Eintrag weiter verarbeiten
Analytic Response Curves of Clinical Breast Cancer IHC Tests
Gespeichert in:
| Zeitschriftentitel: | Journal of Histochemistry & Cytochemistry |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Histochemistry & Cytochemistry, 65, 2017, 5, S. 273-283 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Vani, Kodela Sompuram, Seshi R. Schaedle, Anika K. Balasubramanian, Anuradha Bogen, Steven A. Vani, Kodela Sompuram, Seshi R. Schaedle, Anika K. Balasubramanian, Anuradha Bogen, Steven A. |
|---|---|
| author |
Vani, Kodela Sompuram, Seshi R. Schaedle, Anika K. Balasubramanian, Anuradha Bogen, Steven A. |
| spellingShingle |
Vani, Kodela Sompuram, Seshi R. Schaedle, Anika K. Balasubramanian, Anuradha Bogen, Steven A. Journal of Histochemistry & Cytochemistry Analytic Response Curves of Clinical Breast Cancer IHC Tests Histology Anatomy |
| author_sort |
vani, kodela |
| spelling |
Vani, Kodela Sompuram, Seshi R. Schaedle, Anika K. Balasubramanian, Anuradha Bogen, Steven A. 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1369/0022155417694869 <jats:p> An important limitation in the field of immunohistochemistry (IHC) is the inability to correlate stain intensity with specific analyte concentrations. Clinical immunohistochemical tests are not described in terms of analytic response curves, namely, the analyte concentrations in a tissue sample at which an immunohistochemical stain (1) is first visible, (2) increases in proportion to the analyte concentration, and (3) ultimately approaches a maximum color intensity. Using a new immunostaining tool ( IHControls), we measured the analytic response curves of the major clinical immunohistochemical tests for human epidermal growth factor receptor type II (HER-2), estrogen receptor (ER), and progesterone receptor (PR). The IHControls comprise the analytes HER-2, ER, and PR at approximately log concentration intervals across the range of biological expression, from 100 to 1,000,000 molecules per test microbead. We stained IHControls of various concentrations using instruments, reagents, and protocols from three major IHC vendors. Stain intensity at each analyte concentration was measured, thereby generating an analytic response curve. We learned that for HER-2 and PR, there is significant variability in test results between clinical kits for samples with analyte concentrations of approximately 10<jats:sup>4</jats:sup> molecules/microbead. We propose that the characterization of immunostains is an important step toward standardization. </jats:p> Analytic Response Curves of Clinical Breast Cancer IHC Tests Journal of Histochemistry & Cytochemistry |
| doi_str_mv |
10.1369/0022155417694869 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTM2OS8wMDIyMTU1NDE3Njk0ODY5 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTM2OS8wMDIyMTU1NDE3Njk0ODY5 |
| institution |
DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 |
| imprint |
SAGE Publications, 2017 |
| imprint_str_mv |
SAGE Publications, 2017 |
| issn |
1551-5044 0022-1554 |
| issn_str_mv |
1551-5044 0022-1554 |
| language |
English |
| mega_collection |
SAGE Publications (CrossRef) |
| match_str |
vani2017analyticresponsecurvesofclinicalbreastcancerihctests |
| publishDateSort |
2017 |
| publisher |
SAGE Publications |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Histochemistry & Cytochemistry |
| source_id |
49 |
| title |
Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_unstemmed |
Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_full |
Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_fullStr |
Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_full_unstemmed |
Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_short |
Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_sort |
analytic response curves of clinical breast cancer ihc tests |
| topic |
Histology Anatomy |
| url |
http://dx.doi.org/10.1369/0022155417694869 |
| publishDate |
2017 |
| physical |
273-283 |
| description |
<jats:p> An important limitation in the field of immunohistochemistry (IHC) is the inability to correlate stain intensity with specific analyte concentrations. Clinical immunohistochemical tests are not described in terms of analytic response curves, namely, the analyte concentrations in a tissue sample at which an immunohistochemical stain (1) is first visible, (2) increases in proportion to the analyte concentration, and (3) ultimately approaches a maximum color intensity. Using a new immunostaining tool ( IHControls), we measured the analytic response curves of the major clinical immunohistochemical tests for human epidermal growth factor receptor type II (HER-2), estrogen receptor (ER), and progesterone receptor (PR). The IHControls comprise the analytes HER-2, ER, and PR at approximately log concentration intervals across the range of biological expression, from 100 to 1,000,000 molecules per test microbead. We stained IHControls of various concentrations using instruments, reagents, and protocols from three major IHC vendors. Stain intensity at each analyte concentration was measured, thereby generating an analytic response curve. We learned that for HER-2 and PR, there is significant variability in test results between clinical kits for samples with analyte concentrations of approximately 10<jats:sup>4</jats:sup> molecules/microbead. We propose that the characterization of immunostains is an important step toward standardization. </jats:p> |
| container_issue |
5 |
| container_start_page |
273 |
| container_title |
Journal of Histochemistry & Cytochemistry |
| container_volume |
