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Genetic dissection of centromere function.
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| Zeitschriftentitel: | Molecular and Cellular Biology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Molecular and Cellular Biology, 13, 1993, 6, S. 3156-3166 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Informa UK Limited
|
| Schlagwörter: |
| author_facet |
Schulman, I G Bloom, K Schulman, I G Bloom, K |
|---|---|
| author |
Schulman, I G Bloom, K |
| spellingShingle |
Schulman, I G Bloom, K Molecular and Cellular Biology Genetic dissection of centromere function. Cell Biology Molecular Biology |
| author_sort |
schulman, i g |
| spelling |
Schulman, I G Bloom, K 0270-7306 1098-5549 Informa UK Limited Cell Biology Molecular Biology http://dx.doi.org/10.1128/mcb.13.6.3156 <jats:p>A system to detect a minimal function of Saccharomyces cerevisiae centromeres in vivo has been developed. Centromere DNA mutants have been examined and found to be active in a plasmid copy number control assay in the absence of segregation. The experiments allow the identification of a minimal centromere unit, CDE III, independently of its ability to mediate chromosome segregation. Centromere-mediated plasmid copy number control correlates with the ability of CDE III to assemble a DNA-protein complex. Cells forced to maintain excess copies of CDE III exhibit increased loss of a nonessential artificial chromosome. Thus, segregationally impaired centromeres can have negative effects in trans on chromosome segregation. The use of a plasmid copy number control assay has allowed assembly steps preceding chromosome segregation to be defined.</jats:p> Genetic dissection of centromere function. Molecular and Cellular Biology |
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Informa UK Limited, 1993 |
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Informa UK Limited, 1993 |
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1993 |
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Molecular and Cellular Biology |
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49 |
| title |
Genetic dissection of centromere function. |
| title_unstemmed |
Genetic dissection of centromere function. |
| title_full |
Genetic dissection of centromere function. |
| title_fullStr |
Genetic dissection of centromere function. |
| title_full_unstemmed |
Genetic dissection of centromere function. |
| title_short |
Genetic dissection of centromere function. |
| title_sort |
genetic dissection of centromere function. |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1128/mcb.13.6.3156 |
| publishDate |
1993 |
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3156-3166 |
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<jats:p>A system to detect a minimal function of Saccharomyces cerevisiae centromeres in vivo has been developed. Centromere DNA mutants have been examined and found to be active in a plasmid copy number control assay in the absence of segregation. The experiments allow the identification of a minimal centromere unit, CDE III, independently of its ability to mediate chromosome segregation. Centromere-mediated plasmid copy number control correlates with the ability of CDE III to assemble a DNA-protein complex. Cells forced to maintain excess copies of CDE III exhibit increased loss of a nonessential artificial chromosome. Thus, segregationally impaired centromeres can have negative effects in trans on chromosome segregation. The use of a plasmid copy number control assay has allowed assembly steps preceding chromosome segregation to be defined.</jats:p> |
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| description | <jats:p>A system to detect a minimal function of Saccharomyces cerevisiae centromeres in vivo has been developed. Centromere DNA mutants have been examined and found to be active in a plasmid copy number control assay in the absence of segregation. The experiments allow the identification of a minimal centromere unit, CDE III, independently of its ability to mediate chromosome segregation. Centromere-mediated plasmid copy number control correlates with the ability of CDE III to assemble a DNA-protein complex. Cells forced to maintain excess copies of CDE III exhibit increased loss of a nonessential artificial chromosome. Thus, segregationally impaired centromeres can have negative effects in trans on chromosome segregation. The use of a plasmid copy number control assay has allowed assembly steps preceding chromosome segregation to be defined.</jats:p> |
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| imprint | Informa UK Limited, 1993 |
| imprint_str_mv | Informa UK Limited, 1993 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| series | Molecular and Cellular Biology |
| source_id | 49 |
| spelling | Schulman, I G Bloom, K 0270-7306 1098-5549 Informa UK Limited Cell Biology Molecular Biology http://dx.doi.org/10.1128/mcb.13.6.3156 <jats:p>A system to detect a minimal function of Saccharomyces cerevisiae centromeres in vivo has been developed. Centromere DNA mutants have been examined and found to be active in a plasmid copy number control assay in the absence of segregation. The experiments allow the identification of a minimal centromere unit, CDE III, independently of its ability to mediate chromosome segregation. Centromere-mediated plasmid copy number control correlates with the ability of CDE III to assemble a DNA-protein complex. Cells forced to maintain excess copies of CDE III exhibit increased loss of a nonessential artificial chromosome. Thus, segregationally impaired centromeres can have negative effects in trans on chromosome segregation. The use of a plasmid copy number control assay has allowed assembly steps preceding chromosome segregation to be defined.</jats:p> Genetic dissection of centromere function. Molecular and Cellular Biology |
| spellingShingle | Schulman, I G, Bloom, K, Molecular and Cellular Biology, Genetic dissection of centromere function., Cell Biology, Molecular Biology |
| title | Genetic dissection of centromere function. |
| title_full | Genetic dissection of centromere function. |
| title_fullStr | Genetic dissection of centromere function. |
| title_full_unstemmed | Genetic dissection of centromere function. |
| title_short | Genetic dissection of centromere function. |
| title_sort | genetic dissection of centromere function. |
| title_unstemmed | Genetic dissection of centromere function. |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1128/mcb.13.6.3156 |