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Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions
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| Zeitschriftentitel: | Journal of Cell Science |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Cell Science, 2015 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Snyder, Joshua C. Rochelle, Lauren K. Marion, Sébastien Lyerly, H. Kim Barak, Larry S. Caron, Marc G. Snyder, Joshua C. Rochelle, Lauren K. Marion, Sébastien Lyerly, H. Kim Barak, Larry S. Caron, Marc G. |
|---|---|
| author |
Snyder, Joshua C. Rochelle, Lauren K. Marion, Sébastien Lyerly, H. Kim Barak, Larry S. Caron, Marc G. |
| spellingShingle |
Snyder, Joshua C. Rochelle, Lauren K. Marion, Sébastien Lyerly, H. Kim Barak, Larry S. Caron, Marc G. Journal of Cell Science Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions Cell Biology |
| author_sort |
snyder, joshua c. |
| spelling |
Snyder, Joshua C. Rochelle, Lauren K. Marion, Sébastien Lyerly, H. Kim Barak, Larry S. Caron, Marc G. 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.166322 <jats:p>Embryonic development and adult tissue homeostasis require precise information exchange between cells and their microenvironment to coordinate cell behavior. A specialized class of ultra-long actin-rich filopodia, termed cytonemes, provides one mechanism for this spatiotemporal regulation of extracellular cues. We provide here a mechanism whereby the stem cell marker Lgr5, and its family member Lgr4, promote the formation of cytonemes. Lgr4/5-induced cytonemes exceed lengths of 80 µm, are generated through stabilization of nascent filopodia from an underlying lamellipodial-like network, and functionally provide a pipeline for the transit of signaling effectors. As proof-of-principle, we demonstrate that Lgr5-induced cytonemes act as conduits for cell signaling by demonstrating that the actin-motor and filopodial cargo carrier protein Myosin X (Myo10) and the GCPR signaling effector ß-arrestin-2 (Arrb2) transit into cytonemes. This work delineates a biological function for Lgr4/5 and provides the rationale to fully investigate Lgr4/5 function and cytonemes in mammalian stem cell and cancer stem cell behavior.</jats:p> Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions Journal of Cell Science |
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| title |
Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_unstemmed |
Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_full |
Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_fullStr |
Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_full_unstemmed |
Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_short |
Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_sort |
lgr4 and lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1242/jcs.166322 |
| publishDate |
2015 |
| physical |
|
| description |
<jats:p>Embryonic development and adult tissue homeostasis require precise information exchange between cells and their microenvironment to coordinate cell behavior. A specialized class of ultra-long actin-rich filopodia, termed cytonemes, provides one mechanism for this spatiotemporal regulation of extracellular cues. We provide here a mechanism whereby the stem cell marker Lgr5, and its family member Lgr4, promote the formation of cytonemes. Lgr4/5-induced cytonemes exceed lengths of 80 µm, are generated through stabilization of nascent filopodia from an underlying lamellipodial-like network, and functionally provide a pipeline for the transit of signaling effectors. As proof-of-principle, we demonstrate that Lgr5-induced cytonemes act as conduits for cell signaling by demonstrating that the actin-motor and filopodial cargo carrier protein Myosin X (Myo10) and the GCPR signaling effector ß-arrestin-2 (Arrb2) transit into cytonemes. This work delineates a biological function for Lgr4/5 and provides the rationale to fully investigate Lgr4/5 function and cytonemes in mammalian stem cell and cancer stem cell behavior.</jats:p> |
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| author | Snyder, Joshua C., Rochelle, Lauren K., Marion, Sébastien, Lyerly, H. Kim, Barak, Larry S., Caron, Marc G. |
| author_facet | Snyder, Joshua C., Rochelle, Lauren K., Marion, Sébastien, Lyerly, H. Kim, Barak, Larry S., Caron, Marc G., Snyder, Joshua C., Rochelle, Lauren K., Marion, Sébastien, Lyerly, H. Kim, Barak, Larry S., Caron, Marc G. |
| author_sort | snyder, joshua c. |
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| description | <jats:p>Embryonic development and adult tissue homeostasis require precise information exchange between cells and their microenvironment to coordinate cell behavior. A specialized class of ultra-long actin-rich filopodia, termed cytonemes, provides one mechanism for this spatiotemporal regulation of extracellular cues. We provide here a mechanism whereby the stem cell marker Lgr5, and its family member Lgr4, promote the formation of cytonemes. Lgr4/5-induced cytonemes exceed lengths of 80 µm, are generated through stabilization of nascent filopodia from an underlying lamellipodial-like network, and functionally provide a pipeline for the transit of signaling effectors. As proof-of-principle, we demonstrate that Lgr5-induced cytonemes act as conduits for cell signaling by demonstrating that the actin-motor and filopodial cargo carrier protein Myosin X (Myo10) and the GCPR signaling effector ß-arrestin-2 (Arrb2) transit into cytonemes. This work delineates a biological function for Lgr4/5 and provides the rationale to fully investigate Lgr4/5 function and cytonemes in mammalian stem cell and cancer stem cell behavior.</jats:p> |
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| spelling | Snyder, Joshua C. Rochelle, Lauren K. Marion, Sébastien Lyerly, H. Kim Barak, Larry S. Caron, Marc G. 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.166322 <jats:p>Embryonic development and adult tissue homeostasis require precise information exchange between cells and their microenvironment to coordinate cell behavior. A specialized class of ultra-long actin-rich filopodia, termed cytonemes, provides one mechanism for this spatiotemporal regulation of extracellular cues. We provide here a mechanism whereby the stem cell marker Lgr5, and its family member Lgr4, promote the formation of cytonemes. Lgr4/5-induced cytonemes exceed lengths of 80 µm, are generated through stabilization of nascent filopodia from an underlying lamellipodial-like network, and functionally provide a pipeline for the transit of signaling effectors. As proof-of-principle, we demonstrate that Lgr5-induced cytonemes act as conduits for cell signaling by demonstrating that the actin-motor and filopodial cargo carrier protein Myosin X (Myo10) and the GCPR signaling effector ß-arrestin-2 (Arrb2) transit into cytonemes. This work delineates a biological function for Lgr4/5 and provides the rationale to fully investigate Lgr4/5 function and cytonemes in mammalian stem cell and cancer stem cell behavior.</jats:p> Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions Journal of Cell Science |
| spellingShingle | Snyder, Joshua C., Rochelle, Lauren K., Marion, Sébastien, Lyerly, H. Kim, Barak, Larry S., Caron, Marc G., Journal of Cell Science, Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions, Cell Biology |
| title | Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_full | Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_fullStr | Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_full_unstemmed | Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_short | Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_sort | lgr4 and lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| title_unstemmed | Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1242/jcs.166322 |