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Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078
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| Zeitschriftentitel: | mSystems |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , |
| In: | mSystems, 5, 2020, 3 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Li, Tinghua Zhang, Yan Dong, Ke Kuo, Chih-Jung Li, Chong Zhu, Yong-Qiang Qin, Jinhong Li, Qing-Tian Chang, Yung-Fu Guo, Xiaokui Zhu, Yongzhang Li, Tinghua Zhang, Yan Dong, Ke Kuo, Chih-Jung Li, Chong Zhu, Yong-Qiang Qin, Jinhong Li, Qing-Tian Chang, Yung-Fu Guo, Xiaokui Zhu, Yongzhang |
|---|---|
| author |
Li, Tinghua Zhang, Yan Dong, Ke Kuo, Chih-Jung Li, Chong Zhu, Yong-Qiang Qin, Jinhong Li, Qing-Tian Chang, Yung-Fu Guo, Xiaokui Zhu, Yongzhang |
| spellingShingle |
Li, Tinghua Zhang, Yan Dong, Ke Kuo, Chih-Jung Li, Chong Zhu, Yong-Qiang Qin, Jinhong Li, Qing-Tian Chang, Yung-Fu Guo, Xiaokui Zhu, Yongzhang mSystems Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 Computer Science Applications Genetics Molecular Biology Modeling and Simulation Ecology, Evolution, Behavior and Systematics Biochemistry Physiology Microbiology |
| author_sort |
li, tinghua |
| spelling |
Li, Tinghua Zhang, Yan Dong, Ke Kuo, Chih-Jung Li, Chong Zhu, Yong-Qiang Qin, Jinhong Li, Qing-Tian Chang, Yung-Fu Guo, Xiaokui Zhu, Yongzhang 2379-5077 American Society for Microbiology Computer Science Applications Genetics Molecular Biology Modeling and Simulation Ecology, Evolution, Behavior and Systematics Biochemistry Physiology Microbiology http://dx.doi.org/10.1128/msystems.00017-20 <jats:p> <jats:named-content content-type="genus-species">C. difficile</jats:named-content> is one of the most clinically significant intestinal pathogens. Although phages have been shown to effectively control <jats:named-content content-type="genus-species">C. difficile</jats:named-content> infection, the host responses to phage predation have not been fully studied. In this study, we reported the isolation and characterization of a new phage, JD032, and analyzed the global transcriptomic changes in the hypervirulent RT078 <jats:named-content content-type="genus-species">C. difficile</jats:named-content> strain, TW11, during phage JD032 infection. We found that bacterial host mRNA was progressively replaced with phage transcripts, three temporal categories of JD032 gene expression, the extensive interplay between phage-bacterium, antiphage-like responses of the host and phage evasion, and decreased expression of sporulation- and virulence-related genes of the host after phage infection. These findings confirmed the complexity of interactions between <jats:named-content content-type="genus-species">C. difficile</jats:named-content> and phages and suggest that phages undergoing a lytic cycle may also cause different phenotypes in hosts, similar to prophages, which may inspire phage therapy for the control of <jats:named-content content-type="genus-species">C. difficile</jats:named-content> . </jats:p> Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 mSystems |
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| title |
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_unstemmed |
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_full |
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_fullStr |
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_full_unstemmed |
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_short |
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_sort |
isolation and characterization of the novel phage jd032 and global transcriptomic response during jd032 infection of clostridioides difficile ribotype 078 |
| topic |
Computer Science Applications Genetics Molecular Biology Modeling and Simulation Ecology, Evolution, Behavior and Systematics Biochemistry Physiology Microbiology |
| url |
http://dx.doi.org/10.1128/msystems.00017-20 |
| publishDate |
2020 |
| physical |
|
| description |
<jats:p>
<jats:named-content content-type="genus-species">C. difficile</jats:named-content>
is one of the most clinically significant intestinal pathogens. Although phages have been shown to effectively control
<jats:named-content content-type="genus-species">C. difficile</jats:named-content>
infection, the host responses to phage predation have not been fully studied. In this study, we reported the isolation and characterization of a new phage, JD032, and analyzed the global transcriptomic changes in the hypervirulent RT078
<jats:named-content content-type="genus-species">C. difficile</jats:named-content>
strain, TW11, during phage JD032 infection. We found that bacterial host mRNA was progressively replaced with phage transcripts, three temporal categories of JD032 gene expression, the extensive interplay between phage-bacterium, antiphage-like responses of the host and phage evasion, and decreased expression of sporulation- and virulence-related genes of the host after phage infection. These findings confirmed the complexity of interactions between
<jats:named-content content-type="genus-species">C. difficile</jats:named-content>
and phages and suggest that phages undergoing a lytic cycle may also cause different phenotypes in hosts, similar to prophages, which may inspire phage therapy for the control of
<jats:named-content content-type="genus-species">C. difficile</jats:named-content>
.
