Eintrag weiter verarbeiten
Structural proteins of adenovirus-associated virus: subspecies and their relatedness
Gespeichert in:
| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Virology, 46, 1983, 2, S. 523-529 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
McPherson, R A Rose, J A McPherson, R A Rose, J A |
|---|---|
| author |
McPherson, R A Rose, J A |
| spellingShingle |
McPherson, R A Rose, J A Journal of Virology Structural proteins of adenovirus-associated virus: subspecies and their relatedness Virology Insect Science Immunology Microbiology |
| author_sort |
mcpherson, r a |
| spelling |
McPherson, R A Rose, J A 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.46.2.523-529.1983 <jats:p>Capsids of adenovirus-associated virus (AAV) are known to contain three major structural proteins (A, B, and C). We have further resolved distinct subspecies of two of the major AAV proteins (two forms of protein A and four forms of protein C) which were found in both AAV1 and AAV2 serotypes. All subspecies were accurately synthesized in a cell-free translation system programmed with RNA isolated from infected cells. Analysis of virion proteins from the autonomous parvovirus H1 did not reveal a comparable array of subspecies of its major components. Staphylococcal V8 protease digestion of C proteins from AAV1 and AAV2 yielded very different electrophoretic patterns, indicating a considerable difference between the C proteins of these two serotypes, despite a high degree of genomic homology and an overall similarity in the number and relative proportions of analogous capsid proteins. On the other hand, staphylococcal V8 protease digestion of isolated proteins A, B, and C of AAV2 showed an extensive overlap among these proteins, possibly equivalent to all of protein C. In conjunction with other data, these findings suggest that proteins A, B, and C arise from different in-frame initiation sites contained in mRNA sequences that are transcribed from the right half of the AAV genome. The heterogeneity of subspecies may be explained by a partial read through of several tandem termination codons near the 3' end of AAV mRNA.</jats:p> Structural proteins of adenovirus-associated virus: subspecies and their relatedness Journal of Virology |
| doi_str_mv |
10.1128/jvi.46.2.523-529.1983 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNDYuMi41MjMtNTI5LjE5ODM |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNDYuMi41MjMtNTI5LjE5ODM |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
American Society for Microbiology, 1983 |
| imprint_str_mv |
American Society for Microbiology, 1983 |
| issn |
1098-5514 0022-538X |
| issn_str_mv |
1098-5514 0022-538X |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
mcpherson1983structuralproteinsofadenovirusassociatedvirussubspeciesandtheirrelatedness |
| publishDateSort |
1983 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Virology |
| source_id |
49 |
| title |
Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_unstemmed |
Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_full |
Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_fullStr |
Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_full_unstemmed |
Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_short |
Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_sort |
structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| topic |
Virology Insect Science Immunology Microbiology |
| url |
http://dx.doi.org/10.1128/jvi.46.2.523-529.1983 |
| publishDate |
1983 |
| physical |
523-529 |
| description |
<jats:p>Capsids of adenovirus-associated virus (AAV) are known to contain three major structural proteins (A, B, and C). We have further resolved distinct subspecies of two of the major AAV proteins (two forms of protein A and four forms of protein C) which were found in both AAV1 and AAV2 serotypes. All subspecies were accurately synthesized in a cell-free translation system programmed with RNA isolated from infected cells. Analysis of virion proteins from the autonomous parvovirus H1 did not reveal a comparable array of subspecies of its major components. Staphylococcal V8 protease digestion of C proteins from AAV1 and AAV2 yielded very different electrophoretic patterns, indicating a considerable difference between the C proteins of these two serotypes, despite a high degree of genomic homology and an overall similarity in the number and relative proportions of analogous capsid proteins. On the other hand, staphylococcal V8 protease digestion of isolated proteins A, B, and C of AAV2 showed an extensive overlap among these proteins, possibly equivalent to all of protein C. In conjunction with other data, these findings suggest that proteins A, B, and C arise from different in-frame initiation sites contained in mRNA sequences that are transcribed from the right half of the AAV genome. The heterogeneity of subspecies may be explained by a partial read through of several tandem termination codons near the 3' end of AAV mRNA.</jats:p> |
| container_issue |
2 |
| container_start_page |
523 |
| container_title |
Journal of Virology |
| container_volume |
