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Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Bacteriology, 188, 2006, 7, S. 2578-2585 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Zalieckas, Jill M. Wray, Lewis V. Fisher, Susan H. Zalieckas, Jill M. Wray, Lewis V. Fisher, Susan H. |
|---|---|
| author |
Zalieckas, Jill M. Wray, Lewis V. Fisher, Susan H. |
| spellingShingle |
Zalieckas, Jill M. Wray, Lewis V. Fisher, Susan H. Journal of Bacteriology Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA Molecular Biology Microbiology |
| author_sort |
zalieckas, jill m. |
| spelling |
Zalieckas, Jill M. Wray, Lewis V. Fisher, Susan H. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.188.7.2578-2585.2006 <jats:title>ABSTRACT</jats:title> <jats:p> Two <jats:italic>Bacillus subtilis</jats:italic> transcriptional factors, TnrA and GlnR, regulate gene expression in response to changes in nitrogen availability. These two proteins have similar amino acid sequences in their DNA binding domains and bind to DNA sites (GlnR/TnrA sites) that have the same consensus sequence. Expression of the <jats:italic>tnrA</jats:italic> gene was found to be activated by TnrA and repressed by GlnR. Mutational analysis demonstrated that a GlnR/TnrA site which lies immediately upstream of the −35 region of the <jats:italic>tnrA</jats:italic> promoter is required for regulation of <jats:italic>tnrA</jats:italic> expression by both GlnR and TnrA. Expression of the <jats:italic>glnRA</jats:italic> operon, which contains two GlnR/TnrA binding sites ( <jats:italic>glnRAo1</jats:italic> and <jats:italic>glnRAo2</jats:italic> ) in its promoter region, is repressed by both GlnR and TnrA. The <jats:italic>glnRAo2</jats:italic> site, which overlaps the −35 region of the <jats:italic>glnRA</jats:italic> promoter, was shown to be required for regulation by both GlnR and TnrA, while the <jats:italic>glnRAo1</jats:italic> site which lies upstream of the −35 promoter region is only involved in GlnR-mediated regulation. Examination of TnrA binding to <jats:italic>tnrA</jats:italic> and <jats:italic>glnRA</jats:italic> promoter DNA in gel mobility shift experiments showed that TnrA bound with an equilibrium dissociation binding constant of 55 nM to the GlnR/TnrA site in the <jats:italic>tnrA</jats:italic> promoter region, while the affinities of TnrA for the two GlnR/TnrA sites in the <jats:italic>glnRA</jats:italic> promoter region were greater than 3 μM. These results demonstrate that GlnR and TnrA cross-regulate each other's expression and that there are differences in their DNA-binding specificities. </jats:p> Cross-Regulation of the <i>Bacillus subtilis glnRA</i> and <i>tnrA</i> Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA Journal of Bacteriology |
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American Society for Microbiology, 2006 |
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American Society for Microbiology, 2006 |
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0021-9193 1098-5530 |
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2006 |
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American Society for Microbiology |
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Journal of Bacteriology |
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49 |
| title |
Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_unstemmed |
Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_full |
Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_fullStr |
Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_full_unstemmed |
Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_short |
Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_sort |
cross-regulation of the
<i>bacillus subtilis glnra</i>
and
<i>tnra</i>
genes provides evidence for dna binding site discrimination by glnr and tnra |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.188.7.2578-2585.2006 |
| publishDate |
2006 |
| physical |
2578-2585 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Two
<jats:italic>Bacillus subtilis</jats:italic>
transcriptional factors, TnrA and GlnR, regulate gene expression in response to changes in nitrogen availability. These two proteins have similar amino acid sequences in their DNA binding domains and bind to DNA sites (GlnR/TnrA sites) that have the same consensus sequence. Expression of the
<jats:italic>tnrA</jats:italic>
gene was found to be activated by TnrA and repressed by GlnR. Mutational analysis demonstrated that a GlnR/TnrA site which lies immediately upstream of the −35 region of the
<jats:italic>tnrA</jats:italic>
promoter is required for regulation of
<jats:italic>tnrA</jats:italic>
expression by both GlnR and TnrA. Expression of the
<jats:italic>glnRA</jats:italic>
operon, which contains two GlnR/TnrA binding sites (
<jats:italic>glnRAo1</jats:italic>
and
<jats:italic>glnRAo2</jats:italic>
) in its promoter region, is repressed by both GlnR and TnrA. The
<jats:italic>glnRAo2</jats:italic>
site, which overlaps the −35 region of the
<jats:italic>glnRA</jats:italic>
promoter, was shown to be required for regulation by both GlnR and TnrA, while the
<jats:italic>glnRAo1</jats:italic>
site which lies upstream of the −35 promoter region is only involved in GlnR-mediated regulation. Examination of TnrA binding to
<jats:italic>tnrA</jats:italic>
and
<jats:italic>glnRA</jats:italic>
promoter DNA in gel mobility shift experiments showed that TnrA bound with an equilibrium dissociation binding constant of 55 nM to the GlnR/TnrA site in the
<jats:italic>tnrA</jats:italic>
promoter region, while the affinities of TnrA for the two GlnR/TnrA sites in the
<jats:italic>glnRA</jats:italic>
promoter region were greater than 3 μM. These results demonstrate that GlnR and TnrA cross-regulate each other's expression and that there are differences in their DNA-binding specificities.
