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Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network
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| Zeitschriftentitel: | The Journal of Neuroscience |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | The Journal of Neuroscience, 23, 2003, 27, S. 9059-9067 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Society for Neuroscience
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| Schlagwörter: |
| author_facet |
Zhang, Ying Oliva, Ricardo Gisselmann, Günter Hatt, Hanns Guckenheimer, John Harris-Warrick, Ronald M. Zhang, Ying Oliva, Ricardo Gisselmann, Günter Hatt, Hanns Guckenheimer, John Harris-Warrick, Ronald M. |
|---|---|
| author |
Zhang, Ying Oliva, Ricardo Gisselmann, Günter Hatt, Hanns Guckenheimer, John Harris-Warrick, Ronald M. |
| spellingShingle |
Zhang, Ying Oliva, Ricardo Gisselmann, Günter Hatt, Hanns Guckenheimer, John Harris-Warrick, Ronald M. The Journal of Neuroscience Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network General Neuroscience |
| author_sort |
zhang, ying |
| spelling |
Zhang, Ying Oliva, Ricardo Gisselmann, Günter Hatt, Hanns Guckenheimer, John Harris-Warrick, Ronald M. 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.23-27-09059.2003 <jats:p>The hyperpolarization-activated cation current (<jats:italic>I</jats:italic><jats:sub>h</jats:sub>) is widely distributed in excitable cells.<jats:italic>I</jats:italic><jats:sub>h</jats:sub>plays important roles in regulation of cellular excitability, rhythmic activity, and synaptic function. We previously showed that, in pyloric dilator (PD) neurons of the stomatogastric ganglion (STG) of spiny lobsters,<jats:italic>I</jats:italic><jats:sub>h</jats:sub>can be endogenously upregulated to compensate for artificial overexpression of the Shal transient potassium channel; this maintains normal firing properties of the neuron despite large increases in potassium current. To further explore the function of<jats:italic>I</jats:italic><jats:sub>h</jats:sub>in the pyloric network, we injected cRNA of<jats:italic>PAIH</jats:italic>, a lobster gene that encodes<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, into rhythmically active PD neurons. Overexpression of PAIH produced a fourfold increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, although with somewhat different biophysical properties than the endogenous current. Compared with the endogenous<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, the voltage for half-maximal activation of the PAIH-evoked current was depolarized by 10 mV, and its activation kinetics were significantly faster. This increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>did not affect the expression of<jats:italic>I</jats:italic><jats:sub>A</jats:sub>or other outward currents. Instead, it significantly altered the firing properties of the PD neurons. Increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>depolarized the minimum membrane potential of the cell, reduced the oscillation amplitude, decreased the time to the first spike, and increased the duty cycle and number of action potentials per burst. We used both dynamic-clamp experiments, injecting the modeled PAIH currents into PD cells in a functioning STG, and a theoretical model of a two-cell network to demonstrate that the increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>was sufficient to cause the observed changes in the PD activity.</jats:p> Overexpression of a Hyperpolarization-Activated Cation Current (<i>I</i><sub>h</sub>) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network The Journal of Neuroscience |
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2003 |
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Society for Neuroscience |
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The Journal of Neuroscience |
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| title |
Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_unstemmed |
Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_full |
Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_fullStr |
Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_full_unstemmed |
Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_short |
Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_sort |
overexpression of a hyperpolarization-activated cation current (<i>i</i><sub>h</sub>) channel gene modifies the firing activity of identified motor neurons in a small neural network |
| topic |
General Neuroscience |
| url |
http://dx.doi.org/10.1523/jneurosci.23-27-09059.2003 |
| publishDate |
2003 |
| physical |
9059-9067 |
| description |
<jats:p>The hyperpolarization-activated cation current (<jats:italic>I</jats:italic><jats:sub>h</jats:sub>) is widely distributed in excitable cells.<jats:italic>I</jats:italic><jats:sub>h</jats:sub>plays important roles in regulation of cellular excitability, rhythmic activity, and synaptic function. We previously showed that, in pyloric dilator (PD) neurons of the stomatogastric ganglion (STG) of spiny lobsters,<jats:italic>I</jats:italic><jats:sub>h</jats:sub>can be endogenously upregulated to compensate for artificial overexpression of the Shal transient potassium channel; this maintains normal firing properties of the neuron despite large increases in potassium current. To further explore the function of<jats:italic>I</jats:italic><jats:sub>h</jats:sub>in the pyloric network, we injected cRNA of<jats:italic>PAIH</jats:italic>, a lobster gene that encodes<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, into rhythmically active PD neurons. Overexpression of PAIH produced a fourfold increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, although with somewhat different biophysical properties than the endogenous current. Compared with the endogenous<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, the voltage for half-maximal activation of the PAIH-evoked current was depolarized by 10 mV, and its activation kinetics were significantly faster. This increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>did not affect the expression of<jats:italic>I</jats:italic><jats:sub>A</jats:sub>or other outward currents. Instead, it significantly altered the firing properties of the PD neurons. Increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>depolarized the minimum membrane potential of the cell, reduced the oscillation amplitude, decreased the time to the first spike, and increased the duty cycle and number of action potentials per burst. We used both dynamic-clamp experiments, injecting the modeled PAIH currents into PD cells in a functioning STG, and a theoretical model of a two-cell network to demonstrate that the increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>was sufficient to cause the observed changes in the PD activity.</jats:p> |
