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Hypothyroidism induces expression of the peptide transporter PEPT2
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| Zeitschriftentitel: | Biological Chemistry |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Biological Chemistry, 386, 2005, 8, S. 785-790 |
| Format: | E-Article |
| Sprache: | Unbestimmt |
| veröffentlicht: |
Walter de Gruyter GmbH
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| Schlagwörter: |
| author_facet |
Döring, Frank Schmitt, Roland Bernhardt, Wanja M. Klapper, Maja Bachmann, Sebastian Daniel, Hannelore Groneberg, David A. Döring, Frank Schmitt, Roland Bernhardt, Wanja M. Klapper, Maja Bachmann, Sebastian Daniel, Hannelore Groneberg, David A. |
|---|---|
| author |
Döring, Frank Schmitt, Roland Bernhardt, Wanja M. Klapper, Maja Bachmann, Sebastian Daniel, Hannelore Groneberg, David A. |
| spellingShingle |
Döring, Frank Schmitt, Roland Bernhardt, Wanja M. Klapper, Maja Bachmann, Sebastian Daniel, Hannelore Groneberg, David A. Biological Chemistry Hypothyroidism induces expression of the peptide transporter PEPT2 Clinical Biochemistry Molecular Biology Biochemistry |
| author_sort |
döring, frank |
| spelling |
Döring, Frank Schmitt, Roland Bernhardt, Wanja M. Klapper, Maja Bachmann, Sebastian Daniel, Hannelore Groneberg, David A. 1431-6730 1437-4315 Walter de Gruyter GmbH Clinical Biochemistry Molecular Biology Biochemistry http://dx.doi.org/10.1515/bc.2005.092 <jats:title>Abstract</jats:title> <jats:p>The kidney is a target organ for thyroid hormone action and a variety of renal transport processes are altered in response to impaired thyroid functions. To investigate the effect of thyroid hormone on the expression of the renal proximal tubular high-affinity-type H<jats:sup>+</jats:sup>-peptide cotransporter (PEPT2) in rats, hypothyroidism was induced in animals by administration of methimazole (0.05%) via drinking water. After 7 weeks of treatment, hypothyroidism was confirmed by determining serum free T<jats:sub>3</jats:sub> and free T<jats:sub>4</jats:sub> concentrations. Northern blotting was used to examine the expression of PEPT2 mRNA in kidney tissues from hypothyroid rats compared to control rats. Hypothyroidism resulted in an increased level of total renal PEPT2 mRNA (121.1±3.3% vs. control 100±2.8%; <jats:italic>p</jats:italic>=0.008). The mRNA results were confirmed by immuno-blotting, which demonstrated significantly increased protein levels (162% vs. control 100%; <jats:italic>p</jats:italic><0.01). Immunohistochemistry also revealed increased PEPT2 protein levels in the proximal tubules of treated compared to non-treated rats. In summary, PEPT2 is the first proximal tubule transporter protein that shows increased expression in states of hypothyreosis. As PEPT2 reabsorbs filtered di- and tripeptides and peptide-like drugs, the present findings may have important implications in nutritional amino acid homeostasis and for drug dynamics in states of altered thyroid function.</jats:p> Hypothyroidism induces expression of the peptide transporter PEPT2 Biological Chemistry |
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10.1515/bc.2005.092 |
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49 |
| title |
Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_unstemmed |
Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_full |
Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_fullStr |
Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_full_unstemmed |
Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_short |
Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_sort |
hypothyroidism induces expression of the peptide transporter pept2 |
| topic |
Clinical Biochemistry Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1515/bc.2005.092 |
| publishDate |
2005 |
| physical |
785-790 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>The kidney is a target organ for thyroid hormone action and a variety of renal transport processes are altered in response to impaired thyroid functions. To investigate the effect of thyroid hormone on the expression of the renal proximal tubular high-affinity-type H<jats:sup>+</jats:sup>-peptide cotransporter (PEPT2) in rats, hypothyroidism was induced in animals by administration of methimazole (0.05%) via drinking water. After 7 weeks of treatment, hypothyroidism was confirmed by determining serum free T<jats:sub>3</jats:sub> and free T<jats:sub>4</jats:sub> concentrations. Northern blotting was used to examine the expression of PEPT2 mRNA in kidney tissues from hypothyroid rats compared to control rats. Hypothyroidism resulted in an increased level of total renal PEPT2 mRNA (121.1±3.3% vs. control 100±2.8%; <jats:italic>p</jats:italic>=0.008). The mRNA results were confirmed by immuno-blotting, which demonstrated significantly increased protein levels (162% vs. control 100%; <jats:italic>p</jats:italic><0.01). Immunohistochemistry also revealed increased PEPT2 protein levels in the proximal tubules of treated compared to non-treated rats. In summary, PEPT2 is the first proximal tubule transporter protein that shows increased expression in states of hypothyreosis. As PEPT2 reabsorbs filtered di- and tripeptides and peptide-like drugs, the present findings may have important implications in nutritional amino acid homeostasis and for drug dynamics in states of altered thyroid function.</jats:p> |
