Eintrag weiter verarbeiten
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands
Gespeichert in:
| Zeitschriftentitel: | Journal of Histochemistry & Cytochemistry |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Histochemistry & Cytochemistry, 65, 2017, 5, S. 261-272 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Gómez-Santos, Laura Alonso, Edurne Díaz-Flores, Lucio Madrid, Juan F. Sáez, Francisco J. Gómez-Santos, Laura Alonso, Edurne Díaz-Flores, Lucio Madrid, Juan F. Sáez, Francisco J. |
|---|---|
| author |
Gómez-Santos, Laura Alonso, Edurne Díaz-Flores, Lucio Madrid, Juan F. Sáez, Francisco J. |
| spellingShingle |
Gómez-Santos, Laura Alonso, Edurne Díaz-Flores, Lucio Madrid, Juan F. Sáez, Francisco J. Journal of Histochemistry & Cytochemistry Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands Histology Anatomy |
| author_sort |
gómez-santos, laura |
| spelling |
Gómez-Santos, Laura Alonso, Edurne Díaz-Flores, Lucio Madrid, Juan F. Sáez, Francisco J. 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1369/0022155417694871 <jats:p> Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid. The lectins from Helix pomatia, Dolichos biflorus (DBA), Glycine max (soybean), Maclura pomifera, Arachis hypogaea (peanut), Bandeiraea simplicifolia (lectin I-B<jats:sub>4</jats:sub>), and Datura stramonium showed a different glycan expression in the parietal cells throughout the gastric gland. This difference supports that parietal cells undergo a maturation/degeneration process while the cells descend along the gland. The role of DBA as a marker of parietal cells previously reported should be taken with caution because these cells showed different reactivity for the lectin, ranging from negative to strong labeling. </jats:p> Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands Journal of Histochemistry & Cytochemistry |
| doi_str_mv |
10.1369/0022155417694871 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTM2OS8wMDIyMTU1NDE3Njk0ODcx |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTM2OS8wMDIyMTU1NDE3Njk0ODcx |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
SAGE Publications, 2017 |
| imprint_str_mv |
SAGE Publications, 2017 |
| issn |
0022-1554 1551-5044 |
| issn_str_mv |
0022-1554 1551-5044 |
| language |
English |
| mega_collection |
SAGE Publications (CrossRef) |
| match_str |
gomezsantos2017characterizationbylectinhistochemistryoftwosubpopulationsofparietalcellsintheratgastricglands |
| publishDateSort |
2017 |
| publisher |
SAGE Publications |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Histochemistry & Cytochemistry |
| source_id |
49 |
| title |
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_unstemmed |
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_full |
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_fullStr |
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_full_unstemmed |
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_short |
Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_sort |
characterization by lectin histochemistry of two subpopulations of parietal cells in the rat gastric glands |
| topic |
Histology Anatomy |
| url |
http://dx.doi.org/10.1369/0022155417694871 |
| publishDate |
2017 |
| physical |
261-272 |
| description |
<jats:p> Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid. The lectins from Helix pomatia, Dolichos biflorus (DBA), Glycine max (soybean), Maclura pomifera, Arachis hypogaea (peanut), Bandeiraea simplicifolia (lectin I-B<jats:sub>4</jats:sub>), and Datura stramonium showed a different glycan expression in the parietal cells throughout the gastric gland. This difference supports that parietal cells undergo a maturation/degeneration process while the cells descend along the gland. The role of DBA as a marker of parietal cells previously reported should be taken with caution because these cells showed different reactivity for the lectin, ranging from negative to strong labeling. </jats:p> |
| container_issue |
5 |
| container_start_page |
261 |
| container_title |
Journal of Histochemistry & Cytochemistry |
| container_volume |