46 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792330663561527302 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T13:28:39.192Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Structural+proteins+of+adenovirus-associated+virus%3A+subspecies+and+their+relatedness&rft.date=1983-05-01&genre=article&issn=1098-5514&volume=46&issue=2&spage=523&epage=529&pages=523-529&jtitle=Journal+of+Virology&atitle=Structural+proteins+of+adenovirus-associated+virus%3A+subspecies+and+their+relatedness&aulast=Rose&aufirst=J+A&rft_id=info%3Adoi%2F10.1128%2Fjvi.46.2.523-529.1983&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792330663561527302 |
| author | McPherson, R A, Rose, J A |
| author_facet | McPherson, R A, Rose, J A, McPherson, R A, Rose, J A |
| author_sort | mcpherson, r a |
| container_issue | 2 |
| container_start_page | 523 |
| container_title | Journal of Virology |
| container_volume | 46 |
| description | <jats:p>Capsids of adenovirus-associated virus (AAV) are known to contain three major structural proteins (A, B, and C). We have further resolved distinct subspecies of two of the major AAV proteins (two forms of protein A and four forms of protein C) which were found in both AAV1 and AAV2 serotypes. All subspecies were accurately synthesized in a cell-free translation system programmed with RNA isolated from infected cells. Analysis of virion proteins from the autonomous parvovirus H1 did not reveal a comparable array of subspecies of its major components. Staphylococcal V8 protease digestion of C proteins from AAV1 and AAV2 yielded very different electrophoretic patterns, indicating a considerable difference between the C proteins of these two serotypes, despite a high degree of genomic homology and an overall similarity in the number and relative proportions of analogous capsid proteins. On the other hand, staphylococcal V8 protease digestion of isolated proteins A, B, and C of AAV2 showed an extensive overlap among these proteins, possibly equivalent to all of protein C. In conjunction with other data, these findings suggest that proteins A, B, and C arise from different in-frame initiation sites contained in mRNA sequences that are transcribed from the right half of the AAV genome. The heterogeneity of subspecies may be explained by a partial read through of several tandem termination codons near the 3' end of AAV mRNA.</jats:p> |
| doi_str_mv | 10.1128/jvi.46.2.523-529.1983 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin, Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNDYuMi41MjMtNTI5LjE5ODM |
| imprint | American Society for Microbiology, 1983 |
| imprint_str_mv | American Society for Microbiology, 1983 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 1098-5514, 0022-538X |
| issn_str_mv | 1098-5514, 0022-538X |
| language | English |
| last_indexed | 2024-03-01T13:28:39.192Z |
| match_str | mcpherson1983structuralproteinsofadenovirusassociatedvirussubspeciesandtheirrelatedness |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 523-529 |
| publishDate | 1983 |
| publishDateSort | 1983 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Virology |
| source_id | 49 |
| spelling | McPherson, R A Rose, J A 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.46.2.523-529.1983 <jats:p>Capsids of adenovirus-associated virus (AAV) are known to contain three major structural proteins (A, B, and C). We have further resolved distinct subspecies of two of the major AAV proteins (two forms of protein A and four forms of protein C) which were found in both AAV1 and AAV2 serotypes. All subspecies were accurately synthesized in a cell-free translation system programmed with RNA isolated from infected cells. Analysis of virion proteins from the autonomous parvovirus H1 did not reveal a comparable array of subspecies of its major components. Staphylococcal V8 protease digestion of C proteins from AAV1 and AAV2 yielded very different electrophoretic patterns, indicating a considerable difference between the C proteins of these two serotypes, despite a high degree of genomic homology and an overall similarity in the number and relative proportions of analogous capsid proteins. On the other hand, staphylococcal V8 protease digestion of isolated proteins A, B, and C of AAV2 showed an extensive overlap among these proteins, possibly equivalent to all of protein C. In conjunction with other data, these findings suggest that proteins A, B, and C arise from different in-frame initiation sites contained in mRNA sequences that are transcribed from the right half of the AAV genome. The heterogeneity of subspecies may be explained by a partial read through of several tandem termination codons near the 3' end of AAV mRNA.</jats:p> Structural proteins of adenovirus-associated virus: subspecies and their relatedness Journal of Virology |
| spellingShingle | McPherson, R A, Rose, J A, Journal of Virology, Structural proteins of adenovirus-associated virus: subspecies and their relatedness, Virology, Insect Science, Immunology, Microbiology |
| title | Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_full | Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_fullStr | Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_full_unstemmed | Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_short | Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_sort | structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| title_unstemmed | Structural proteins of adenovirus-associated virus: subspecies and their relatedness |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.46.2.523-529.1983 |