Eintrag weiter verarbeiten
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye
Gespeichert in:
| Zeitschriftentitel: | Development |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Development, 132, 2005, 21, S. 4697-4707 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Rodrigues, Aloma B. Werner, Erica Moses, Kevin Rodrigues, Aloma B. Werner, Erica Moses, Kevin |
|---|---|
| author |
Rodrigues, Aloma B. Werner, Erica Moses, Kevin |
| spellingShingle |
Rodrigues, Aloma B. Werner, Erica Moses, Kevin Development Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye Developmental Biology Molecular Biology |
| author_sort |
rodrigues, aloma b. |
| spelling |
Rodrigues, Aloma B. Werner, Erica Moses, Kevin 1477-9129 0950-1991 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.02058 <jats:p>A key event in patterning the developing Drosophila compound eye is the progressive restriction of the transcription factor Atonal in the morphogenetic furrow. The Atonal pattern evolves from expression in all cells to an over-dispersed pattern of single founder cells (the future R8 photoreceptors). This restriction involves Notch-mediated lateral inhibition. However, there have been inconsistent data on a similar proposed role for the Egf receptor (Egfr). Experiments using a conditional Egfr mutation(Egfrtsla) suggested that Egfr does not regulate Atonal restriction, whereas experiments using Egfr-null mosaic Minute+ clones suggested that it does. Here, we have re-examined both approaches. We report that the lesion in Egfrtslais a serine to phenylalanine change in a conserved extracellular ligand-binding domain. We show by biochemical and genetic approaches that the Egfrtsla protein is rapidly and completely inactivated upon shift to the non-permissive temperature. We also find that on temperature shift the protein moves from the cell surface into the cell. Finally, we report a flaw in the Egfr-null mosaic Minute+ clone approach. Thus, we demonstrate that Egfr does not play a role in the initial specification or spacing of ommatidial founder cells.</jats:p> Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developing<i>Drosophila</i>eye Development |
| doi_str_mv |
10.1242/dev.02058 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9kZXYuMDIwNTg |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9kZXYuMDIwNTg |
| institution |
DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 |
| imprint |
The Company of Biologists, 2005 |
| imprint_str_mv |
The Company of Biologists, 2005 |
| issn |
1477-9129 0950-1991 |
| issn_str_mv |
1477-9129 0950-1991 |
| language |
English |
| mega_collection |
The Company of Biologists (CrossRef) |
| match_str |
rodrigues2005geneticandbiochemicalanalysisoftheroleofegfrinthemorphogeneticfurrowofthedevelopingdrosophilaeye |
| publishDateSort |
2005 |
| publisher |
The Company of Biologists |
| recordtype |
ai |
| record_format |
ai |
| series |
Development |
| source_id |
49 |
| title |
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_unstemmed |
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_full |
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_fullStr |
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_full_unstemmed |
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_short |
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_sort |
genetic and biochemical analysis of the role of egfr in the morphogenetic furrow of the developing<i>drosophila</i>eye |
| topic |
Developmental Biology Molecular Biology |
| url |
http://dx.doi.org/10.1242/dev.02058 |
| publishDate |
2005 |
| physical |
4697-4707 |
| description |
<jats:p>A key event in patterning the developing Drosophila compound eye is the progressive restriction of the transcription factor Atonal in the morphogenetic furrow. The Atonal pattern evolves from expression in all cells to an over-dispersed pattern of single founder cells (the future R8 photoreceptors). This restriction involves Notch-mediated lateral inhibition. However, there have been inconsistent data on a similar proposed role for the Egf receptor (Egfr). Experiments using a conditional Egfr mutation(Egfrtsla) suggested that Egfr does not regulate Atonal restriction, whereas experiments using Egfr-null mosaic Minute+ clones suggested that it does. Here, we have re-examined both approaches. We report that the lesion in Egfrtslais a serine to phenylalanine change in a conserved extracellular ligand-binding domain. We show by biochemical and genetic approaches that the Egfrtsla protein is rapidly and completely inactivated upon shift to the non-permissive temperature. We also find that on temperature shift the protein moves from the cell surface into the cell. Finally, we report a flaw in the Egfr-null mosaic Minute+ clone approach. Thus, we demonstrate that Egfr does not play a role in the initial specification or spacing of ommatidial founder cells.</jats:p> |
| container_issue |
21 |
| container_start_page |
4697 |
| container_title |
Development |
| container_volume |
