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Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting

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Bibliographische Detailangaben
Zeitschriftentitel: Canadian Journal of Microbiology
Personen und Körperschaften: Cimolai, Nevio, Thomas, Eva E, Tan, Rusung, Hill, Alan
In: Canadian Journal of Microbiology, 47, 2001, 5, S. 392-396
Format: E-Article
Sprache: Englisch
veröffentlicht:
Canadian Science Publishing
Schlagwörter:
Genetics
Molecular Biology
Applied Microbiology and Biotechnology
General Medicine
Immunology
Microbiology
author_facet Cimolai, Nevio
Thomas, Eva E
Tan, Rusung
Hill, Alan
Cimolai, Nevio
Thomas, Eva E
Tan, Rusung
Hill, Alan
author Cimolai, Nevio
Thomas, Eva E
Tan, Rusung
Hill, Alan
spellingShingle Cimolai, Nevio
Thomas, Eva E
Tan, Rusung
Hill, Alan
Canadian Journal of Microbiology
Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
Genetics
Molecular Biology
Applied Microbiology and Biotechnology
General Medicine
Immunology
Microbiology
author_sort cimolai, nevio
spelling Cimolai, Nevio Thomas, Eva E Tan, Rusung Hill, Alan 0008-4166 1480-3275 Canadian Science Publishing Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology http://dx.doi.org/10.1139/w01-028 <jats:p>An assessment was made of the utilization and impact of a diagnostic polymerase chain reaction (PCR) assay for the diagnosis of herpes simplex viruses (HSV) 1 and 2 in cerebrospinal fluid of children who attended a Canadian pediatric referral centre. One hundred and three assays were performed on specimens from 103 patients during the period August 1997 to September 1998. Patient ages ranged from newborn to 16 years. Indications for HSV PCR included seizures with or without fever (56.3%), aseptic meningitis (16.5%), and encephalopathy with or without fever (10.7%). Only 2 of 103 (1.9%) assays were positive, including one each for HSV1 and HSV2. Control specimens that were seeded with virus indicated inhibition for 24.3, 8.8, and 6.8% of assays for HSV1, HSV2, and both HSV1 and HSV2, respectively. The mean turn-around time for HSV PCR was 2.5 days, and 90.3% were completed in less than 5 days. Acyclovir was administered to 78.6% of the patients overall; the results of the HSV PCR impacted on the treatment courses for 36 individuals. Nevertheless, 16.5% of patients continued to receive extended courses of antiviral therapy despite negative HSV PCR assays. Although it is desirable to decrease the frequency of PCR inhibitions and to further decrease the interval to assay completion, HSV PCR does have a significant impact on antiviral use in this setting.Key words: herpes simplex, polymerase chain reaction, central nervous system.</jats:p> Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting Canadian Journal of Microbiology
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title Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_unstemmed Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_full Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_fullStr Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_full_unstemmed Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_short Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_sort utilization of herpes simplex pcr assays for cerebrospinal fluid in a pediatric health care setting
topic Genetics
Molecular Biology
Applied Microbiology and Biotechnology
General Medicine
Immunology
Microbiology
url http://dx.doi.org/10.1139/w01-028
publishDate 2001
physical 392-396
description <jats:p>An assessment was made of the utilization and impact of a diagnostic polymerase chain reaction (PCR) assay for the diagnosis of herpes simplex viruses (HSV) 1 and 2 in cerebrospinal fluid of children who attended a Canadian pediatric referral centre. One hundred and three assays were performed on specimens from 103 patients during the period August 1997 to September 1998. Patient ages ranged from newborn to 16 years. Indications for HSV PCR included seizures with or without fever (56.3%), aseptic meningitis (16.5%), and encephalopathy with or without fever (10.7%). Only 2 of 103 (1.9%) assays were positive, including one each for HSV1 and HSV2. Control specimens that were seeded with virus indicated inhibition for 24.3, 8.8, and 6.8% of assays for HSV1, HSV2, and both HSV1 and HSV2, respectively. The mean turn-around time for HSV PCR was 2.5 days, and 90.3% were completed in less than 5 days. Acyclovir was administered to 78.6% of the patients overall; the results of the HSV PCR impacted on the treatment courses for 36 individuals. Nevertheless, 16.5% of patients continued to receive extended courses of antiviral therapy despite negative HSV PCR assays. Although it is desirable to decrease the frequency of PCR inhibitions and to further decrease the interval to assay completion, HSV PCR does have a significant impact on antiviral use in this setting.Key words: herpes simplex, polymerase chain reaction, central nervous system.</jats:p>
