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An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids
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| Zeitschriftentitel: | Molecules |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecules, 24, 2019, 19, S. 3516 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
MDPI AG
|
| Schlagwörter: |
| author_facet |
Li, Jing He, Xianqing Deng, Yuanyuan Yang, Chenxi Li, Jing He, Xianqing Deng, Yuanyuan Yang, Chenxi |
|---|---|
| author |
Li, Jing He, Xianqing Deng, Yuanyuan Yang, Chenxi |
| spellingShingle |
Li, Jing He, Xianqing Deng, Yuanyuan Yang, Chenxi Molecules An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science |
| author_sort |
li, jing |
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Li, Jing He, Xianqing Deng, Yuanyuan Yang, Chenxi 1420-3049 MDPI AG Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science http://dx.doi.org/10.3390/molecules24193516 <jats:p>Extracellular vesicles (EVs) are lipid bilayer enclosed particles which present in almost all types of biofluids and contain specific proteins, lipids, and RNA. Increasing evidence has demonstrated the tremendous clinical potential of EVs as diagnostic and therapeutic tools, especially in biofluids, since they can be detected without invasive surgery. With the advanced mass spectrometry (MS), it is possible to decipher the protein content of EVs under different physiological and pathological conditions. Therefore, MS-based EV proteomic studies have grown rapidly in the past decade for biomarker discovery. This review focuses on the studies that isolate EVs from different biofluids and contain MS-based proteomic analysis. Literature published in the past decade (2009.1–2019.7) were selected and summarized with emphasis on isolation methods of EVs and MS analysis strategies, with the aim to give an overview of MS-based EV proteomic studies and provide a reference for future research.</jats:p> An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids Molecules |
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| title |
An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_unstemmed |
An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_full |
An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_fullStr |
An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_full_unstemmed |
An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_short |
An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_sort |
an update on isolation methods for proteomic studies of extracellular vesicles in biofluids |
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Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science |
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<jats:p>Extracellular vesicles (EVs) are lipid bilayer enclosed particles which present in almost all types of biofluids and contain specific proteins, lipids, and RNA. Increasing evidence has demonstrated the tremendous clinical potential of EVs as diagnostic and therapeutic tools, especially in biofluids, since they can be detected without invasive surgery. With the advanced mass spectrometry (MS), it is possible to decipher the protein content of EVs under different physiological and pathological conditions. Therefore, MS-based EV proteomic studies have grown rapidly in the past decade for biomarker discovery. This review focuses on the studies that isolate EVs from different biofluids and contain MS-based proteomic analysis. Literature published in the past decade (2009.1–2019.7) were selected and summarized with emphasis on isolation methods of EVs and MS analysis strategies, with the aim to give an overview of MS-based EV proteomic studies and provide a reference for future research.</jats:p> |
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| description | <jats:p>Extracellular vesicles (EVs) are lipid bilayer enclosed particles which present in almost all types of biofluids and contain specific proteins, lipids, and RNA. Increasing evidence has demonstrated the tremendous clinical potential of EVs as diagnostic and therapeutic tools, especially in biofluids, since they can be detected without invasive surgery. With the advanced mass spectrometry (MS), it is possible to decipher the protein content of EVs under different physiological and pathological conditions. Therefore, MS-based EV proteomic studies have grown rapidly in the past decade for biomarker discovery. This review focuses on the studies that isolate EVs from different biofluids and contain MS-based proteomic analysis. Literature published in the past decade (2009.1–2019.7) were selected and summarized with emphasis on isolation methods of EVs and MS analysis strategies, with the aim to give an overview of MS-based EV proteomic studies and provide a reference for future research.</jats:p> |
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| spelling | Li, Jing He, Xianqing Deng, Yuanyuan Yang, Chenxi 1420-3049 MDPI AG Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science http://dx.doi.org/10.3390/molecules24193516 <jats:p>Extracellular vesicles (EVs) are lipid bilayer enclosed particles which present in almost all types of biofluids and contain specific proteins, lipids, and RNA. Increasing evidence has demonstrated the tremendous clinical potential of EVs as diagnostic and therapeutic tools, especially in biofluids, since they can be detected without invasive surgery. With the advanced mass spectrometry (MS), it is possible to decipher the protein content of EVs under different physiological and pathological conditions. Therefore, MS-based EV proteomic studies have grown rapidly in the past decade for biomarker discovery. This review focuses on the studies that isolate EVs from different biofluids and contain MS-based proteomic analysis. Literature published in the past decade (2009.1–2019.7) were selected and summarized with emphasis on isolation methods of EVs and MS analysis strategies, with the aim to give an overview of MS-based EV proteomic studies and provide a reference for future research.</jats:p> An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids Molecules |
| spellingShingle | Li, Jing, He, Xianqing, Deng, Yuanyuan, Yang, Chenxi, Molecules, An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids, Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science |
| title | An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_full | An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_fullStr | An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_full_unstemmed | An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_short | An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| title_sort | an update on isolation methods for proteomic studies of extracellular vesicles in biofluids |
| title_unstemmed | An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids |
| topic | Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science |
| url | http://dx.doi.org/10.3390/molecules24193516 |