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Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study
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| Zeitschriftentitel: | The EuroBiotech Journal |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | The EuroBiotech Journal, 1, 2017, 4, S. 310-318 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Walter de Gruyter GmbH
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| Schlagwörter: |
| author_facet |
Marova, Ivana Szotkowski, Martin Vanek, Martin Rapta, Marek Byrtusova, Dana Mikheichyk, Nadzeya Haronikova, Andrea Certik, Milan Shapaval, Volha Marova, Ivana Szotkowski, Martin Vanek, Martin Rapta, Marek Byrtusova, Dana Mikheichyk, Nadzeya Haronikova, Andrea Certik, Milan Shapaval, Volha |
|---|---|
| author |
Marova, Ivana Szotkowski, Martin Vanek, Martin Rapta, Marek Byrtusova, Dana Mikheichyk, Nadzeya Haronikova, Andrea Certik, Milan Shapaval, Volha |
| spellingShingle |
Marova, Ivana Szotkowski, Martin Vanek, Martin Rapta, Marek Byrtusova, Dana Mikheichyk, Nadzeya Haronikova, Andrea Certik, Milan Shapaval, Volha The EuroBiotech Journal Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology |
| author_sort |
marova, ivana |
| spelling |
Marova, Ivana Szotkowski, Martin Vanek, Martin Rapta, Marek Byrtusova, Dana Mikheichyk, Nadzeya Haronikova, Andrea Certik, Milan Shapaval, Volha 2564-615X Walter de Gruyter GmbH Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology http://dx.doi.org/10.24190/issn2564-615x/2017/04.08 <jats:title>Abstract</jats:title> <jats:p> Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetrically. All tested strains utilized fat hydrolysate and produced red coloured biomass. Cultivation in media containing non-hydrolysed fat led to strain specific induction of extracellular lipase. Amount of lipid metabolites produced by individual strains was depended on glycerol content in medium. The highest increase of lipase production was observed in Cystofilobasidium macerans and Sporobolomyces shibatanus. Valorisation of animal fat can lead to production of unsaturated fatty acids, single cell oils, carotenoid pigments, sterols and enriched red yeast biomass.</jats:p> Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study The EuroBiotech Journal |
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Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_unstemmed |
Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_full |
Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_fullStr |
Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_full_unstemmed |
Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_short |
Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_sort |
utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
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Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology |
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http://dx.doi.org/10.24190/issn2564-615x/2017/04.08 |
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2017 |
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310-318 |
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<jats:title>Abstract</jats:title>
<jats:p> Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetrically. All tested strains utilized fat hydrolysate and produced red coloured biomass. Cultivation in media containing non-hydrolysed fat led to strain specific induction of extracellular lipase. Amount of lipid metabolites produced by individual strains was depended on glycerol content in medium. The highest increase of lipase production was observed in Cystofilobasidium macerans and Sporobolomyces shibatanus. Valorisation of animal fat can lead to production of unsaturated fatty acids, single cell oils, carotenoid pigments, sterols and enriched red yeast biomass.</jats:p> |
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| author | Marova, Ivana, Szotkowski, Martin, Vanek, Martin, Rapta, Marek, Byrtusova, Dana, Mikheichyk, Nadzeya, Haronikova, Andrea, Certik, Milan, Shapaval, Volha |
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| description | <jats:title>Abstract</jats:title> <jats:p> Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetrically. All tested strains utilized fat hydrolysate and produced red coloured biomass. Cultivation in media containing non-hydrolysed fat led to strain specific induction of extracellular lipase. Amount of lipid metabolites produced by individual strains was depended on glycerol content in medium. The highest increase of lipase production was observed in Cystofilobasidium macerans and Sporobolomyces shibatanus. Valorisation of animal fat can lead to production of unsaturated fatty acids, single cell oils, carotenoid pigments, sterols and enriched red yeast biomass.</jats:p> |
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| imprint_str_mv | Walter de Gruyter GmbH, 2017 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Marova, Ivana Szotkowski, Martin Vanek, Martin Rapta, Marek Byrtusova, Dana Mikheichyk, Nadzeya Haronikova, Andrea Certik, Milan Shapaval, Volha 2564-615X Walter de Gruyter GmbH Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology http://dx.doi.org/10.24190/issn2564-615x/2017/04.08 <jats:title>Abstract</jats:title> <jats:p> Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetrically. All tested strains utilized fat hydrolysate and produced red coloured biomass. Cultivation in media containing non-hydrolysed fat led to strain specific induction of extracellular lipase. Amount of lipid metabolites produced by individual strains was depended on glycerol content in medium. The highest increase of lipase production was observed in Cystofilobasidium macerans and Sporobolomyces shibatanus. Valorisation of animal fat can lead to production of unsaturated fatty acids, single cell oils, carotenoid pigments, sterols and enriched red yeast biomass.</jats:p> Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study The EuroBiotech Journal |
| spellingShingle | Marova, Ivana, Szotkowski, Martin, Vanek, Martin, Rapta, Marek, Byrtusova, Dana, Mikheichyk, Nadzeya, Haronikova, Andrea, Certik, Milan, Shapaval, Volha, The EuroBiotech Journal, Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study, Genetics, Molecular Biology, Biomedical Engineering, Molecular Medicine, Food Science, Biotechnology |
| title | Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_full | Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_fullStr | Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_full_unstemmed | Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_short | Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_sort | utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| title_unstemmed | Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study |
| topic | Genetics, Molecular Biology, Biomedical Engineering, Molecular Medicine, Food Science, Biotechnology |
| url | http://dx.doi.org/10.24190/issn2564-615x/2017/04.08 |