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| author | Zhang, Ying, Oliva, Ricardo, Gisselmann, Günter, Hatt, Hanns, Guckenheimer, John, Harris-Warrick, Ronald M. |
| author_facet | Zhang, Ying, Oliva, Ricardo, Gisselmann, Günter, Hatt, Hanns, Guckenheimer, John, Harris-Warrick, Ronald M., Zhang, Ying, Oliva, Ricardo, Gisselmann, Günter, Hatt, Hanns, Guckenheimer, John, Harris-Warrick, Ronald M. |
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| description | <jats:p>The hyperpolarization-activated cation current (<jats:italic>I</jats:italic><jats:sub>h</jats:sub>) is widely distributed in excitable cells.<jats:italic>I</jats:italic><jats:sub>h</jats:sub>plays important roles in regulation of cellular excitability, rhythmic activity, and synaptic function. We previously showed that, in pyloric dilator (PD) neurons of the stomatogastric ganglion (STG) of spiny lobsters,<jats:italic>I</jats:italic><jats:sub>h</jats:sub>can be endogenously upregulated to compensate for artificial overexpression of the Shal transient potassium channel; this maintains normal firing properties of the neuron despite large increases in potassium current. To further explore the function of<jats:italic>I</jats:italic><jats:sub>h</jats:sub>in the pyloric network, we injected cRNA of<jats:italic>PAIH</jats:italic>, a lobster gene that encodes<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, into rhythmically active PD neurons. Overexpression of PAIH produced a fourfold increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, although with somewhat different biophysical properties than the endogenous current. Compared with the endogenous<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, the voltage for half-maximal activation of the PAIH-evoked current was depolarized by 10 mV, and its activation kinetics were significantly faster. This increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>did not affect the expression of<jats:italic>I</jats:italic><jats:sub>A</jats:sub>or other outward currents. Instead, it significantly altered the firing properties of the PD neurons. Increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>depolarized the minimum membrane potential of the cell, reduced the oscillation amplitude, decreased the time to the first spike, and increased the duty cycle and number of action potentials per burst. We used both dynamic-clamp experiments, injecting the modeled PAIH currents into PD cells in a functioning STG, and a theoretical model of a two-cell network to demonstrate that the increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>was sufficient to cause the observed changes in the PD activity.</jats:p> |
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| match_str | zhang2003overexpressionofahyperpolarizationactivatedcationcurrentihchannelgenemodifiesthefiringactivityofidentifiedmotorneuronsinasmallneuralnetwork |
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| spelling | Zhang, Ying Oliva, Ricardo Gisselmann, Günter Hatt, Hanns Guckenheimer, John Harris-Warrick, Ronald M. 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.23-27-09059.2003 <jats:p>The hyperpolarization-activated cation current (<jats:italic>I</jats:italic><jats:sub>h</jats:sub>) is widely distributed in excitable cells.<jats:italic>I</jats:italic><jats:sub>h</jats:sub>plays important roles in regulation of cellular excitability, rhythmic activity, and synaptic function. We previously showed that, in pyloric dilator (PD) neurons of the stomatogastric ganglion (STG) of spiny lobsters,<jats:italic>I</jats:italic><jats:sub>h</jats:sub>can be endogenously upregulated to compensate for artificial overexpression of the Shal transient potassium channel; this maintains normal firing properties of the neuron despite large increases in potassium current. To further explore the function of<jats:italic>I</jats:italic><jats:sub>h</jats:sub>in the pyloric network, we injected cRNA of<jats:italic>PAIH</jats:italic>, a lobster gene that encodes<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, into rhythmically active PD neurons. Overexpression of PAIH produced a fourfold increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, although with somewhat different biophysical properties than the endogenous current. Compared with the endogenous<jats:italic>I</jats:italic><jats:sub>h</jats:sub>, the voltage for half-maximal activation of the PAIH-evoked current was depolarized by 10 mV, and its activation kinetics were significantly faster. This increase in<jats:italic>I</jats:italic><jats:sub>h</jats:sub>did not affect the expression of<jats:italic>I</jats:italic><jats:sub>A</jats:sub>or other outward currents. Instead, it significantly altered the firing properties of the PD neurons. Increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>depolarized the minimum membrane potential of the cell, reduced the oscillation amplitude, decreased the time to the first spike, and increased the duty cycle and number of action potentials per burst. We used both dynamic-clamp experiments, injecting the modeled PAIH currents into PD cells in a functioning STG, and a theoretical model of a two-cell network to demonstrate that the increased<jats:italic>I</jats:italic><jats:sub>h</jats:sub>was sufficient to cause the observed changes in the PD activity.</jats:p> Overexpression of a Hyperpolarization-Activated Cation Current (<i>I</i><sub>h</sub>) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network The Journal of Neuroscience |
| spellingShingle | Zhang, Ying, Oliva, Ricardo, Gisselmann, Günter, Hatt, Hanns, Guckenheimer, John, Harris-Warrick, Ronald M., The Journal of Neuroscience, Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network, General Neuroscience |
| title | Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_full | Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_fullStr | Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_full_unstemmed | Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_short | Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| title_sort | overexpression of a hyperpolarization-activated cation current (<i>i</i><sub>h</sub>) channel gene modifies the firing activity of identified motor neurons in a small neural network |
| title_unstemmed | Overexpression of a Hyperpolarization-Activated Cation Current (Ih) Channel Gene Modifies the Firing Activity of Identified Motor Neurons in a Small Neural Network |
| topic | General Neuroscience |
| url | http://dx.doi.org/10.1523/jneurosci.23-27-09059.2003 |