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Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Cont...
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| Zeitschriftentitel: | The Journal of Neuroscience |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | The Journal of Neuroscience, 24, 2004, 48, S. 10868-10877 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Society for Neuroscience
|
| Schlagwörter: |
| author_facet |
Li, Qian Holmes, Andrew Ma, Li Van de Kar, Louis D. Garcia, Francisca Murphy, Dennis L. Li, Qian Holmes, Andrew Ma, Li Van de Kar, Louis D. Garcia, Francisca Murphy, Dennis L. |
|---|---|
| author |
Li, Qian Holmes, Andrew Ma, Li Van de Kar, Louis D. Garcia, Francisca Murphy, Dennis L. |
| spellingShingle |
Li, Qian Holmes, Andrew Ma, Li Van de Kar, Louis D. Garcia, Francisca Murphy, Dennis L. The Journal of Neuroscience Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences General Neuroscience |
| author_sort |
li, qian |
| spelling |
Li, Qian Holmes, Andrew Ma, Li Van de Kar, Louis D. Garcia, Francisca Murphy, Dennis L. 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.3223-04.2004 <jats:p>Our previous studies found that serotonin transporter (SERT) knock-out mice showed increased sensitivity to minor stress and increased anxiety-like behavior but reduced locomotor activity. These mice also showed decreased density of 5-hydroxytryptamine (5-HT<jats:sub>1A</jats:sub>) receptors in the hypothalamus, amygdala, and dorsal raphe. To evaluate the contribution of hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors to these phenotypes of SERT knock-out mice, two studies were conducted. Recombinant adenoviruses containing 5-HT<jats:sub>1A</jats:sub>sense and antisense sequences (Ad-1AP-sense and Ad-1AP-antisense) were used to manipulate 5-HT<jats:sub>1A</jats:sub>receptors in the hypothalamus. The expression of the 5-HT<jats:sub>1A</jats:sub>genes is controlled by the 5-HT<jats:sub>1A</jats:sub>promoter, so that they are only expressed in 5-HT<jats:sub>1A</jats:sub>receptor-containing cells. (1) Injection of Ad-1AP-sense into the hypothalamus of SERT knock-out mice restored 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus; this effect was accompanied by elimination of the exaggerated adrenocorticotropin responses to a saline injection (minor stress) and reduced locomotor activity but not by a change in increased exploratory anxiety-like behavior. (2) To further confirm the observation in SERT<jats:sup>-/-</jats:sup>mice, Ad-1AP-antisense was injected into the hypothalamus of normal mice. The density and the function of 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus were significantly reduced in Ad-1AP-antisense-treated mice. Compared with the control group (injected with Ad-track), Ad-1A-antisense-treated mice showed a significant reduction in locomotor activity, but again no changes in exploratory anxiety-like behaviors, tested by elevated plus-maze and open-field tests. Thus, the present results demonstrate that medial hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors regulate stress responses and locomotor activity but may not regulate exploratory anxiety-like behaviors.</jats:p> Medial Hypothalamic 5-Hydroxytryptamine (5-HT)<sub>1A</sub>Receptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT<sub>1A</sub>Sequences The Journal of Neuroscience |
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49 |
| title |
Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_unstemmed |
Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_full |
Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_fullStr |
Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_full_unstemmed |
Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_short |
Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_sort |
medial hypothalamic 5-hydroxytryptamine (5-ht)<sub>1a</sub>receptors regulate neuroendocrine responses to stress and exploratory locomotor activity: application of recombinant adenovirus containing 5-ht<sub>1a</sub>sequences |
| topic |
General Neuroscience |
| url |
http://dx.doi.org/10.1523/jneurosci.3223-04.2004 |
| publishDate |
2004 |
| physical |
10868-10877 |
| description |
<jats:p>Our previous studies found that serotonin transporter (SERT) knock-out mice showed increased sensitivity to minor stress and increased anxiety-like behavior but reduced locomotor activity. These mice also showed decreased density of 5-hydroxytryptamine (5-HT<jats:sub>1A</jats:sub>) receptors in the hypothalamus, amygdala, and dorsal raphe. To evaluate the contribution of hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors to these phenotypes of SERT knock-out mice, two studies were conducted. Recombinant adenoviruses containing 5-HT<jats:sub>1A</jats:sub>sense and antisense sequences (Ad-1AP-sense and Ad-1AP-antisense) were used to manipulate 5-HT<jats:sub>1A</jats:sub>receptors in the hypothalamus. The expression of the 5-HT<jats:sub>1A</jats:sub>genes is controlled by the 5-HT<jats:sub>1A</jats:sub>promoter, so that they are only expressed in 5-HT<jats:sub>1A</jats:sub>receptor-containing cells. (1) Injection of Ad-1AP-sense into the hypothalamus of SERT knock-out mice restored 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus; this effect was accompanied by elimination of the exaggerated adrenocorticotropin responses to a saline injection (minor stress) and reduced locomotor activity but not by a change in increased exploratory anxiety-like behavior. (2) To further confirm the observation in SERT<jats:sup>-/-</jats:sup>mice, Ad-1AP-antisense was injected into the hypothalamus of normal mice. The density and the function of 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus were significantly reduced in Ad-1AP-antisense-treated mice. Compared with the control group (injected with Ad-track), Ad-1A-antisense-treated mice showed a significant reduction in locomotor activity, but again no changes in exploratory anxiety-like behaviors, tested by elevated plus-maze and open-field tests. Thus, the present results demonstrate that medial hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors regulate stress responses and locomotor activity but may not regulate exploratory anxiety-like behaviors.</jats:p> |
