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Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence
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| Zeitschriftentitel: | Canadian Journal of Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Canadian Journal of Microbiology, 64, 2018, 1, S. 57-68 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Canadian Science Publishing
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| Schlagwörter: |
| author_facet |
Lin, Huijiao Jiang, Xiang Yi, Jianping Wang, Xinguo Zuo, Ranling Jiang, Zide Wang, Weifang Zhou, Erxun Lin, Huijiao Jiang, Xiang Yi, Jianping Wang, Xinguo Zuo, Ranling Jiang, Zide Wang, Weifang Zhou, Erxun |
|---|---|
| author |
Lin, Huijiao Jiang, Xiang Yi, Jianping Wang, Xinguo Zuo, Ranling Jiang, Zide Wang, Weifang Zhou, Erxun |
| spellingShingle |
Lin, Huijiao Jiang, Xiang Yi, Jianping Wang, Xinguo Zuo, Ranling Jiang, Zide Wang, Weifang Zhou, Erxun Canadian Journal of Microbiology Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
| author_sort |
lin, huijiao |
| spelling |
Lin, Huijiao Jiang, Xiang Yi, Jianping Wang, Xinguo Zuo, Ranling Jiang, Zide Wang, Weifang Zhou, Erxun 0008-4166 1480-3275 Canadian Science Publishing Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology http://dx.doi.org/10.1139/cjm-2017-0448 <jats:p>A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull’s-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull’s-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull’s-eye rot pathogens in port quarantine and orchard epidemiological studies.</jats:p> Molecular identification of<i>Neofabraea</i>species associated with bull’s-eye rot on apple using rolling-circle amplification of partial<i>EF-1α</i>sequence Canadian Journal of Microbiology |
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10.1139/cjm-2017-0448 |
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Canadian Science Publishing, 2018 |
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| title |
Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_unstemmed |
Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_full |
Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_fullStr |
Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_full_unstemmed |
Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_short |
Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_sort |
molecular identification of<i>neofabraea</i>species associated with bull’s-eye rot on apple using rolling-circle amplification of partial<i>ef-1α</i>sequence |
| topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
| url |
http://dx.doi.org/10.1139/cjm-2017-0448 |
| publishDate |
2018 |
| physical |
57-68 |
| description |
<jats:p>A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull’s-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull’s-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull’s-eye rot pathogens in port quarantine and orchard epidemiological studies.</jats:p> |
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| author | Lin, Huijiao, Jiang, Xiang, Yi, Jianping, Wang, Xinguo, Zuo, Ranling, Jiang, Zide, Wang, Weifang, Zhou, Erxun |
| author_facet | Lin, Huijiao, Jiang, Xiang, Yi, Jianping, Wang, Xinguo, Zuo, Ranling, Jiang, Zide, Wang, Weifang, Zhou, Erxun, Lin, Huijiao, Jiang, Xiang, Yi, Jianping, Wang, Xinguo, Zuo, Ranling, Jiang, Zide, Wang, Weifang, Zhou, Erxun |
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| description | <jats:p>A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull’s-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull’s-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull’s-eye rot pathogens in port quarantine and orchard epidemiological studies.</jats:p> |
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| spelling | Lin, Huijiao Jiang, Xiang Yi, Jianping Wang, Xinguo Zuo, Ranling Jiang, Zide Wang, Weifang Zhou, Erxun 0008-4166 1480-3275 Canadian Science Publishing Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology http://dx.doi.org/10.1139/cjm-2017-0448 <jats:p>A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull’s-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull’s-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull’s-eye rot pathogens in port quarantine and orchard epidemiological studies.</jats:p> Molecular identification of<i>Neofabraea</i>species associated with bull’s-eye rot on apple using rolling-circle amplification of partial<i>EF-1α</i>sequence Canadian Journal of Microbiology |
| spellingShingle | Lin, Huijiao, Jiang, Xiang, Yi, Jianping, Wang, Xinguo, Zuo, Ranling, Jiang, Zide, Wang, Weifang, Zhou, Erxun, Canadian Journal of Microbiology, Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence, Genetics, Molecular Biology, Applied Microbiology and Biotechnology, General Medicine, Immunology, Microbiology |
| title | Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_full | Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_fullStr | Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_full_unstemmed | Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_short | Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| title_sort | molecular identification of<i>neofabraea</i>species associated with bull’s-eye rot on apple using rolling-circle amplification of partial<i>ef-1α</i>sequence |
| title_unstemmed | Molecular identification ofNeofabraeaspecies associated with bull’s-eye rot on apple using rolling-circle amplification of partialEF-1αsequence |
| topic | Genetics, Molecular Biology, Applied Microbiology and Biotechnology, General Medicine, Immunology, Microbiology |
| url | http://dx.doi.org/10.1139/cjm-2017-0448 |