</jats:p> |
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| author | Li, Tinghua, Zhang, Yan, Dong, Ke, Kuo, Chih-Jung, Li, Chong, Zhu, Yong-Qiang, Qin, Jinhong, Li, Qing-Tian, Chang, Yung-Fu, Guo, Xiaokui, Zhu, Yongzhang |
| author_facet | Li, Tinghua, Zhang, Yan, Dong, Ke, Kuo, Chih-Jung, Li, Chong, Zhu, Yong-Qiang, Qin, Jinhong, Li, Qing-Tian, Chang, Yung-Fu, Guo, Xiaokui, Zhu, Yongzhang, Li, Tinghua, Zhang, Yan, Dong, Ke, Kuo, Chih-Jung, Li, Chong, Zhu, Yong-Qiang, Qin, Jinhong, Li, Qing-Tian, Chang, Yung-Fu, Guo, Xiaokui, Zhu, Yongzhang |
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| description | <jats:p> <jats:named-content content-type="genus-species">C. difficile</jats:named-content> is one of the most clinically significant intestinal pathogens. Although phages have been shown to effectively control <jats:named-content content-type="genus-species">C. difficile</jats:named-content> infection, the host responses to phage predation have not been fully studied. In this study, we reported the isolation and characterization of a new phage, JD032, and analyzed the global transcriptomic changes in the hypervirulent RT078 <jats:named-content content-type="genus-species">C. difficile</jats:named-content> strain, TW11, during phage JD032 infection. We found that bacterial host mRNA was progressively replaced with phage transcripts, three temporal categories of JD032 gene expression, the extensive interplay between phage-bacterium, antiphage-like responses of the host and phage evasion, and decreased expression of sporulation- and virulence-related genes of the host after phage infection. These findings confirmed the complexity of interactions between <jats:named-content content-type="genus-species">C. difficile</jats:named-content> and phages and suggest that phages undergoing a lytic cycle may also cause different phenotypes in hosts, similar to prophages, which may inspire phage therapy for the control of <jats:named-content content-type="genus-species">C. difficile</jats:named-content> . </jats:p> |
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| spelling | Li, Tinghua Zhang, Yan Dong, Ke Kuo, Chih-Jung Li, Chong Zhu, Yong-Qiang Qin, Jinhong Li, Qing-Tian Chang, Yung-Fu Guo, Xiaokui Zhu, Yongzhang 2379-5077 American Society for Microbiology Computer Science Applications Genetics Molecular Biology Modeling and Simulation Ecology, Evolution, Behavior and Systematics Biochemistry Physiology Microbiology http://dx.doi.org/10.1128/msystems.00017-20 <jats:p> <jats:named-content content-type="genus-species">C. difficile</jats:named-content> is one of the most clinically significant intestinal pathogens. Although phages have been shown to effectively control <jats:named-content content-type="genus-species">C. difficile</jats:named-content> infection, the host responses to phage predation have not been fully studied. In this study, we reported the isolation and characterization of a new phage, JD032, and analyzed the global transcriptomic changes in the hypervirulent RT078 <jats:named-content content-type="genus-species">C. difficile</jats:named-content> strain, TW11, during phage JD032 infection. We found that bacterial host mRNA was progressively replaced with phage transcripts, three temporal categories of JD032 gene expression, the extensive interplay between phage-bacterium, antiphage-like responses of the host and phage evasion, and decreased expression of sporulation- and virulence-related genes of the host after phage infection. These findings confirmed the complexity of interactions between <jats:named-content content-type="genus-species">C. difficile</jats:named-content> and phages and suggest that phages undergoing a lytic cycle may also cause different phenotypes in hosts, similar to prophages, which may inspire phage therapy for the control of <jats:named-content content-type="genus-species">C. difficile</jats:named-content> . </jats:p> Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 mSystems |
| spellingShingle | Li, Tinghua, Zhang, Yan, Dong, Ke, Kuo, Chih-Jung, Li, Chong, Zhu, Yong-Qiang, Qin, Jinhong, Li, Qing-Tian, Chang, Yung-Fu, Guo, Xiaokui, Zhu, Yongzhang, mSystems, Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078, Computer Science Applications, Genetics, Molecular Biology, Modeling and Simulation, Ecology, Evolution, Behavior and Systematics, Biochemistry, Physiology, Microbiology |
| title | Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_full | Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_fullStr | Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_full_unstemmed | Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_short | Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| title_sort | isolation and characterization of the novel phage jd032 and global transcriptomic response during jd032 infection of clostridioides difficile ribotype 078 |
| title_unstemmed | Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078 |
| topic | Computer Science Applications, Genetics, Molecular Biology, Modeling and Simulation, Ecology, Evolution, Behavior and Systematics, Biochemistry, Physiology, Microbiology |
| url | http://dx.doi.org/10.1128/msystems.00017-20 |