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Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes
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| Zeitschriftentitel: | Journal of Cell Science |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Cell Science, 117, 2004, 11, S. 2203-2214 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Doyle, Andrew Marganski, William Lee, Juliet Doyle, Andrew Marganski, William Lee, Juliet |
|---|---|
| author |
Doyle, Andrew Marganski, William Lee, Juliet |
| spellingShingle |
Doyle, Andrew Marganski, William Lee, Juliet Journal of Cell Science Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes Cell Biology |
| author_sort |
doyle, andrew |
| spelling |
Doyle, Andrew Marganski, William Lee, Juliet 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.01087 <jats:p>The coordination of protrusion with retraction is essential for continuous cell movement. In fish keratocytes the activation of stretch-activated calcium channels, and the resulting increase in intracellular calcium, trigger release of the rear cell margin when forward movement is impeded. Although it is likely that retraction involves a calcium-dependent increase in cytoskeletal contractility, it is not known how the timing, magnitude and localization of contractile forces are organized during retraction. We have addressed this question using a new gelatin traction force assay in combination with calcium imaging to determine what changes in cytoskeletal force production accompany calcium-induced retraction. We find that individual calcium transients are followed within seconds by a rapid increase in traction stress that is maintained, or increases in a stepwise manner, until retraction occurs. Increases in traction stress are accompanied by a distinct sequence of changes in the spatial distribution of large traction stresses. Regions of increased traction stress enlarge at the lateral cell margins and expand forward along the cell margin. In particular, rearward facing propulsive' tractions at the leading edge of the cell, which are normally very low, increase several fold. Following retraction, a precipitous drop in traction stress is observed. Such distinct variations in traction stress are not observed in cells when calcium transients are absent. These results suggest a mechanism by which global increases in intracellular calcium can locally regulate contractile force production, in order to maintain a rapid highly directed mode of movement.</jats:p> Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes Journal of Cell Science |
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| title |
Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_unstemmed |
Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_full |
Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_fullStr |
Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_full_unstemmed |
Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_short |
Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_sort |
calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1242/jcs.01087 |
| publishDate |
2004 |
| physical |
2203-2214 |
| description |
<jats:p>The coordination of protrusion with retraction is essential for continuous cell movement. In fish keratocytes the activation of stretch-activated calcium channels, and the resulting increase in intracellular calcium, trigger release of the rear cell margin when forward movement is impeded. Although it is likely that retraction involves a calcium-dependent increase in cytoskeletal contractility, it is not known how the timing, magnitude and localization of contractile forces are organized during retraction. We have addressed this question using a new gelatin traction force assay in combination with calcium imaging to determine what changes in cytoskeletal force production accompany calcium-induced retraction. We find that individual calcium transients are followed within seconds by a rapid increase in traction stress that is maintained, or increases in a stepwise manner, until retraction occurs. Increases in traction stress are accompanied by a distinct sequence of changes in the spatial distribution of large traction stresses. Regions of increased traction stress enlarge at the lateral cell margins and expand forward along the cell margin. In particular, rearward facing propulsive' tractions at the leading edge of the cell, which are normally very low, increase several fold. Following retraction, a precipitous drop in traction stress is observed. Such distinct variations in traction stress are not observed in cells when calcium transients are absent. These results suggest a mechanism by which global increases in intracellular calcium can locally regulate contractile force production, in order to maintain a rapid highly directed mode of movement.</jats:p> |
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| author | Doyle, Andrew, Marganski, William, Lee, Juliet |
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| description | <jats:p>The coordination of protrusion with retraction is essential for continuous cell movement. In fish keratocytes the activation of stretch-activated calcium channels, and the resulting increase in intracellular calcium, trigger release of the rear cell margin when forward movement is impeded. Although it is likely that retraction involves a calcium-dependent increase in cytoskeletal contractility, it is not known how the timing, magnitude and localization of contractile forces are organized during retraction. We have addressed this question using a new gelatin traction force assay in combination with calcium imaging to determine what changes in cytoskeletal force production accompany calcium-induced retraction. We find that individual calcium transients are followed within seconds by a rapid increase in traction stress that is maintained, or increases in a stepwise manner, until retraction occurs. Increases in traction stress are accompanied by a distinct sequence of changes in the spatial distribution of large traction stresses. Regions of increased traction stress enlarge at the lateral cell margins and expand forward along the cell margin. In particular, rearward facing propulsive' tractions at the leading edge of the cell, which are normally very low, increase several fold. Following retraction, a precipitous drop in traction stress is observed. Such distinct variations in traction stress are not observed in cells when calcium transients are absent. These results suggest a mechanism by which global increases in intracellular calcium can locally regulate contractile force production, in order to maintain a rapid highly directed mode of movement.</jats:p> |
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| spelling | Doyle, Andrew Marganski, William Lee, Juliet 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.01087 <jats:p>The coordination of protrusion with retraction is essential for continuous cell movement. In fish keratocytes the activation of stretch-activated calcium channels, and the resulting increase in intracellular calcium, trigger release of the rear cell margin when forward movement is impeded. Although it is likely that retraction involves a calcium-dependent increase in cytoskeletal contractility, it is not known how the timing, magnitude and localization of contractile forces are organized during retraction. We have addressed this question using a new gelatin traction force assay in combination with calcium imaging to determine what changes in cytoskeletal force production accompany calcium-induced retraction. We find that individual calcium transients are followed within seconds by a rapid increase in traction stress that is maintained, or increases in a stepwise manner, until retraction occurs. Increases in traction stress are accompanied by a distinct sequence of changes in the spatial distribution of large traction stresses. Regions of increased traction stress enlarge at the lateral cell margins and expand forward along the cell margin. In particular, rearward facing propulsive' tractions at the leading edge of the cell, which are normally very low, increase several fold. Following retraction, a precipitous drop in traction stress is observed. Such distinct variations in traction stress are not observed in cells when calcium transients are absent. These results suggest a mechanism by which global increases in intracellular calcium can locally regulate contractile force production, in order to maintain a rapid highly directed mode of movement.</jats:p> Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes Journal of Cell Science |
| spellingShingle | Doyle, Andrew, Marganski, William, Lee, Juliet, Journal of Cell Science, Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes, Cell Biology |
| title | Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_full | Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_fullStr | Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_full_unstemmed | Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_short | Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_sort | calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| title_unstemmed | Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1242/jcs.01087 |