Eintrag weiter verarbeiten
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques
Gespeichert in:
| Zeitschriftentitel: | Zeitschrift für Naturforschung C |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Zeitschrift für Naturforschung C, 28, 1973, 11-12, S. 722-732 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Walter de Gruyter GmbH
|
| Schlagwörter: |
| author_facet |
Stegemann, H Francksen, H Macko, V Stegemann, H Francksen, H Macko, V |
|---|---|
| author |
Stegemann, H Francksen, H Macko, V |
| spellingShingle |
Stegemann, H Francksen, H Macko, V Zeitschrift für Naturforschung C Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques General Biochemistry, Genetics and Molecular Biology |
| author_sort |
stegemann, h |
| spelling |
Stegemann, H Francksen, H Macko, V 1865-7125 0939-5075 Walter de Gruyter GmbH General Biochemistry, Genetics and Molecular Biology http://dx.doi.org/10.1515/znc-1973-11-1213 <jats:title>Abstract</jats:title> <jats:p> The proteins of mature potato tubers can be used for variety identification. Their pattern after electrophoresis or focusing are genetically determined. Various growth regulators applied during the season had no influence. Molecular weights of the protein subunits are uniform and decrease with age of the tuber, often due to depolymerization. Some subunits are linked by S -S-bridges. A proteid characteristic for immature tubers has been discovered. Part of the diversity in charge distribution is based on different degree of amidation; treatment at pH 10 changes the variety-dependent pattern to a picture similar in all varieties. The latter is also true for proteins from immature tubers. Focusing followed by SDS-incubation (with and without ME) and SDS-gelelectro-phoresis revealed that the main proteins which were different in charge had common MW and may derive from a parent protein.</jats:p> <jats:p>The tuber proteins and especially some of the peroxidases are quite resistant to SDS-attack. Only prolonged incubation (50 °C, 40 hours) with SDS brings about digestibility. A borate buffer, pH 7.9 has been used, making possible the electrophoresis of undiluted, unfractionated sap or other crude mixtures of proteins. Some new versions of two-dimensional techniques with PAA as a carrier are applied in order to differentiate between charge, shift of charge, and MW</jats:p> Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques Zeitschrift für Naturforschung C |
| doi_str_mv |
10.1515/znc-1973-11-1213 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUxNS96bmMtMTk3My0xMS0xMjEz |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUxNS96bmMtMTk3My0xMS0xMjEz |
| institution |
DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
Walter de Gruyter GmbH, 1973 |
| imprint_str_mv |
Walter de Gruyter GmbH, 1973 |
| issn |
1865-7125 0939-5075 |
| issn_str_mv |
1865-7125 0939-5075 |
| language |
English |
| mega_collection |
Walter de Gruyter GmbH (CrossRef) |
| match_str |
stegemann1973potatoproteinsgeneticandphysiologicalchangesevaluatedbyoneandtwodimensionalpaageltechniques |
| publishDateSort |
1973 |
| publisher |
Walter de Gruyter GmbH |
| recordtype |
ai |
| record_format |
ai |
| series |
Zeitschrift für Naturforschung C |
| source_id |
49 |
| title |
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_unstemmed |
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_full |
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_fullStr |
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_full_unstemmed |
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_short |
Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_sort |
potato proteins: genetic and physiological changes, evaluated by one-and two-dimensional paa-gel-techniques |
| topic |
General Biochemistry, Genetics and Molecular Biology |
| url |
http://dx.doi.org/10.1515/znc-1973-11-1213 |
| publishDate |
1973 |
| physical |
722-732 |
| description |
<jats:title>Abstract</jats:title>
<jats:p> The proteins of mature potato tubers can be used for variety identification. Their pattern after electrophoresis or focusing are genetically determined. Various growth regulators applied during the season had no influence. Molecular weights of the protein subunits are uniform and decrease with age of the tuber, often due to depolymerization. Some subunits are linked by S -S-bridges. A proteid characteristic for immature tubers has been discovered. Part of the diversity in charge distribution is based on different degree of amidation; treatment at pH 10 changes the variety-dependent pattern to a picture similar in all varieties. The latter is also true for proteins from immature tubers. Focusing followed by SDS-incubation (with and without ME) and SDS-gelelectro-phoresis revealed that the main proteins which were different in charge had common MW and may derive from a parent protein.</jats:p>
<jats:p>The tuber proteins and especially some of the peroxidases are quite resistant to SDS-attack. Only prolonged incubation (50 °C, 40 hours) with SDS brings about digestibility. A borate buffer, pH 7.9 has been used, making possible the electrophoresis of undiluted, unfractionated sap or other crude mixtures of proteins. Some new versions of two-dimensional techniques with PAA as a carrier are applied in order to differentiate between charge, shift of charge, and MW</jats:p> |
| container_issue |
11-12 |
| container_start_page |
722 |
| container_title |
Zeitschrift für Naturforschung C |
| container_volume |
