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Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2
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| Zeitschriftentitel: | International Journal of Food Engineering |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | International Journal of Food Engineering, 15, 2019, 1-2 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Walter de Gruyter GmbH
|
| Schlagwörter: |
| author_facet |
Zhao, Yuan Zhang, Shiyao Zhang, Xiaodong Pan, Li Bao, Nan Qin, Guixin Zhao, Yuan Zhang, Shiyao Zhang, Xiaodong Pan, Li Bao, Nan Qin, Guixin |
|---|---|
| author |
Zhao, Yuan Zhang, Shiyao Zhang, Xiaodong Pan, Li Bao, Nan Qin, Guixin |
| spellingShingle |
Zhao, Yuan Zhang, Shiyao Zhang, Xiaodong Pan, Li Bao, Nan Qin, Guixin International Journal of Food Engineering Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 Engineering (miscellaneous) Food Science Biotechnology |
| author_sort |
zhao, yuan |
| spelling |
Zhao, Yuan Zhang, Shiyao Zhang, Xiaodong Pan, Li Bao, Nan Qin, Guixin 1556-3758 2194-5764 Walter de Gruyter GmbH Engineering (miscellaneous) Food Science Biotechnology http://dx.doi.org/10.1515/ijfe-2018-0116 <jats:title>Abstract</jats:title> <jats:p>Dissecting the inhibited variation of allergen absorption could contribute to the development of novel therapeutic or preventive treatments for food/feed allergies. This study investigated the effects of fructooligosaccharide (FOS) on the absorption, intracellular accumulation of intact or hydrolysed β-conglycinin in porcine intestinal epithelial cells (IPEC-J2). As demonstrated by ELISA and immunoblotting, β-conglycinin was absorbed in a dose- and time-dependent manner (<jats:italic>p</jats:italic> < 0.05). Actually, β-conglycinin was easily transported and absorbed after enzymatic hydrolysis. Three peptides (52 kDa, 30 kDa and 25 kDa) were produced during transcellular absorption of intact or hydrolysed β-conglycinin. FOS inhibited the absorption of β-conglycinin, especially the 52 and 30 kDa peptides. The immunoreactive peptides derived from the 52, 35 or 22 kDa peptides were the substrings of the known epitopes determined by mass spectrometry and bioinformatic analyses. These results indicate that FOS can efficiently inhibit the absorption of 52 and 30 kDa peptides derived from β-conglycinin.</jats:p> Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 International Journal of Food Engineering |
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| title |
Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_unstemmed |
Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_full |
Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_fullStr |
Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_full_unstemmed |
Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_short |
Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_sort |
fructooligosaccharide inhibits the absorption of β-conglycinin (a major soybean allergen) in ipec-j2 |
| topic |
Engineering (miscellaneous) Food Science Biotechnology |
| url |
http://dx.doi.org/10.1515/ijfe-2018-0116 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:p>Dissecting the inhibited variation of allergen absorption could contribute to the development of novel therapeutic or preventive treatments for food/feed allergies. This study investigated the effects of fructooligosaccharide (FOS) on the absorption, intracellular accumulation of intact or hydrolysed β-conglycinin in porcine intestinal epithelial cells (IPEC-J2). As demonstrated by ELISA and immunoblotting, β-conglycinin was absorbed in a dose- and time-dependent manner (<jats:italic>p</jats:italic> < 0.05). Actually, β-conglycinin was easily transported and absorbed after enzymatic hydrolysis. Three peptides (52 kDa, 30 kDa and 25 kDa) were produced during transcellular absorption of intact or hydrolysed β-conglycinin. FOS inhibited the absorption of β-conglycinin, especially the 52 and 30 kDa peptides. The immunoreactive peptides derived from the 52, 35 or 22 kDa peptides were the substrings of the known epitopes determined by mass spectrometry and bioinformatic analyses. These results indicate that FOS can efficiently inhibit the absorption of 52 and 30 kDa peptides derived from β-conglycinin.</jats:p> |
