Hohyoun, Xu, Guang-Ri, Kim, Whangi |
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| description | <jats:p>This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of α-synuclein (α-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (–NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-α-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with α-Syn-antigen. The variation of the concentrations of the attached α-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (Rct) for the redox response of [Fe(CN)6]3−/4−, which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of α-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect α-Syn for the early diagnose of Parkinson’s disease, which can be extended for the determination of other biologically important biomarkers.</jats:p> |
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| spelling | Ge, Chuang-Ye Rahman, Md. Mahbubur Zhang, Wei Lopa, Nasrin Siraj Jin, Lei Yoon, Sujin Jang, Hohyoun Xu, Guang-Ri Kim, Whangi 1424-8220 MDPI AG Electrical and Electronic Engineering Biochemistry Instrumentation Atomic and Molecular Physics, and Optics Analytical Chemistry http://dx.doi.org/10.3390/s20030617 <jats:p>This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of α-synuclein (α-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (–NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-α-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with α-Syn-antigen. The variation of the concentrations of the attached α-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (Rct) for the redox response of [Fe(CN)6]3−/4−, which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of α-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect α-Syn for the early diagnose of Parkinson’s disease, which can be extended for the determination of other biologically important biomarkers.</jats:p> An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein Sensors |
| spellingShingle | Ge, Chuang-Ye, Rahman, Md. Mahbubur, Zhang, Wei, Lopa, Nasrin Siraj, Jin, Lei, Yoon, Sujin, Jang, Hohyoun, Xu, Guang-Ri, Kim, Whangi, Sensors, An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein, Electrical and Electronic Engineering, Biochemistry, Instrumentation, Atomic and Molecular Physics, and Optics, Analytical Chemistry |
| title | An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_full | An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_fullStr | An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_full_unstemmed | An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_short | An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| title_sort | an electrochemical immunosensor based on a self-assembled monolayer modified electrode for label-free detection of α-synuclein |
| title_unstemmed | An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein |
| topic | Electrical and Electronic Engineering, Biochemistry, Instrumentation, Atomic and Molecular Physics, and Optics, Analytical Chemistry |
| url | http://dx.doi.org/10.3390/s20030617 |