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Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation
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| Zeitschriftentitel: | RNA |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | RNA, 9, 2003, 5, S. 599-606 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
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| Schlagwörter: |
| author_facet |
KIM, YOON KI LEE, SONG HEE KIM, CHON SAENG SEOL, SU KYOUNG JANG, SUNG KEY KIM, YOON KI LEE, SONG HEE KIM, CHON SAENG SEOL, SU KYOUNG JANG, SUNG KEY |
|---|---|
| author |
KIM, YOON KI LEE, SONG HEE KIM, CHON SAENG SEOL, SU KYOUNG JANG, SUNG KEY |
| spellingShingle |
KIM, YOON KI LEE, SONG HEE KIM, CHON SAENG SEOL, SU KYOUNG JANG, SUNG KEY RNA Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation Molecular Biology |
| author_sort |
kim, yoon ki |
| spelling |
KIM, YOON KI LEE, SONG HEE KIM, CHON SAENG SEOL, SU KYOUNG JANG, SUNG KEY 1355-8382 1469-9001 Cold Spring Harbor Laboratory Molecular Biology http://dx.doi.org/10.1261/rna.2185603 <jats:p>Hepatitis C virus (HCV) is a positive-sense RNA virus ∼9600 bases long. An internal ribosomal entry site (IRES) spans the 5′ nontranslated region, which is the most conserved and highly structured region of the HCV genome. In this study, we demonstrate that nucleotides 428–442 of the HCV core-coding sequence anneal to nucleotides 24–38 of the 5′NTR, and that this RNA–RNA interaction modulates IRES-dependent translation in rabbit reticulocyte lysate and in HepG2 cells. The inclusion of the core-coding sequence (nucleotides 428–442) significantly suppressed the translational efficiency directed by HCV IRES in dicistronic reporter systems, and this suppression was relieved by site-directed mutations that blocked the long-range interaction between nucleotides 24–38 and 428–442. These findings suggest that the long-range interaction between the HCV 5′NTR and the core-coding nucleotide sequence down-regulate cap-independent translation via HCV IRES. The modulation of protein synthesis by long-range RNA–RNA interaction may play a role in the regulation of viral gene expression.</jats:p> Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation RNA |
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Cold Spring Harbor Laboratory, 2003 |
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Cold Spring Harbor Laboratory, 2003 |
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| title |
Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_unstemmed |
Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_full |
Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_fullStr |
Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_full_unstemmed |
Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_short |
Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_sort |
long-range rna–rna interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis c virus modulates the ires-dependent translation |
| topic |
Molecular Biology |
| url |
http://dx.doi.org/10.1261/rna.2185603 |
| publishDate |
2003 |
| physical |
599-606 |
| description |
<jats:p>Hepatitis C virus (HCV) is a positive-sense RNA virus ∼9600 bases long. An internal ribosomal entry site (IRES) spans the 5′ nontranslated region, which is the most conserved and highly structured region of the HCV genome. In this study, we demonstrate that nucleotides 428–442 of the HCV core-coding sequence anneal to nucleotides 24–38 of the 5′NTR, and that this RNA–RNA interaction modulates IRES-dependent translation in rabbit reticulocyte lysate and in HepG2 cells. The inclusion of the core-coding sequence (nucleotides 428–442) significantly suppressed the translational efficiency directed by HCV IRES in dicistronic reporter systems, and this suppression was relieved by site-directed mutations that blocked the long-range interaction between nucleotides 24–38 and 428–442. These findings suggest that the long-range interaction between the HCV 5′NTR and the core-coding nucleotide sequence down-regulate cap-independent translation via HCV IRES. The modulation of protein synthesis by long-range RNA–RNA interaction may play a role in the regulation of viral gene expression.</jats:p> |
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| author | KIM, YOON KI, LEE, SONG HEE, KIM, CHON SAENG, SEOL, SU KYOUNG, JANG, SUNG KEY |
| author_facet | KIM, YOON KI, LEE, SONG HEE, KIM, CHON SAENG, SEOL, SU KYOUNG, JANG, SUNG KEY, KIM, YOON KI, LEE, SONG HEE, KIM, CHON SAENG, SEOL, SU KYOUNG, JANG, SUNG KEY |
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| description | <jats:p>Hepatitis C virus (HCV) is a positive-sense RNA virus ∼9600 bases long. An internal ribosomal entry site (IRES) spans the 5′ nontranslated region, which is the most conserved and highly structured region of the HCV genome. In this study, we demonstrate that nucleotides 428–442 of the HCV core-coding sequence anneal to nucleotides 24–38 of the 5′NTR, and that this RNA–RNA interaction modulates IRES-dependent translation in rabbit reticulocyte lysate and in HepG2 cells. The inclusion of the core-coding sequence (nucleotides 428–442) significantly suppressed the translational efficiency directed by HCV IRES in dicistronic reporter systems, and this suppression was relieved by site-directed mutations that blocked the long-range interaction between nucleotides 24–38 and 428–442. These findings suggest that the long-range interaction between the HCV 5′NTR and the core-coding nucleotide sequence down-regulate cap-independent translation via HCV IRES. The modulation of protein synthesis by long-range RNA–RNA interaction may play a role in the regulation of viral gene expression.</jats:p> |
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| spelling | KIM, YOON KI LEE, SONG HEE KIM, CHON SAENG SEOL, SU KYOUNG JANG, SUNG KEY 1355-8382 1469-9001 Cold Spring Harbor Laboratory Molecular Biology http://dx.doi.org/10.1261/rna.2185603 <jats:p>Hepatitis C virus (HCV) is a positive-sense RNA virus ∼9600 bases long. An internal ribosomal entry site (IRES) spans the 5′ nontranslated region, which is the most conserved and highly structured region of the HCV genome. In this study, we demonstrate that nucleotides 428–442 of the HCV core-coding sequence anneal to nucleotides 24–38 of the 5′NTR, and that this RNA–RNA interaction modulates IRES-dependent translation in rabbit reticulocyte lysate and in HepG2 cells. The inclusion of the core-coding sequence (nucleotides 428–442) significantly suppressed the translational efficiency directed by HCV IRES in dicistronic reporter systems, and this suppression was relieved by site-directed mutations that blocked the long-range interaction between nucleotides 24–38 and 428–442. These findings suggest that the long-range interaction between the HCV 5′NTR and the core-coding nucleotide sequence down-regulate cap-independent translation via HCV IRES. The modulation of protein synthesis by long-range RNA–RNA interaction may play a role in the regulation of viral gene expression.</jats:p> Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation RNA |
| spellingShingle | KIM, YOON KI, LEE, SONG HEE, KIM, CHON SAENG, SEOL, SU KYOUNG, JANG, SUNG KEY, RNA, Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation, Molecular Biology |
| title | Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_full | Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_fullStr | Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_full_unstemmed | Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_short | Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| title_sort | long-range rna–rna interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis c virus modulates the ires-dependent translation |
| title_unstemmed | Long-range RNA–RNA interaction between the 5′ nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation |
| topic | Molecular Biology |
| url | http://dx.doi.org/10.1261/rna.2185603 |