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| author | Döring, Frank, Schmitt, Roland, Bernhardt, Wanja M., Klapper, Maja, Bachmann, Sebastian, Daniel, Hannelore, Groneberg, David A. |
| author_facet | Döring, Frank, Schmitt, Roland, Bernhardt, Wanja M., Klapper, Maja, Bachmann, Sebastian, Daniel, Hannelore, Groneberg, David A., Döring, Frank, Schmitt, Roland, Bernhardt, Wanja M., Klapper, Maja, Bachmann, Sebastian, Daniel, Hannelore, Groneberg, David A. |
| author_sort | döring, frank |
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| description | <jats:title>Abstract</jats:title> <jats:p>The kidney is a target organ for thyroid hormone action and a variety of renal transport processes are altered in response to impaired thyroid functions. To investigate the effect of thyroid hormone on the expression of the renal proximal tubular high-affinity-type H<jats:sup>+</jats:sup>-peptide cotransporter (PEPT2) in rats, hypothyroidism was induced in animals by administration of methimazole (0.05%) via drinking water. After 7 weeks of treatment, hypothyroidism was confirmed by determining serum free T<jats:sub>3</jats:sub> and free T<jats:sub>4</jats:sub> concentrations. Northern blotting was used to examine the expression of PEPT2 mRNA in kidney tissues from hypothyroid rats compared to control rats. Hypothyroidism resulted in an increased level of total renal PEPT2 mRNA (121.1±3.3% vs. control 100±2.8%; <jats:italic>p</jats:italic>=0.008). The mRNA results were confirmed by immuno-blotting, which demonstrated significantly increased protein levels (162% vs. control 100%; <jats:italic>p</jats:italic><0.01). Immunohistochemistry also revealed increased PEPT2 protein levels in the proximal tubules of treated compared to non-treated rats. In summary, PEPT2 is the first proximal tubule transporter protein that shows increased expression in states of hypothyreosis. As PEPT2 reabsorbs filtered di- and tripeptides and peptide-like drugs, the present findings may have important implications in nutritional amino acid homeostasis and for drug dynamics in states of altered thyroid function.</jats:p> |
| doi_str_mv | 10.1515/bc.2005.092 |
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| imprint | Walter de Gruyter GmbH, 2005 |
| imprint_str_mv | Walter de Gruyter GmbH, 2005 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| spelling | Döring, Frank Schmitt, Roland Bernhardt, Wanja M. Klapper, Maja Bachmann, Sebastian Daniel, Hannelore Groneberg, David A. 1431-6730 1437-4315 Walter de Gruyter GmbH Clinical Biochemistry Molecular Biology Biochemistry http://dx.doi.org/10.1515/bc.2005.092 <jats:title>Abstract</jats:title> <jats:p>The kidney is a target organ for thyroid hormone action and a variety of renal transport processes are altered in response to impaired thyroid functions. To investigate the effect of thyroid hormone on the expression of the renal proximal tubular high-affinity-type H<jats:sup>+</jats:sup>-peptide cotransporter (PEPT2) in rats, hypothyroidism was induced in animals by administration of methimazole (0.05%) via drinking water. After 7 weeks of treatment, hypothyroidism was confirmed by determining serum free T<jats:sub>3</jats:sub> and free T<jats:sub>4</jats:sub> concentrations. Northern blotting was used to examine the expression of PEPT2 mRNA in kidney tissues from hypothyroid rats compared to control rats. Hypothyroidism resulted in an increased level of total renal PEPT2 mRNA (121.1±3.3% vs. control 100±2.8%; <jats:italic>p</jats:italic>=0.008). The mRNA results were confirmed by immuno-blotting, which demonstrated significantly increased protein levels (162% vs. control 100%; <jats:italic>p</jats:italic><0.01). Immunohistochemistry also revealed increased PEPT2 protein levels in the proximal tubules of treated compared to non-treated rats. In summary, PEPT2 is the first proximal tubule transporter protein that shows increased expression in states of hypothyreosis. As PEPT2 reabsorbs filtered di- and tripeptides and peptide-like drugs, the present findings may have important implications in nutritional amino acid homeostasis and for drug dynamics in states of altered thyroid function.</jats:p> Hypothyroidism induces expression of the peptide transporter PEPT2 Biological Chemistry |
| spellingShingle | Döring, Frank, Schmitt, Roland, Bernhardt, Wanja M., Klapper, Maja, Bachmann, Sebastian, Daniel, Hannelore, Groneberg, David A., Biological Chemistry, Hypothyroidism induces expression of the peptide transporter PEPT2, Clinical Biochemistry, Molecular Biology, Biochemistry |
| title | Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_full | Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_fullStr | Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_full_unstemmed | Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_short | Hypothyroidism induces expression of the peptide transporter PEPT2 |
| title_sort | hypothyroidism induces expression of the peptide transporter pept2 |
| title_unstemmed | Hypothyroidism induces expression of the peptide transporter PEPT2 |
| topic | Clinical Biochemistry, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1515/bc.2005.092 |