132 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792330939672559623 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T13:33:02.589Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Genetic+and+biochemical+analysis+of+the+role+of+Egfr+in+the+morphogenetic+furrow+of+the+developingDrosophilaeye&rft.date=2005-11-01&genre=article&issn=0950-1991&volume=132&issue=21&spage=4697&epage=4707&pages=4697-4707&jtitle=Development&atitle=Genetic+and+biochemical+analysis+of+the+role+of+Egfr+in+the+morphogenetic+furrow+of+the+developing%3Ci%3EDrosophila%3C%2Fi%3Eeye&aulast=Moses&aufirst=Kevin&rft_id=info%3Adoi%2F10.1242%2Fdev.02058&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792330939672559623 |
| author | Rodrigues, Aloma B., Werner, Erica, Moses, Kevin |
| author_facet | Rodrigues, Aloma B., Werner, Erica, Moses, Kevin, Rodrigues, Aloma B., Werner, Erica, Moses, Kevin |
| author_sort | rodrigues, aloma b. |
| container_issue | 21 |
| container_start_page | 4697 |
| container_title | Development |
| container_volume | 132 |
| description | <jats:p>A key event in patterning the developing Drosophila compound eye is the progressive restriction of the transcription factor Atonal in the morphogenetic furrow. The Atonal pattern evolves from expression in all cells to an over-dispersed pattern of single founder cells (the future R8 photoreceptors). This restriction involves Notch-mediated lateral inhibition. However, there have been inconsistent data on a similar proposed role for the Egf receptor (Egfr). Experiments using a conditional Egfr mutation(Egfrtsla) suggested that Egfr does not regulate Atonal restriction, whereas experiments using Egfr-null mosaic Minute+ clones suggested that it does. Here, we have re-examined both approaches. We report that the lesion in Egfrtslais a serine to phenylalanine change in a conserved extracellular ligand-binding domain. We show by biochemical and genetic approaches that the Egfrtsla protein is rapidly and completely inactivated upon shift to the non-permissive temperature. We also find that on temperature shift the protein moves from the cell surface into the cell. Finally, we report a flaw in the Egfr-null mosaic Minute+ clone approach. Thus, we demonstrate that Egfr does not play a role in the initial specification or spacing of ommatidial founder cells.</jats:p> |
| doi_str_mv | 10.1242/dev.02058 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9kZXYuMDIwNTg |
| imprint | The Company of Biologists, 2005 |
| imprint_str_mv | The Company of Biologists, 2005 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
| issn | 1477-9129, 0950-1991 |
| issn_str_mv | 1477-9129, 0950-1991 |
| language | English |
| last_indexed | 2024-03-01T13:33:02.589Z |
| match_str | rodrigues2005geneticandbiochemicalanalysisoftheroleofegfrinthemorphogeneticfurrowofthedevelopingdrosophilaeye |
| mega_collection | The Company of Biologists (CrossRef) |
| physical | 4697-4707 |
| publishDate | 2005 |
| publishDateSort | 2005 |
| publisher | The Company of Biologists |
| record_format | ai |
| recordtype | ai |
| series | Development |
| source_id | 49 |
| spelling | Rodrigues, Aloma B. Werner, Erica Moses, Kevin 1477-9129 0950-1991 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.02058 <jats:p>A key event in patterning the developing Drosophila compound eye is the progressive restriction of the transcription factor Atonal in the morphogenetic furrow. The Atonal pattern evolves from expression in all cells to an over-dispersed pattern of single founder cells (the future R8 photoreceptors). This restriction involves Notch-mediated lateral inhibition. However, there have been inconsistent data on a similar proposed role for the Egf receptor (Egfr). Experiments using a conditional Egfr mutation(Egfrtsla) suggested that Egfr does not regulate Atonal restriction, whereas experiments using Egfr-null mosaic Minute+ clones suggested that it does. Here, we have re-examined both approaches. We report that the lesion in Egfrtslais a serine to phenylalanine change in a conserved extracellular ligand-binding domain. We show by biochemical and genetic approaches that the Egfrtsla protein is rapidly and completely inactivated upon shift to the non-permissive temperature. We also find that on temperature shift the protein moves from the cell surface into the cell. Finally, we report a flaw in the Egfr-null mosaic Minute+ clone approach. Thus, we demonstrate that Egfr does not play a role in the initial specification or spacing of ommatidial founder cells.</jats:p> Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developing<i>Drosophila</i>eye Development |
| spellingShingle | Rodrigues, Aloma B., Werner, Erica, Moses, Kevin, Development, Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye, Developmental Biology, Molecular Biology |
| title | Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_full | Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_fullStr | Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_full_unstemmed | Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_short | Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| title_sort | genetic and biochemical analysis of the role of egfr in the morphogenetic furrow of the developing<i>drosophila</i>eye |
| title_unstemmed | Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developingDrosophilaeye |
| topic | Developmental Biology, Molecular Biology |
| url | http://dx.doi.org/10.1242/dev.02058 |