28 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792339267980099588 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:45:25.872Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Potato+Proteins%3A+Genetic+and+Physiological+Changes%2C+Evaluated+by+One-and+Two-dimensional+PAA-Gel-techniques&rft.date=1973-12-01&genre=article&issn=0939-5075&volume=28&issue=11-12&spage=722&epage=732&pages=722-732&jtitle=Zeitschrift+f%C3%BCr+Naturforschung+C&atitle=Potato+Proteins%3A+Genetic+and+Physiological+Changes%2C+Evaluated+by+One-and+Two-dimensional+PAA-Gel-techniques&aulast=Macko&aufirst=V&rft_id=info%3Adoi%2F10.1515%2Fznc-1973-11-1213&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792339267980099588 |
| author | Stegemann, H, Francksen, H, Macko, V |
| author_facet | Stegemann, H, Francksen, H, Macko, V, Stegemann, H, Francksen, H, Macko, V |
| author_sort | stegemann, h |
| container_issue | 11-12 |
| container_start_page | 722 |
| container_title | Zeitschrift für Naturforschung C |
| container_volume | 28 |
| description | <jats:title>Abstract</jats:title> <jats:p> The proteins of mature potato tubers can be used for variety identification. Their pattern after electrophoresis or focusing are genetically determined. Various growth regulators applied during the season had no influence. Molecular weights of the protein subunits are uniform and decrease with age of the tuber, often due to depolymerization. Some subunits are linked by S -S-bridges. A proteid characteristic for immature tubers has been discovered. Part of the diversity in charge distribution is based on different degree of amidation; treatment at pH 10 changes the variety-dependent pattern to a picture similar in all varieties. The latter is also true for proteins from immature tubers. Focusing followed by SDS-incubation (with and without ME) and SDS-gelelectro-phoresis revealed that the main proteins which were different in charge had common MW and may derive from a parent protein.</jats:p> <jats:p>The tuber proteins and especially some of the peroxidases are quite resistant to SDS-attack. Only prolonged incubation (50 °C, 40 hours) with SDS brings about digestibility. A borate buffer, pH 7.9 has been used, making possible the electrophoresis of undiluted, unfractionated sap or other crude mixtures of proteins. Some new versions of two-dimensional techniques with PAA as a carrier are applied in order to differentiate between charge, shift of charge, and MW</jats:p> |
| doi_str_mv | 10.1515/znc-1973-11-1213 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUxNS96bmMtMTk3My0xMS0xMjEz |
| imprint | Walter de Gruyter GmbH, 1973 |
| imprint_str_mv | Walter de Gruyter GmbH, 1973 |
| institution | DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 1865-7125, 0939-5075 |
| issn_str_mv | 1865-7125, 0939-5075 |
| language | English |
| last_indexed | 2024-03-01T15:45:25.872Z |
| match_str | stegemann1973potatoproteinsgeneticandphysiologicalchangesevaluatedbyoneandtwodimensionalpaageltechniques |
| mega_collection | Walter de Gruyter GmbH (CrossRef) |
| physical | 722-732 |
| publishDate | 1973 |
| publishDateSort | 1973 |
| publisher | Walter de Gruyter GmbH |
| record_format | ai |
| recordtype | ai |
| series | Zeitschrift für Naturforschung C |
| source_id | 49 |
| spelling | Stegemann, H Francksen, H Macko, V 1865-7125 0939-5075 Walter de Gruyter GmbH General Biochemistry, Genetics and Molecular Biology http://dx.doi.org/10.1515/znc-1973-11-1213 <jats:title>Abstract</jats:title> <jats:p> The proteins of mature potato tubers can be used for variety identification. Their pattern after electrophoresis or focusing are genetically determined. Various growth regulators applied during the season had no influence. Molecular weights of the protein subunits are uniform and decrease with age of the tuber, often due to depolymerization. Some subunits are linked by S -S-bridges. A proteid characteristic for immature tubers has been discovered. Part of the diversity in charge distribution is based on different degree of amidation; treatment at pH 10 changes the variety-dependent pattern to a picture similar in all varieties. The latter is also true for proteins from immature tubers. Focusing followed by SDS-incubation (with and without ME) and SDS-gelelectro-phoresis revealed that the main proteins which were different in charge had common MW and may derive from a parent protein.</jats:p> <jats:p>The tuber proteins and especially some of the peroxidases are quite resistant to SDS-attack. Only prolonged incubation (50 °C, 40 hours) with SDS brings about digestibility. A borate buffer, pH 7.9 has been used, making possible the electrophoresis of undiluted, unfractionated sap or other crude mixtures of proteins. Some new versions of two-dimensional techniques with PAA as a carrier are applied in order to differentiate between charge, shift of charge, and MW</jats:p> Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques Zeitschrift für Naturforschung C |
| spellingShingle | Stegemann, H, Francksen, H, Macko, V, Zeitschrift für Naturforschung C, Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques, General Biochemistry, Genetics and Molecular Biology |
| title | Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_full | Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_fullStr | Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_full_unstemmed | Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_short | Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| title_sort | potato proteins: genetic and physiological changes, evaluated by one-and two-dimensional paa-gel-techniques |
| title_unstemmed | Potato Proteins: Genetic and Physiological Changes, Evaluated by One-and Two-dimensional PAA-Gel-techniques |
| topic | General Biochemistry, Genetics and Molecular Biology |
| url | http://dx.doi.org/10.1515/znc-1973-11-1213 |