Eintrag weiter verarbeiten
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2
Gespeichert in:
| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Virology, 37, 1981, 3, S. 1051-1059 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Colberg-Poley, A M Isom, H C Rapp, F Colberg-Poley, A M Isom, H C Rapp, F |
|---|---|
| author |
Colberg-Poley, A M Isom, H C Rapp, F |
| spellingShingle |
Colberg-Poley, A M Isom, H C Rapp, F Journal of Virology Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 Virology Insect Science Immunology Microbiology |
| author_sort |
colberg-poley, a m |
| spelling |
Colberg-Poley, A M Isom, H C Rapp, F 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.37.3.1051-1059.1981 <jats:p>We have previously described an in vitro system in which the function lacking for herpes simplex virus type 2 (HSV-2) replication can be induced by human cytomegalovirus (HCMV). The mechanism of this reactivation of quiescent HSV-2 by HCMV has been further defined. The HCMV function(s) responsible for HSV-2 stimulation was examined temporally, and the fraction of cells in quiescent cultures producing HSV-2 after superinfection was determined. Using independent biological, genetic and molecular techniques we have made the following observations. (i) As early as 12 h after HCMV superinfection, HSV-2 RNA was expressed in latently infected cells. (ii) At 24 h after HCMV superinfection, a time when newly synthesized HCMV was not yet apparent, infectious HSV-2 was produced by reactivated cultures. (iii) Four HCMV temperature-sensitive mutants, which are DNA-negative at nonpermissive temperature and represent four different complementation groups, induced reactivation of HSV-2 at 39.5 degrees C. (iv) Early after HCMV superinfection, 1.6% of quiescent cells could be induced to transcribe HSV-2 information. (v) Early after HCMV superinfection, 0.3% of cells in the quiescent cultures could be induced to yield infectious HSV-2. The finding that a significant interaction can occur between HCMV and quiescent HSV-2 in an in vitro model is noteworthy in light of the knowledge that both of these herpesviruses often reside simultaneously in the human host.</jats:p> Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 Journal of Virology |
| doi_str_mv |
10.1128/jvi.37.3.1051-1059.1981 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuMzcuMy4xMDUxLTEwNTkuMTk4MQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuMzcuMy4xMDUxLTEwNTkuMTk4MQ |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
American Society for Microbiology, 1981 |
| imprint_str_mv |
American Society for Microbiology, 1981 |
| issn |
0022-538X 1098-5514 |
| issn_str_mv |
0022-538X 1098-5514 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
colbergpoley1981involvementofanearlyhumancytomegalovirusfunctioninreactivationofquiescentherpessimplexvirustype2 |
| publishDateSort |
1981 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Virology |
| source_id |
49 |
| title |
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_unstemmed |
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_full |
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_fullStr |
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_full_unstemmed |
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_short |
Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_sort |
involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| topic |
Virology Insect Science Immunology Microbiology |
| url |
http://dx.doi.org/10.1128/jvi.37.3.1051-1059.1981 |
| publishDate |
1981 |
| physical |
1051-1059 |
| description |
<jats:p>We have previously described an in vitro system in which the function lacking for herpes simplex virus type 2 (HSV-2) replication can be induced by human cytomegalovirus (HCMV). The mechanism of this reactivation of quiescent HSV-2 by HCMV has been further defined. The HCMV function(s) responsible for HSV-2 stimulation was examined temporally, and the fraction of cells in quiescent cultures producing HSV-2 after superinfection was determined. Using independent biological, genetic and molecular techniques we have made the following observations. (i) As early as 12 h after HCMV superinfection, HSV-2 RNA was expressed in latently infected cells. (ii) At 24 h after HCMV superinfection, a time when newly synthesized HCMV was not yet apparent, infectious HSV-2 was produced by reactivated cultures. (iii) Four HCMV temperature-sensitive mutants, which are DNA-negative at nonpermissive temperature and represent four different complementation groups, induced reactivation of HSV-2 at 39.5 degrees C. (iv) Early after HCMV superinfection, 1.6% of quiescent cells could be induced to transcribe HSV-2 information. (v) Early after HCMV superinfection, 0.3% of cells in the quiescent cultures could be induced to yield infectious HSV-2. The finding that a significant interaction can occur between HCMV and quiescent HSV-2 in an in vitro model is noteworthy in light of the knowledge that both of these herpesviruses often reside simultaneously in the human host.</jats:p> |
| container_issue |
3 |
| container_start_page |
1051 |
| container_title |
Journal of Virology |
| container_volume |