65 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792332687017508867 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:00:49.862Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Characterization+by+Lectin+Histochemistry+of+Two+Subpopulations+of+Parietal+Cells+in+the+Rat+Gastric+Glands&rft.date=2017-05-01&genre=article&issn=1551-5044&volume=65&issue=5&spage=261&epage=272&pages=261-272&jtitle=Journal+of+Histochemistry+%26+Cytochemistry&atitle=Characterization+by+Lectin+Histochemistry+of+Two+Subpopulations+of+Parietal+Cells+in+the+Rat+Gastric+Glands&aulast=S%C3%A1ez&aufirst=Francisco+J.&rft_id=info%3Adoi%2F10.1369%2F0022155417694871&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792332687017508867 |
| author | Gómez-Santos, Laura, Alonso, Edurne, Díaz-Flores, Lucio, Madrid, Juan F., Sáez, Francisco J. |
| author_facet | Gómez-Santos, Laura, Alonso, Edurne, Díaz-Flores, Lucio, Madrid, Juan F., Sáez, Francisco J., Gómez-Santos, Laura, Alonso, Edurne, Díaz-Flores, Lucio, Madrid, Juan F., Sáez, Francisco J. |
| author_sort | gómez-santos, laura |
| container_issue | 5 |
| container_start_page | 261 |
| container_title | Journal of Histochemistry & Cytochemistry |
| container_volume | 65 |
| description | <jats:p> Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid. The lectins from Helix pomatia, Dolichos biflorus (DBA), Glycine max (soybean), Maclura pomifera, Arachis hypogaea (peanut), Bandeiraea simplicifolia (lectin I-B<jats:sub>4</jats:sub>), and Datura stramonium showed a different glycan expression in the parietal cells throughout the gastric gland. This difference supports that parietal cells undergo a maturation/degeneration process while the cells descend along the gland. The role of DBA as a marker of parietal cells previously reported should be taken with caution because these cells showed different reactivity for the lectin, ranging from negative to strong labeling. </jats:p> |
| doi_str_mv | 10.1369/0022155417694871 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie, Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTM2OS8wMDIyMTU1NDE3Njk0ODcx |
| imprint | SAGE Publications, 2017 |
| imprint_str_mv | SAGE Publications, 2017 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0022-1554, 1551-5044 |
| issn_str_mv | 0022-1554, 1551-5044 |
| language | English |
| last_indexed | 2024-03-01T14:00:49.862Z |
| match_str | gomezsantos2017characterizationbylectinhistochemistryoftwosubpopulationsofparietalcellsintheratgastricglands |
| mega_collection | SAGE Publications (CrossRef) |
| physical | 261-272 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | SAGE Publications |
| record_format | ai |
| recordtype | ai |
| series | Journal of Histochemistry & Cytochemistry |
| source_id | 49 |
| spelling | Gómez-Santos, Laura Alonso, Edurne Díaz-Flores, Lucio Madrid, Juan F. Sáez, Francisco J. 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1369/0022155417694871 <jats:p> Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid. The lectins from Helix pomatia, Dolichos biflorus (DBA), Glycine max (soybean), Maclura pomifera, Arachis hypogaea (peanut), Bandeiraea simplicifolia (lectin I-B<jats:sub>4</jats:sub>), and Datura stramonium showed a different glycan expression in the parietal cells throughout the gastric gland. This difference supports that parietal cells undergo a maturation/degeneration process while the cells descend along the gland. The role of DBA as a marker of parietal cells previously reported should be taken with caution because these cells showed different reactivity for the lectin, ranging from negative to strong labeling. </jats:p> Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands Journal of Histochemistry & Cytochemistry |
| spellingShingle | Gómez-Santos, Laura, Alonso, Edurne, Díaz-Flores, Lucio, Madrid, Juan F., Sáez, Francisco J., Journal of Histochemistry & Cytochemistry, Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands, Histology, Anatomy |
| title | Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_full | Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_fullStr | Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_full_unstemmed | Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_short | Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| title_sort | characterization by lectin histochemistry of two subpopulations of parietal cells in the rat gastric glands |
| title_unstemmed | Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands |
| topic | Histology, Anatomy |
| url | http://dx.doi.org/10.1369/0022155417694871 |