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author Cimolai, Nevio, Thomas, Eva E, Tan, Rusung, Hill, Alan
author_facet Cimolai, Nevio, Thomas, Eva E, Tan, Rusung, Hill, Alan, Cimolai, Nevio, Thomas, Eva E, Tan, Rusung, Hill, Alan
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container_issue 5
container_start_page 392
container_title Canadian Journal of Microbiology
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description <jats:p>An assessment was made of the utilization and impact of a diagnostic polymerase chain reaction (PCR) assay for the diagnosis of herpes simplex viruses (HSV) 1 and 2 in cerebrospinal fluid of children who attended a Canadian pediatric referral centre. One hundred and three assays were performed on specimens from 103 patients during the period August 1997 to September 1998. Patient ages ranged from newborn to 16 years. Indications for HSV PCR included seizures with or without fever (56.3%), aseptic meningitis (16.5%), and encephalopathy with or without fever (10.7%). Only 2 of 103 (1.9%) assays were positive, including one each for HSV1 and HSV2. Control specimens that were seeded with virus indicated inhibition for 24.3, 8.8, and 6.8% of assays for HSV1, HSV2, and both HSV1 and HSV2, respectively. The mean turn-around time for HSV PCR was 2.5 days, and 90.3% were completed in less than 5 days. Acyclovir was administered to 78.6% of the patients overall; the results of the HSV PCR impacted on the treatment courses for 36 individuals. Nevertheless, 16.5% of patients continued to receive extended courses of antiviral therapy despite negative HSV PCR assays. Although it is desirable to decrease the frequency of PCR inhibitions and to further decrease the interval to assay completion, HSV PCR does have a significant impact on antiviral use in this setting.Key words: herpes simplex, polymerase chain reaction, central nervous system.</jats:p>
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spelling Cimolai, Nevio Thomas, Eva E Tan, Rusung Hill, Alan 0008-4166 1480-3275 Canadian Science Publishing Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology http://dx.doi.org/10.1139/w01-028 <jats:p>An assessment was made of the utilization and impact of a diagnostic polymerase chain reaction (PCR) assay for the diagnosis of herpes simplex viruses (HSV) 1 and 2 in cerebrospinal fluid of children who attended a Canadian pediatric referral centre. One hundred and three assays were performed on specimens from 103 patients during the period August 1997 to September 1998. Patient ages ranged from newborn to 16 years. Indications for HSV PCR included seizures with or without fever (56.3%), aseptic meningitis (16.5%), and encephalopathy with or without fever (10.7%). Only 2 of 103 (1.9%) assays were positive, including one each for HSV1 and HSV2. Control specimens that were seeded with virus indicated inhibition for 24.3, 8.8, and 6.8% of assays for HSV1, HSV2, and both HSV1 and HSV2, respectively. The mean turn-around time for HSV PCR was 2.5 days, and 90.3% were completed in less than 5 days. Acyclovir was administered to 78.6% of the patients overall; the results of the HSV PCR impacted on the treatment courses for 36 individuals. Nevertheless, 16.5% of patients continued to receive extended courses of antiviral therapy despite negative HSV PCR assays. Although it is desirable to decrease the frequency of PCR inhibitions and to further decrease the interval to assay completion, HSV PCR does have a significant impact on antiviral use in this setting.Key words: herpes simplex, polymerase chain reaction, central nervous system.</jats:p> Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting Canadian Journal of Microbiology
spellingShingle Cimolai, Nevio, Thomas, Eva E, Tan, Rusung, Hill, Alan, Canadian Journal of Microbiology, Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting, Genetics, Molecular Biology, Applied Microbiology and Biotechnology, General Medicine, Immunology, Microbiology
title Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_full Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_fullStr Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_full_unstemmed Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_short Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
title_sort utilization of herpes simplex pcr assays for cerebrospinal fluid in a pediatric health care setting
title_unstemmed Utilization of herpes simplex PCR assays for cerebrospinal fluid in a pediatric health care setting
topic Genetics, Molecular Biology, Applied Microbiology and Biotechnology, General Medicine, Immunology, Microbiology
url http://dx.doi.org/10.1139/w01-028