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| author | Zhao, Yuan, Zhang, Shiyao, Zhang, Xiaodong, Pan, Li, Bao, Nan, Qin, Guixin |
| author_facet | Zhao, Yuan, Zhang, Shiyao, Zhang, Xiaodong, Pan, Li, Bao, Nan, Qin, Guixin, Zhao, Yuan, Zhang, Shiyao, Zhang, Xiaodong, Pan, Li, Bao, Nan, Qin, Guixin |
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| description | <jats:title>Abstract</jats:title> <jats:p>Dissecting the inhibited variation of allergen absorption could contribute to the development of novel therapeutic or preventive treatments for food/feed allergies. This study investigated the effects of fructooligosaccharide (FOS) on the absorption, intracellular accumulation of intact or hydrolysed β-conglycinin in porcine intestinal epithelial cells (IPEC-J2). As demonstrated by ELISA and immunoblotting, β-conglycinin was absorbed in a dose- and time-dependent manner (<jats:italic>p</jats:italic> < 0.05). Actually, β-conglycinin was easily transported and absorbed after enzymatic hydrolysis. Three peptides (52 kDa, 30 kDa and 25 kDa) were produced during transcellular absorption of intact or hydrolysed β-conglycinin. FOS inhibited the absorption of β-conglycinin, especially the 52 and 30 kDa peptides. The immunoreactive peptides derived from the 52, 35 or 22 kDa peptides were the substrings of the known epitopes determined by mass spectrometry and bioinformatic analyses. These results indicate that FOS can efficiently inhibit the absorption of 52 and 30 kDa peptides derived from β-conglycinin.</jats:p> |
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| spelling | Zhao, Yuan Zhang, Shiyao Zhang, Xiaodong Pan, Li Bao, Nan Qin, Guixin 1556-3758 2194-5764 Walter de Gruyter GmbH Engineering (miscellaneous) Food Science Biotechnology http://dx.doi.org/10.1515/ijfe-2018-0116 <jats:title>Abstract</jats:title> <jats:p>Dissecting the inhibited variation of allergen absorption could contribute to the development of novel therapeutic or preventive treatments for food/feed allergies. This study investigated the effects of fructooligosaccharide (FOS) on the absorption, intracellular accumulation of intact or hydrolysed β-conglycinin in porcine intestinal epithelial cells (IPEC-J2). As demonstrated by ELISA and immunoblotting, β-conglycinin was absorbed in a dose- and time-dependent manner (<jats:italic>p</jats:italic> < 0.05). Actually, β-conglycinin was easily transported and absorbed after enzymatic hydrolysis. Three peptides (52 kDa, 30 kDa and 25 kDa) were produced during transcellular absorption of intact or hydrolysed β-conglycinin. FOS inhibited the absorption of β-conglycinin, especially the 52 and 30 kDa peptides. The immunoreactive peptides derived from the 52, 35 or 22 kDa peptides were the substrings of the known epitopes determined by mass spectrometry and bioinformatic analyses. These results indicate that FOS can efficiently inhibit the absorption of 52 and 30 kDa peptides derived from β-conglycinin.</jats:p> Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 International Journal of Food Engineering |
| spellingShingle | Zhao, Yuan, Zhang, Shiyao, Zhang, Xiaodong, Pan, Li, Bao, Nan, Qin, Guixin, International Journal of Food Engineering, Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2, Engineering (miscellaneous), Food Science, Biotechnology |
| title | Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_full | Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_fullStr | Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_full_unstemmed | Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_short | Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| title_sort | fructooligosaccharide inhibits the absorption of β-conglycinin (a major soybean allergen) in ipec-j2 |
| title_unstemmed | Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2 |
| topic | Engineering (miscellaneous), Food Science, Biotechnology |
| url | http://dx.doi.org/10.1515/ijfe-2018-0116 |