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Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells
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| Zeitschriftentitel: | Biochemistry and Cell Biology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Biochemistry and Cell Biology, 93, 2015, 4, S. 376-384 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Canadian Science Publishing
|
| Schlagwörter: |
| author_facet |
González-Duarte, Ramiro José Cázares-Ordoñez, Verna Romero-Córdoba, Sandra Díaz, Lorenza Ortíz, Víctor Freyre-González, Julio Augusto Hidalgo-Miranda, Alfredo Larrea, Fernando Avila, Euclides González-Duarte, Ramiro José Cázares-Ordoñez, Verna Romero-Córdoba, Sandra Díaz, Lorenza Ortíz, Víctor Freyre-González, Julio Augusto Hidalgo-Miranda, Alfredo Larrea, Fernando Avila, Euclides |
|---|---|
| author |
González-Duarte, Ramiro José Cázares-Ordoñez, Verna Romero-Córdoba, Sandra Díaz, Lorenza Ortíz, Víctor Freyre-González, Julio Augusto Hidalgo-Miranda, Alfredo Larrea, Fernando Avila, Euclides |
| spellingShingle |
González-Duarte, Ramiro José Cázares-Ordoñez, Verna Romero-Córdoba, Sandra Díaz, Lorenza Ortíz, Víctor Freyre-González, Julio Augusto Hidalgo-Miranda, Alfredo Larrea, Fernando Avila, Euclides Biochemistry and Cell Biology Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells Cell Biology Molecular Biology Biochemistry |
| author_sort |
gonzález-duarte, ramiro josé |
| spelling |
González-Duarte, Ramiro José Cázares-Ordoñez, Verna Romero-Córdoba, Sandra Díaz, Lorenza Ortíz, Víctor Freyre-González, Julio Augusto Hidalgo-Miranda, Alfredo Larrea, Fernando Avila, Euclides 0829-8211 1208-6002 Canadian Science Publishing Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1139/bcb-2015-0010 <jats:p> MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D<jats:sub>3</jats:sub>, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol. </jats:p> Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells Biochemistry and Cell Biology |
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| title |
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_unstemmed |
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_full |
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_fullStr |
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_full_unstemmed |
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_short |
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_sort |
calcitriol increases dicer expression and modifies the micrornas signature in siha cervical cancer cells |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1139/bcb-2015-0010 |
| publishDate |
2015 |
| physical |
376-384 |
| description |
<jats:p> MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D<jats:sub>3</jats:sub>, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol. </jats:p> |
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| author_facet | González-Duarte, Ramiro José, Cázares-Ordoñez, Verna, Romero-Córdoba, Sandra, Díaz, Lorenza, Ortíz, Víctor, Freyre-González, Julio Augusto, Hidalgo-Miranda, Alfredo, Larrea, Fernando, Avila, Euclides, González-Duarte, Ramiro José, Cázares-Ordoñez, Verna, Romero-Córdoba, Sandra, Díaz, Lorenza, Ortíz, Víctor, Freyre-González, Julio Augusto, Hidalgo-Miranda, Alfredo, Larrea, Fernando, Avila, Euclides |
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| description | <jats:p> MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D<jats:sub>3</jats:sub>, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol. </jats:p> |
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| spelling | González-Duarte, Ramiro José Cázares-Ordoñez, Verna Romero-Córdoba, Sandra Díaz, Lorenza Ortíz, Víctor Freyre-González, Julio Augusto Hidalgo-Miranda, Alfredo Larrea, Fernando Avila, Euclides 0829-8211 1208-6002 Canadian Science Publishing Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1139/bcb-2015-0010 <jats:p> MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D<jats:sub>3</jats:sub>, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol. </jats:p> Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells Biochemistry and Cell Biology |
| spellingShingle | González-Duarte, Ramiro José, Cázares-Ordoñez, Verna, Romero-Córdoba, Sandra, Díaz, Lorenza, Ortíz, Víctor, Freyre-González, Julio Augusto, Hidalgo-Miranda, Alfredo, Larrea, Fernando, Avila, Euclides, Biochemistry and Cell Biology, Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells, Cell Biology, Molecular Biology, Biochemistry |
| title | Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_full | Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_fullStr | Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_full_unstemmed | Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_short | Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| title_sort | calcitriol increases dicer expression and modifies the micrornas signature in siha cervical cancer cells |
| title_unstemmed | Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1139/bcb-2015-0010 |