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Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway

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Zeitschriftentitel: Biology Open
Personen und Körperschaften: Wang, Honggang, Zhong, Peiyu, Sun, Leilei
In: Biology Open, 2019
Format: E-Article
Sprache: Englisch
veröffentlicht:
The Company of Biologists
Schlagwörter:
General Agricultural and Biological Sciences
General Biochemistry, Genetics and Molecular Biology
author_facet Wang, Honggang
Zhong, Peiyu
Sun, Leilei
Wang, Honggang
Zhong, Peiyu
Sun, Leilei
author Wang, Honggang
Zhong, Peiyu
Sun, Leilei
spellingShingle Wang, Honggang
Zhong, Peiyu
Sun, Leilei
Biology Open
Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
General Agricultural and Biological Sciences
General Biochemistry, Genetics and Molecular Biology
author_sort wang, honggang
spelling Wang, Honggang Zhong, Peiyu Sun, Leilei 2046-6390 The Company of Biologists General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology http://dx.doi.org/10.1242/bio.043653 <jats:p>To investigate whether exogenous hydrogen sulfide (H2S) could mitigate NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway in L02 cells. L02 cells were stimulated with different concentrations of oleic acid (OA), then cell viability and the protein expression of NLRP3 and pro-caspase-1 were detected by MTT and western blot respectively to determine appropriate OA concentration used in this study. The cells were divided into 4 groups: the cells in control group were cultured with RPMI-1640 for 24.5 h; The cells in OA group were cultured with RPMI-1640 for 0.5 h, then were stimulated with 1.2 mmol/l OA for 24 h; The cells in NaHS+OA group were pretreated with sodium hydrogen sulfide (NaHS, a donor of H2S) 0.5 h before exposure to OA for 24 h. The cells in NaHS group were treated with NaHS 0.5 h, then were cultured with RPMI-1640 for 24 h. Subsequently the cells in every group were collected, the protein expression of NLRP3, procaspase-1, cleaved caspase-1, P62, LC3, Beclin1, T-AMPK, P-AMPK, T-mTOR, P-mTOR and the level of IL-1β were detected by western blot and ElISA respectively. Exogenous H2S reduced the level of NLRP3, caspase-1, P62, IL-1β and the ratio of P-mTOR/T-mTOR induced by OA, increased the ratio of LC3 II/I and the protein expression of Beclin1 suppressed by OA. This study demonstrated for the first time that H2S might suppress NLRP3 inflammasome-mediated inflammation induced by OA through promoting autophagy via AMPK-mTOR pathway. It provides a theoretical basis for the further study of the anti-inflammatory mechanism of H2S.</jats:p> Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway Biology Open
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title Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_unstemmed Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_full Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_fullStr Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_full_unstemmed Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_short Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_sort exogenous hydrogen sulfide mitigates nlrp3 inflammasome-mediated inflammation through promoting autophagy via ampk-mtor pathway
topic General Agricultural and Biological Sciences
General Biochemistry, Genetics and Molecular Biology
url http://dx.doi.org/10.1242/bio.043653
publishDate 2019
physical
description <jats:p>To investigate whether exogenous hydrogen sulfide (H2S) could mitigate NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway in L02 cells. L02 cells were stimulated with different concentrations of oleic acid (OA), then cell viability and the protein expression of NLRP3 and pro-caspase-1 were detected by MTT and western blot respectively to determine appropriate OA concentration used in this study. The cells were divided into 4 groups: the cells in control group were cultured with RPMI-1640 for 24.5 h; The cells in OA group were cultured with RPMI-1640 for 0.5 h, then were stimulated with 1.2 mmol/l OA for 24 h; The cells in NaHS+OA group were pretreated with sodium hydrogen sulfide (NaHS, a donor of H2S) 0.5 h before exposure to OA for 24 h. The cells in NaHS group were treated with NaHS 0.5 h, then were cultured with RPMI-1640 for 24 h. Subsequently the cells in every group were collected, the protein expression of NLRP3, procaspase-1, cleaved caspase-1, P62, LC3, Beclin1, T-AMPK, P-AMPK, T-mTOR, P-mTOR and the level of IL-1β were detected by western blot and ElISA respectively. Exogenous H2S reduced the level of NLRP3, caspase-1, P62, IL-1β and the ratio of P-mTOR/T-mTOR induced by OA, increased the ratio of LC3 II/I and the protein expression of Beclin1 suppressed by OA. This study demonstrated for the first time that H2S might suppress NLRP3 inflammasome-mediated inflammation induced by OA through promoting autophagy via AMPK-mTOR pathway. It provides a theoretical basis for the further study of the anti-inflammatory mechanism of H2S.</jats:p>