</jats:p> |
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| author | Zalieckas, Jill M., Wray, Lewis V., Fisher, Susan H. |
| author_facet | Zalieckas, Jill M., Wray, Lewis V., Fisher, Susan H., Zalieckas, Jill M., Wray, Lewis V., Fisher, Susan H. |
| author_sort | zalieckas, jill m. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Two <jats:italic>Bacillus subtilis</jats:italic> transcriptional factors, TnrA and GlnR, regulate gene expression in response to changes in nitrogen availability. These two proteins have similar amino acid sequences in their DNA binding domains and bind to DNA sites (GlnR/TnrA sites) that have the same consensus sequence. Expression of the <jats:italic>tnrA</jats:italic> gene was found to be activated by TnrA and repressed by GlnR. Mutational analysis demonstrated that a GlnR/TnrA site which lies immediately upstream of the −35 region of the <jats:italic>tnrA</jats:italic> promoter is required for regulation of <jats:italic>tnrA</jats:italic> expression by both GlnR and TnrA. Expression of the <jats:italic>glnRA</jats:italic> operon, which contains two GlnR/TnrA binding sites ( <jats:italic>glnRAo1</jats:italic> and <jats:italic>glnRAo2</jats:italic> ) in its promoter region, is repressed by both GlnR and TnrA. The <jats:italic>glnRAo2</jats:italic> site, which overlaps the −35 region of the <jats:italic>glnRA</jats:italic> promoter, was shown to be required for regulation by both GlnR and TnrA, while the <jats:italic>glnRAo1</jats:italic> site which lies upstream of the −35 promoter region is only involved in GlnR-mediated regulation. Examination of TnrA binding to <jats:italic>tnrA</jats:italic> and <jats:italic>glnRA</jats:italic> promoter DNA in gel mobility shift experiments showed that TnrA bound with an equilibrium dissociation binding constant of 55 nM to the GlnR/TnrA site in the <jats:italic>tnrA</jats:italic> promoter region, while the affinities of TnrA for the two GlnR/TnrA sites in the <jats:italic>glnRA</jats:italic> promoter region were greater than 3 μM. These results demonstrate that GlnR and TnrA cross-regulate each other's expression and that there are differences in their DNA-binding specificities. </jats:p> |
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| imprint | American Society for Microbiology, 2006 |
| imprint_str_mv | American Society for Microbiology, 2006 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| physical | 2578-2585 |
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| spelling | Zalieckas, Jill M. Wray, Lewis V. Fisher, Susan H. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.188.7.2578-2585.2006 <jats:title>ABSTRACT</jats:title> <jats:p> Two <jats:italic>Bacillus subtilis</jats:italic> transcriptional factors, TnrA and GlnR, regulate gene expression in response to changes in nitrogen availability. These two proteins have similar amino acid sequences in their DNA binding domains and bind to DNA sites (GlnR/TnrA sites) that have the same consensus sequence. Expression of the <jats:italic>tnrA</jats:italic> gene was found to be activated by TnrA and repressed by GlnR. Mutational analysis demonstrated that a GlnR/TnrA site which lies immediately upstream of the −35 region of the <jats:italic>tnrA</jats:italic> promoter is required for regulation of <jats:italic>tnrA</jats:italic> expression by both GlnR and TnrA. Expression of the <jats:italic>glnRA</jats:italic> operon, which contains two GlnR/TnrA binding sites ( <jats:italic>glnRAo1</jats:italic> and <jats:italic>glnRAo2</jats:italic> ) in its promoter region, is repressed by both GlnR and TnrA. The <jats:italic>glnRAo2</jats:italic> site, which overlaps the −35 region of the <jats:italic>glnRA</jats:italic> promoter, was shown to be required for regulation by both GlnR and TnrA, while the <jats:italic>glnRAo1</jats:italic> site which lies upstream of the −35 promoter region is only involved in GlnR-mediated regulation. Examination of TnrA binding to <jats:italic>tnrA</jats:italic> and <jats:italic>glnRA</jats:italic> promoter DNA in gel mobility shift experiments showed that TnrA bound with an equilibrium dissociation binding constant of 55 nM to the GlnR/TnrA site in the <jats:italic>tnrA</jats:italic> promoter region, while the affinities of TnrA for the two GlnR/TnrA sites in the <jats:italic>glnRA</jats:italic> promoter region were greater than 3 μM. These results demonstrate that GlnR and TnrA cross-regulate each other's expression and that there are differences in their DNA-binding specificities. </jats:p> Cross-Regulation of the <i>Bacillus subtilis glnRA</i> and <i>tnrA</i> Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA Journal of Bacteriology |
| spellingShingle | Zalieckas, Jill M., Wray, Lewis V., Fisher, Susan H., Journal of Bacteriology, Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA, Molecular Biology, Microbiology |
| title | Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_full | Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_fullStr | Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_full_unstemmed | Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_short | Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| title_sort | cross-regulation of the <i>bacillus subtilis glnra</i> and <i>tnra</i> genes provides evidence for dna binding site discrimination by glnr and tnra |
| title_unstemmed | Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.188.7.2578-2585.2006 |