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| author_facet |
Li, Xiaoqing Lu, Ang Deng, Wenjie Su, Li Wang, Jing Ding, Mingyue Li, Xiaoqing Lu, Ang Deng, Wenjie Su, Li Wang, Jing Ding, Mingyue |
|---|---|
| author |
Li, Xiaoqing Lu, Ang Deng, Wenjie Su, Li Wang, Jing Ding, Mingyue |
| spellingShingle |
Li, Xiaoqing Lu, Ang Deng, Wenjie Su, Li Wang, Jing Ding, Mingyue Cells Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) General Medicine |
| author_sort |
li, xiaoqing |
| spelling |
Li, Xiaoqing Lu, Ang Deng, Wenjie Su, Li Wang, Jing Ding, Mingyue 2073-4409 MDPI AG General Medicine http://dx.doi.org/10.3390/cells8040314 <jats:p>We report an imaging approach applying the atomic force acoustic microscopy (AFAM), which has unique potential for nondestructive imaging of cell internal structures. To obtain high spatial resolution images, we optimized the significant imaging parameters, including scanning speeds, feedback configurations and acoustic frequencies of an AFAM system, to increase the amplitude of the acoustic signal and to stabilize the morphological signals. We also combined the acoustic amplitude and phase signals, and generated pseudo-color figures for better illustration of subcellular features such as pseudopodia, membranes and nucleus-like. The subcellular structural image atlas can describe nanoscale details of multiple samples and provide clearer images of the subcellular features compared to other conventional techniques. This study builds a strong basis of transmission AFAM for cell imaging, which can help researchers to clarify the cell structures in diverse biological fields and push the understanding of biology evolution to a new stage.</jats:p> Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) Cells |
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10.3390/cells8040314 |
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DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 |
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MDPI AG, 2019 |
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MDPI AG, 2019 |
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2073-4409 |
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2073-4409 |
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MDPI AG |
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Cells |
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49 |
| title |
Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_unstemmed |
Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_full |
Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_fullStr |
Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_full_unstemmed |
Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_short |
Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_sort |
noninvasive subcellular imaging using atomic force acoustic microscopy (afam) |
| topic |
General Medicine |
| url |
http://dx.doi.org/10.3390/cells8040314 |
| publishDate |
2019 |
| physical |
314 |
| description |
<jats:p>We report an imaging approach applying the atomic force acoustic microscopy (AFAM), which has unique potential for nondestructive imaging of cell internal structures. To obtain high spatial resolution images, we optimized the significant imaging parameters, including scanning speeds, feedback configurations and acoustic frequencies of an AFAM system, to increase the amplitude of the acoustic signal and to stabilize the morphological signals. We also combined the acoustic amplitude and phase signals, and generated pseudo-color figures for better illustration of subcellular features such as pseudopodia, membranes and nucleus-like. The subcellular structural image atlas can describe nanoscale details of multiple samples and provide clearer images of the subcellular features compared to other conventional techniques. This study builds a strong basis of transmission AFAM for cell imaging, which can help researchers to clarify the cell structures in diverse biological fields and push the understanding of biology evolution to a new stage.</jats:p> |
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| author | Li, Xiaoqing, Lu, Ang, Deng, Wenjie, Su, Li, Wang, Jing, Ding, Mingyue |
| author_facet | Li, Xiaoqing, Lu, Ang, Deng, Wenjie, Su, Li, Wang, Jing, Ding, Mingyue, Li, Xiaoqing, Lu, Ang, Deng, Wenjie, Su, Li, Wang, Jing, Ding, Mingyue |
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| description | <jats:p>We report an imaging approach applying the atomic force acoustic microscopy (AFAM), which has unique potential for nondestructive imaging of cell internal structures. To obtain high spatial resolution images, we optimized the significant imaging parameters, including scanning speeds, feedback configurations and acoustic frequencies of an AFAM system, to increase the amplitude of the acoustic signal and to stabilize the morphological signals. We also combined the acoustic amplitude and phase signals, and generated pseudo-color figures for better illustration of subcellular features such as pseudopodia, membranes and nucleus-like. The subcellular structural image atlas can describe nanoscale details of multiple samples and provide clearer images of the subcellular features compared to other conventional techniques. This study builds a strong basis of transmission AFAM for cell imaging, which can help researchers to clarify the cell structures in diverse biological fields and push the understanding of biology evolution to a new stage.</jats:p> |
| doi_str_mv | 10.3390/cells8040314 |
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| imprint | MDPI AG, 2019 |
| imprint_str_mv | MDPI AG, 2019 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
| issn | 2073-4409 |
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| language | English |
| last_indexed | 2024-03-01T16:35:56.102Z |
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| mega_collection | MDPI AG (CrossRef) |
| physical | 314 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI AG |
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| recordtype | ai |
| series | Cells |
| source_id | 49 |
| spelling | Li, Xiaoqing Lu, Ang Deng, Wenjie Su, Li Wang, Jing Ding, Mingyue 2073-4409 MDPI AG General Medicine http://dx.doi.org/10.3390/cells8040314 <jats:p>We report an imaging approach applying the atomic force acoustic microscopy (AFAM), which has unique potential for nondestructive imaging of cell internal structures. To obtain high spatial resolution images, we optimized the significant imaging parameters, including scanning speeds, feedback configurations and acoustic frequencies of an AFAM system, to increase the amplitude of the acoustic signal and to stabilize the morphological signals. We also combined the acoustic amplitude and phase signals, and generated pseudo-color figures for better illustration of subcellular features such as pseudopodia, membranes and nucleus-like. The subcellular structural image atlas can describe nanoscale details of multiple samples and provide clearer images of the subcellular features compared to other conventional techniques. This study builds a strong basis of transmission AFAM for cell imaging, which can help researchers to clarify the cell structures in diverse biological fields and push the understanding of biology evolution to a new stage.</jats:p> Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) Cells |
| spellingShingle | Li, Xiaoqing, Lu, Ang, Deng, Wenjie, Su, Li, Wang, Jing, Ding, Mingyue, Cells, Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM), General Medicine |
| title | Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_full | Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_fullStr | Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_full_unstemmed | Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_short | Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| title_sort | noninvasive subcellular imaging using atomic force acoustic microscopy (afam) |
| title_unstemmed | Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) |
| topic | General Medicine |
| url | http://dx.doi.org/10.3390/cells8040314 |