65 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792334382066827264 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:27:45.803Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Analytic+Response+Curves+of+Clinical+Breast+Cancer+IHC+Tests&rft.date=2017-05-01&genre=article&issn=1551-5044&volume=65&issue=5&spage=273&epage=283&pages=273-283&jtitle=Journal+of+Histochemistry+%26+Cytochemistry&atitle=Analytic+Response+Curves+of+Clinical+Breast+Cancer+IHC+Tests&aulast=Bogen&aufirst=Steven+A.&rft_id=info%3Adoi%2F10.1369%2F0022155417694869&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792334382066827264 |
| author | Vani, Kodela, Sompuram, Seshi R., Schaedle, Anika K., Balasubramanian, Anuradha, Bogen, Steven A. |
| author_facet | Vani, Kodela, Sompuram, Seshi R., Schaedle, Anika K., Balasubramanian, Anuradha, Bogen, Steven A., Vani, Kodela, Sompuram, Seshi R., Schaedle, Anika K., Balasubramanian, Anuradha, Bogen, Steven A. |
| author_sort | vani, kodela |
| container_issue | 5 |
| container_start_page | 273 |
| container_title | Journal of Histochemistry & Cytochemistry |
| container_volume | 65 |
| description | <jats:p> An important limitation in the field of immunohistochemistry (IHC) is the inability to correlate stain intensity with specific analyte concentrations. Clinical immunohistochemical tests are not described in terms of analytic response curves, namely, the analyte concentrations in a tissue sample at which an immunohistochemical stain (1) is first visible, (2) increases in proportion to the analyte concentration, and (3) ultimately approaches a maximum color intensity. Using a new immunostaining tool ( IHControls), we measured the analytic response curves of the major clinical immunohistochemical tests for human epidermal growth factor receptor type II (HER-2), estrogen receptor (ER), and progesterone receptor (PR). The IHControls comprise the analytes HER-2, ER, and PR at approximately log concentration intervals across the range of biological expression, from 100 to 1,000,000 molecules per test microbead. We stained IHControls of various concentrations using instruments, reagents, and protocols from three major IHC vendors. Stain intensity at each analyte concentration was measured, thereby generating an analytic response curve. We learned that for HER-2 and PR, there is significant variability in test results between clinical kits for samples with analyte concentrations of approximately 10<jats:sup>4</jats:sup> molecules/microbead. We propose that the characterization of immunostains is an important step toward standardization. </jats:p> |
| doi_str_mv | 10.1369/0022155417694869 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie, Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTM2OS8wMDIyMTU1NDE3Njk0ODY5 |
| imprint | SAGE Publications, 2017 |
| imprint_str_mv | SAGE Publications, 2017 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 1551-5044, 0022-1554 |
| issn_str_mv | 1551-5044, 0022-1554 |
| language | English |
| last_indexed | 2024-03-01T14:27:45.803Z |
| match_str | vani2017analyticresponsecurvesofclinicalbreastcancerihctests |
| mega_collection | SAGE Publications (CrossRef) |
| physical | 273-283 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | SAGE Publications |
| record_format | ai |
| recordtype | ai |
| series | Journal of Histochemistry & Cytochemistry |
| source_id | 49 |
| spelling | Vani, Kodela Sompuram, Seshi R. Schaedle, Anika K. Balasubramanian, Anuradha Bogen, Steven A. 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1369/0022155417694869 <jats:p> An important limitation in the field of immunohistochemistry (IHC) is the inability to correlate stain intensity with specific analyte concentrations. Clinical immunohistochemical tests are not described in terms of analytic response curves, namely, the analyte concentrations in a tissue sample at which an immunohistochemical stain (1) is first visible, (2) increases in proportion to the analyte concentration, and (3) ultimately approaches a maximum color intensity. Using a new immunostaining tool ( IHControls), we measured the analytic response curves of the major clinical immunohistochemical tests for human epidermal growth factor receptor type II (HER-2), estrogen receptor (ER), and progesterone receptor (PR). The IHControls comprise the analytes HER-2, ER, and PR at approximately log concentration intervals across the range of biological expression, from 100 to 1,000,000 molecules per test microbead. We stained IHControls of various concentrations using instruments, reagents, and protocols from three major IHC vendors. Stain intensity at each analyte concentration was measured, thereby generating an analytic response curve. We learned that for HER-2 and PR, there is significant variability in test results between clinical kits for samples with analyte concentrations of approximately 10<jats:sup>4</jats:sup> molecules/microbead. We propose that the characterization of immunostains is an important step toward standardization. </jats:p> Analytic Response Curves of Clinical Breast Cancer IHC Tests Journal of Histochemistry & Cytochemistry |
| spellingShingle | Vani, Kodela, Sompuram, Seshi R., Schaedle, Anika K., Balasubramanian, Anuradha, Bogen, Steven A., Journal of Histochemistry & Cytochemistry, Analytic Response Curves of Clinical Breast Cancer IHC Tests, Histology, Anatomy |
| title | Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_full | Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_fullStr | Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_full_unstemmed | Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_short | Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| title_sort | analytic response curves of clinical breast cancer ihc tests |
| title_unstemmed | Analytic Response Curves of Clinical Breast Cancer IHC Tests |
| topic | Histology, Anatomy |
| url | http://dx.doi.org/10.1369/0022155417694869 |