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| author | Li, Qian, Holmes, Andrew, Ma, Li, Van de Kar, Louis D., Garcia, Francisca, Murphy, Dennis L. |
| author_facet | Li, Qian, Holmes, Andrew, Ma, Li, Van de Kar, Louis D., Garcia, Francisca, Murphy, Dennis L., Li, Qian, Holmes, Andrew, Ma, Li, Van de Kar, Louis D., Garcia, Francisca, Murphy, Dennis L. |
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| description | <jats:p>Our previous studies found that serotonin transporter (SERT) knock-out mice showed increased sensitivity to minor stress and increased anxiety-like behavior but reduced locomotor activity. These mice also showed decreased density of 5-hydroxytryptamine (5-HT<jats:sub>1A</jats:sub>) receptors in the hypothalamus, amygdala, and dorsal raphe. To evaluate the contribution of hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors to these phenotypes of SERT knock-out mice, two studies were conducted. Recombinant adenoviruses containing 5-HT<jats:sub>1A</jats:sub>sense and antisense sequences (Ad-1AP-sense and Ad-1AP-antisense) were used to manipulate 5-HT<jats:sub>1A</jats:sub>receptors in the hypothalamus. The expression of the 5-HT<jats:sub>1A</jats:sub>genes is controlled by the 5-HT<jats:sub>1A</jats:sub>promoter, so that they are only expressed in 5-HT<jats:sub>1A</jats:sub>receptor-containing cells. (1) Injection of Ad-1AP-sense into the hypothalamus of SERT knock-out mice restored 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus; this effect was accompanied by elimination of the exaggerated adrenocorticotropin responses to a saline injection (minor stress) and reduced locomotor activity but not by a change in increased exploratory anxiety-like behavior. (2) To further confirm the observation in SERT<jats:sup>-/-</jats:sup>mice, Ad-1AP-antisense was injected into the hypothalamus of normal mice. The density and the function of 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus were significantly reduced in Ad-1AP-antisense-treated mice. Compared with the control group (injected with Ad-track), Ad-1A-antisense-treated mice showed a significant reduction in locomotor activity, but again no changes in exploratory anxiety-like behaviors, tested by elevated plus-maze and open-field tests. Thus, the present results demonstrate that medial hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors regulate stress responses and locomotor activity but may not regulate exploratory anxiety-like behaviors.</jats:p> |
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| spelling | Li, Qian Holmes, Andrew Ma, Li Van de Kar, Louis D. Garcia, Francisca Murphy, Dennis L. 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.3223-04.2004 <jats:p>Our previous studies found that serotonin transporter (SERT) knock-out mice showed increased sensitivity to minor stress and increased anxiety-like behavior but reduced locomotor activity. These mice also showed decreased density of 5-hydroxytryptamine (5-HT<jats:sub>1A</jats:sub>) receptors in the hypothalamus, amygdala, and dorsal raphe. To evaluate the contribution of hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors to these phenotypes of SERT knock-out mice, two studies were conducted. Recombinant adenoviruses containing 5-HT<jats:sub>1A</jats:sub>sense and antisense sequences (Ad-1AP-sense and Ad-1AP-antisense) were used to manipulate 5-HT<jats:sub>1A</jats:sub>receptors in the hypothalamus. The expression of the 5-HT<jats:sub>1A</jats:sub>genes is controlled by the 5-HT<jats:sub>1A</jats:sub>promoter, so that they are only expressed in 5-HT<jats:sub>1A</jats:sub>receptor-containing cells. (1) Injection of Ad-1AP-sense into the hypothalamus of SERT knock-out mice restored 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus; this effect was accompanied by elimination of the exaggerated adrenocorticotropin responses to a saline injection (minor stress) and reduced locomotor activity but not by a change in increased exploratory anxiety-like behavior. (2) To further confirm the observation in SERT<jats:sup>-/-</jats:sup>mice, Ad-1AP-antisense was injected into the hypothalamus of normal mice. The density and the function of 5-HT<jats:sub>1A</jats:sub>receptors in the medial hypothalamus were significantly reduced in Ad-1AP-antisense-treated mice. Compared with the control group (injected with Ad-track), Ad-1A-antisense-treated mice showed a significant reduction in locomotor activity, but again no changes in exploratory anxiety-like behaviors, tested by elevated plus-maze and open-field tests. Thus, the present results demonstrate that medial hypothalamic 5-HT<jats:sub>1A</jats:sub>receptors regulate stress responses and locomotor activity but may not regulate exploratory anxiety-like behaviors.</jats:p> Medial Hypothalamic 5-Hydroxytryptamine (5-HT)<sub>1A</sub>Receptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT<sub>1A</sub>Sequences The Journal of Neuroscience |
| spellingShingle | Li, Qian, Holmes, Andrew, Ma, Li, Van de Kar, Louis D., Garcia, Francisca, Murphy, Dennis L., The Journal of Neuroscience, Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences, General Neuroscience |
| title | Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_full | Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_fullStr | Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_full_unstemmed | Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_short | Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| title_sort | medial hypothalamic 5-hydroxytryptamine (5-ht)<sub>1a</sub>receptors regulate neuroendocrine responses to stress and exploratory locomotor activity: application of recombinant adenovirus containing 5-ht<sub>1a</sub>sequences |
| title_unstemmed | Medial Hypothalamic 5-Hydroxytryptamine (5-HT)1AReceptors Regulate Neuroendocrine Responses to Stress and Exploratory Locomotor Activity: Application of Recombinant Adenovirus Containing 5-HT1ASequences |
| topic | General Neuroscience |
| url | http://dx.doi.org/10.1523/jneurosci.3223-04.2004 |