37 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792343123831029767 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:46:24.278Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Involvement+of+an+early+human+cytomegalovirus+function+in+reactivation+of+quiescent+herpes+simplex+virus+type+2&rft.date=1981-03-01&genre=article&issn=1098-5514&volume=37&issue=3&spage=1051&epage=1059&pages=1051-1059&jtitle=Journal+of+Virology&atitle=Involvement+of+an+early+human+cytomegalovirus+function+in+reactivation+of+quiescent+herpes+simplex+virus+type+2&aulast=Rapp&aufirst=F&rft_id=info%3Adoi%2F10.1128%2Fjvi.37.3.1051-1059.1981&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792343123831029767 |
| author | Colberg-Poley, A M, Isom, H C, Rapp, F |
| author_facet | Colberg-Poley, A M, Isom, H C, Rapp, F, Colberg-Poley, A M, Isom, H C, Rapp, F |
| author_sort | colberg-poley, a m |
| container_issue | 3 |
| container_start_page | 1051 |
| container_title | Journal of Virology |
| container_volume | 37 |
| description | <jats:p>We have previously described an in vitro system in which the function lacking for herpes simplex virus type 2 (HSV-2) replication can be induced by human cytomegalovirus (HCMV). The mechanism of this reactivation of quiescent HSV-2 by HCMV has been further defined. The HCMV function(s) responsible for HSV-2 stimulation was examined temporally, and the fraction of cells in quiescent cultures producing HSV-2 after superinfection was determined. Using independent biological, genetic and molecular techniques we have made the following observations. (i) As early as 12 h after HCMV superinfection, HSV-2 RNA was expressed in latently infected cells. (ii) At 24 h after HCMV superinfection, a time when newly synthesized HCMV was not yet apparent, infectious HSV-2 was produced by reactivated cultures. (iii) Four HCMV temperature-sensitive mutants, which are DNA-negative at nonpermissive temperature and represent four different complementation groups, induced reactivation of HSV-2 at 39.5 degrees C. (iv) Early after HCMV superinfection, 1.6% of quiescent cells could be induced to transcribe HSV-2 information. (v) Early after HCMV superinfection, 0.3% of cells in the quiescent cultures could be induced to yield infectious HSV-2. The finding that a significant interaction can occur between HCMV and quiescent HSV-2 in an in vitro model is noteworthy in light of the knowledge that both of these herpesviruses often reside simultaneously in the human host.</jats:p> |
| doi_str_mv | 10.1128/jvi.37.3.1051-1059.1981 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin, Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuMzcuMy4xMDUxLTEwNTkuMTk4MQ |
| imprint | American Society for Microbiology, 1981 |
| imprint_str_mv | American Society for Microbiology, 1981 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0022-538X, 1098-5514 |
| issn_str_mv | 0022-538X, 1098-5514 |
| language | English |
| last_indexed | 2024-03-01T16:46:24.278Z |
| match_str | colbergpoley1981involvementofanearlyhumancytomegalovirusfunctioninreactivationofquiescentherpessimplexvirustype2 |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 1051-1059 |
| publishDate | 1981 |
| publishDateSort | 1981 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Virology |
| source_id | 49 |
| spelling | Colberg-Poley, A M Isom, H C Rapp, F 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.37.3.1051-1059.1981 <jats:p>We have previously described an in vitro system in which the function lacking for herpes simplex virus type 2 (HSV-2) replication can be induced by human cytomegalovirus (HCMV). The mechanism of this reactivation of quiescent HSV-2 by HCMV has been further defined. The HCMV function(s) responsible for HSV-2 stimulation was examined temporally, and the fraction of cells in quiescent cultures producing HSV-2 after superinfection was determined. Using independent biological, genetic and molecular techniques we have made the following observations. (i) As early as 12 h after HCMV superinfection, HSV-2 RNA was expressed in latently infected cells. (ii) At 24 h after HCMV superinfection, a time when newly synthesized HCMV was not yet apparent, infectious HSV-2 was produced by reactivated cultures. (iii) Four HCMV temperature-sensitive mutants, which are DNA-negative at nonpermissive temperature and represent four different complementation groups, induced reactivation of HSV-2 at 39.5 degrees C. (iv) Early after HCMV superinfection, 1.6% of quiescent cells could be induced to transcribe HSV-2 information. (v) Early after HCMV superinfection, 0.3% of cells in the quiescent cultures could be induced to yield infectious HSV-2. The finding that a significant interaction can occur between HCMV and quiescent HSV-2 in an in vitro model is noteworthy in light of the knowledge that both of these herpesviruses often reside simultaneously in the human host.</jats:p> Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 Journal of Virology |
| spellingShingle | Colberg-Poley, A M, Isom, H C, Rapp, F, Journal of Virology, Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2, Virology, Insect Science, Immunology, Microbiology |
| title | Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_full | Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_fullStr | Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_full_unstemmed | Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_short | Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_sort | involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| title_unstemmed | Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2 |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.37.3.1051-1059.1981 |