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Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein
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Zeitschriftentitel: | Journal of Virology |
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Personen und Körperschaften: | , |
In: | Journal of Virology, 33, 1980, 3, S. 1203-1207 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
American Society for Microbiology
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Schlagwörter: |
author_facet |
Oosterom-Dragon, E A Ginsberg, H S Oosterom-Dragon, E A Ginsberg, H S |
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author |
Oosterom-Dragon, E A Ginsberg, H S |
spellingShingle |
Oosterom-Dragon, E A Ginsberg, H S Journal of Virology Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein Virology Insect Science Immunology Microbiology |
author_sort |
oosterom-dragon, e a |
spelling |
Oosterom-Dragon, E A Ginsberg, H S 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980 <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p> Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein Journal of Virology |
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10.1128/jvi.33.3.1203-1207.1980 |
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American Society for Microbiology, 1980 |
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American Society for Microbiology, 1980 |
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1980 |
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American Society for Microbiology |
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Journal of Virology |
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49 |
title |
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_unstemmed |
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_full |
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_fullStr |
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_full_unstemmed |
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_short |
Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_sort |
purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
topic |
Virology Insect Science Immunology Microbiology |
url |
http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980 |
publishDate |
1980 |
physical |
1203-1207 |
description |
<jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p> |
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author | Oosterom-Dragon, E A, Ginsberg, H S |
author_facet | Oosterom-Dragon, E A, Ginsberg, H S, Oosterom-Dragon, E A, Ginsberg, H S |
author_sort | oosterom-dragon, e a |
container_issue | 3 |
container_start_page | 1203 |
container_title | Journal of Virology |
container_volume | 33 |
description | <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p> |
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imprint | American Society for Microbiology, 1980 |
imprint_str_mv | American Society for Microbiology, 1980 |
institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
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publisher | American Society for Microbiology |
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series | Journal of Virology |
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spelling | Oosterom-Dragon, E A Ginsberg, H S 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980 <jats:p>The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.</jats:p> Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein Journal of Virology |
spellingShingle | Oosterom-Dragon, E A, Ginsberg, H S, Journal of Virology, Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein, Virology, Insect Science, Immunology, Microbiology |
title | Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_full | Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_fullStr | Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_full_unstemmed | Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_short | Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_sort | purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
title_unstemmed | Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein |
topic | Virology, Insect Science, Immunology, Microbiology |
url | http://dx.doi.org/10.1128/jvi.33.3.1203-1207.1980 |