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Ginsberg, H S
Oosterom-Dragon, E A
Ginsberg, H S
author Oosterom-Dragon, E A
Ginsberg, H S
spellingShingle Oosterom-Dragon, E A
Ginsberg, H S
Journal of Virology
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
Virology
Insect Science
Immunology
Microbiology
author_sort oosterom-dragon, e a
spelling Oosterom-Dragon, E A Ginsberg, H S 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980 <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p> Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein Journal of Virology
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title Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_unstemmed Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_full Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_fullStr Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_full_unstemmed Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_short Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_sort purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
topic Virology
Insect Science
Immunology
Microbiology
url http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980
publishDate 1980
physical 1203-1207
description <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p>
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author Oosterom-Dragon, E A, Ginsberg, H S
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description <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p>
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spelling Oosterom-Dragon, E A Ginsberg, H S 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980 <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p> Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein Journal of Virology
spellingShingle Oosterom-Dragon, E A, Ginsberg, H S, Journal of Virology, Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein, Virology, Insect Science, Immunology, Microbiology
title Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_full Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_fullStr Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_full_unstemmed Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_short Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_sort purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
title_unstemmed Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
topic Virology, Insect Science, Immunology, Microbiology
url http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980