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author Wang, Honggang, Zhong, Peiyu, Sun, Leilei
author_facet Wang, Honggang, Zhong, Peiyu, Sun, Leilei, Wang, Honggang, Zhong, Peiyu, Sun, Leilei
author_sort wang, honggang
container_start_page 0
container_title Biology Open
description <jats:p>To investigate whether exogenous hydrogen sulfide (H2S) could mitigate NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway in L02 cells. L02 cells were stimulated with different concentrations of oleic acid (OA), then cell viability and the protein expression of NLRP3 and pro-caspase-1 were detected by MTT and western blot respectively to determine appropriate OA concentration used in this study. The cells were divided into 4 groups: the cells in control group were cultured with RPMI-1640 for 24.5 h; The cells in OA group were cultured with RPMI-1640 for 0.5 h, then were stimulated with 1.2 mmol/l OA for 24 h; The cells in NaHS+OA group were pretreated with sodium hydrogen sulfide (NaHS, a donor of H2S) 0.5 h before exposure to OA for 24 h. The cells in NaHS group were treated with NaHS 0.5 h, then were cultured with RPMI-1640 for 24 h. Subsequently the cells in every group were collected, the protein expression of NLRP3, procaspase-1, cleaved caspase-1, P62, LC3, Beclin1, T-AMPK, P-AMPK, T-mTOR, P-mTOR and the level of IL-1β were detected by western blot and ElISA respectively. Exogenous H2S reduced the level of NLRP3, caspase-1, P62, IL-1β and the ratio of P-mTOR/T-mTOR induced by OA, increased the ratio of LC3 II/I and the protein expression of Beclin1 suppressed by OA. This study demonstrated for the first time that H2S might suppress NLRP3 inflammasome-mediated inflammation induced by OA through promoting autophagy via AMPK-mTOR pathway. It provides a theoretical basis for the further study of the anti-inflammatory mechanism of H2S.</jats:p>
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spelling Wang, Honggang Zhong, Peiyu Sun, Leilei 2046-6390 The Company of Biologists General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology http://dx.doi.org/10.1242/bio.043653 <jats:p>To investigate whether exogenous hydrogen sulfide (H2S) could mitigate NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway in L02 cells. L02 cells were stimulated with different concentrations of oleic acid (OA), then cell viability and the protein expression of NLRP3 and pro-caspase-1 were detected by MTT and western blot respectively to determine appropriate OA concentration used in this study. The cells were divided into 4 groups: the cells in control group were cultured with RPMI-1640 for 24.5 h; The cells in OA group were cultured with RPMI-1640 for 0.5 h, then were stimulated with 1.2 mmol/l OA for 24 h; The cells in NaHS+OA group were pretreated with sodium hydrogen sulfide (NaHS, a donor of H2S) 0.5 h before exposure to OA for 24 h. The cells in NaHS group were treated with NaHS 0.5 h, then were cultured with RPMI-1640 for 24 h. Subsequently the cells in every group were collected, the protein expression of NLRP3, procaspase-1, cleaved caspase-1, P62, LC3, Beclin1, T-AMPK, P-AMPK, T-mTOR, P-mTOR and the level of IL-1β were detected by western blot and ElISA respectively. Exogenous H2S reduced the level of NLRP3, caspase-1, P62, IL-1β and the ratio of P-mTOR/T-mTOR induced by OA, increased the ratio of LC3 II/I and the protein expression of Beclin1 suppressed by OA. This study demonstrated for the first time that H2S might suppress NLRP3 inflammasome-mediated inflammation induced by OA through promoting autophagy via AMPK-mTOR pathway. It provides a theoretical basis for the further study of the anti-inflammatory mechanism of H2S.</jats:p> Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway Biology Open
spellingShingle Wang, Honggang, Zhong, Peiyu, Sun, Leilei, Biology Open, Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology
title Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_full Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_fullStr Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_full_unstemmed Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_short Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
title_sort exogenous hydrogen sulfide mitigates nlrp3 inflammasome-mediated inflammation through promoting autophagy via ampk-mtor pathway
title_unstemmed Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
topic General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology
url http://dx.doi.org/10.1242/bio.043653