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A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the...
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Bacteriology, 188, 2006, 2, S. 794-800 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Silva, Anisia J. Benitez, Jorge A. Silva, Anisia J. Benitez, Jorge A. |
|---|---|
| author |
Silva, Anisia J. Benitez, Jorge A. |
| spellingShingle |
Silva, Anisia J. Benitez, Jorge A. Journal of Bacteriology A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine Molecular Biology Microbiology |
| author_sort |
silva, anisia j. |
| spelling |
Silva, Anisia J. Benitez, Jorge A. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.188.2.794-800.2006 <jats:title>ABSTRACT</jats:title> <jats:p> We have constructed a relaxed mutant of El Tor biotype <jats:italic>Vibrio cholerae</jats:italic> strain C7258 by disruption of the RelA catalytic domain. The ability of the <jats:italic>V. cholerae</jats:italic> relaxed mutant to biosynthesize guanosine tetraphosphate and pentaphosphate was severely affected; the mutant showed a reduced growth rate in minimal medium that could be reversed by the addition of Casamino Acids, and it was thermosensitive. Contrary to published findings, the new <jats:italic>relA</jats:italic> mutant still produced significant cholera toxin and toxin-coregulated pilus. The <jats:italic>V. cholerae relA</jats:italic> mutant was motile, produced normal biofilms, and colonized the suckling mouse intestine. Our data suggest that levels of basal guanosine nucleotides pppGpp and ppGpp, rather than the availability of a stringent response, could influence expression of virulence factors, depending on strain and culture conditions. Production of hemagglutinin (HA)/protease, which requires HapR, RpoS, and the cyclic AMP receptor protein, was not strongly affected. Nevertheless, overexpression of RelA protein from an isopropyl-β- <jats:sc>d</jats:sc> -thiogalactopyranoside-inducible promoter posttranscriptionally diminished production of HA/protease. </jats:p> A <i>Vibrio cholerae</i> Relaxed ( <i>relA</i> ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine Journal of Bacteriology |
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| title |
A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_unstemmed |
A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_full |
A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_fullStr |
A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_full_unstemmed |
A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_short |
A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_sort |
a
<i>vibrio cholerae</i>
relaxed (
<i>rela</i>
) mutant expresses major virulence factors, exhibits biofilm formation and motility, and colonizes the suckling mouse intestine |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.188.2.794-800.2006 |
| publishDate |
2006 |
| physical |
794-800 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
We have constructed a relaxed mutant of El Tor biotype
<jats:italic>Vibrio cholerae</jats:italic>
strain C7258 by disruption of the RelA catalytic domain. The ability of the
<jats:italic>V. cholerae</jats:italic>
relaxed mutant to biosynthesize guanosine tetraphosphate and pentaphosphate was severely affected; the mutant showed a reduced growth rate in minimal medium that could be reversed by the addition of Casamino Acids, and it was thermosensitive. Contrary to published findings, the new
<jats:italic>relA</jats:italic>
mutant still produced significant cholera toxin and toxin-coregulated pilus. The
<jats:italic>V. cholerae relA</jats:italic>
mutant was motile, produced normal biofilms, and colonized the suckling mouse intestine. Our data suggest that levels of basal guanosine nucleotides pppGpp and ppGpp, rather than the availability of a stringent response, could influence expression of virulence factors, depending on strain and culture conditions. Production of hemagglutinin (HA)/protease, which requires HapR, RpoS, and the cyclic AMP receptor protein, was not strongly affected. Nevertheless, overexpression of RelA protein from an isopropyl-β-
<jats:sc>d</jats:sc>
-thiogalactopyranoside-inducible promoter posttranscriptionally diminished production of HA/protease.
</jats:p> |
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| author | Silva, Anisia J., Benitez, Jorge A. |
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| author_sort | silva, anisia j. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> We have constructed a relaxed mutant of El Tor biotype <jats:italic>Vibrio cholerae</jats:italic> strain C7258 by disruption of the RelA catalytic domain. The ability of the <jats:italic>V. cholerae</jats:italic> relaxed mutant to biosynthesize guanosine tetraphosphate and pentaphosphate was severely affected; the mutant showed a reduced growth rate in minimal medium that could be reversed by the addition of Casamino Acids, and it was thermosensitive. Contrary to published findings, the new <jats:italic>relA</jats:italic> mutant still produced significant cholera toxin and toxin-coregulated pilus. The <jats:italic>V. cholerae relA</jats:italic> mutant was motile, produced normal biofilms, and colonized the suckling mouse intestine. Our data suggest that levels of basal guanosine nucleotides pppGpp and ppGpp, rather than the availability of a stringent response, could influence expression of virulence factors, depending on strain and culture conditions. Production of hemagglutinin (HA)/protease, which requires HapR, RpoS, and the cyclic AMP receptor protein, was not strongly affected. Nevertheless, overexpression of RelA protein from an isopropyl-β- <jats:sc>d</jats:sc> -thiogalactopyranoside-inducible promoter posttranscriptionally diminished production of HA/protease. </jats:p> |
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| imprint | American Society for Microbiology, 2006 |
| imprint_str_mv | American Society for Microbiology, 2006 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 794-800 |
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| spelling | Silva, Anisia J. Benitez, Jorge A. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.188.2.794-800.2006 <jats:title>ABSTRACT</jats:title> <jats:p> We have constructed a relaxed mutant of El Tor biotype <jats:italic>Vibrio cholerae</jats:italic> strain C7258 by disruption of the RelA catalytic domain. The ability of the <jats:italic>V. cholerae</jats:italic> relaxed mutant to biosynthesize guanosine tetraphosphate and pentaphosphate was severely affected; the mutant showed a reduced growth rate in minimal medium that could be reversed by the addition of Casamino Acids, and it was thermosensitive. Contrary to published findings, the new <jats:italic>relA</jats:italic> mutant still produced significant cholera toxin and toxin-coregulated pilus. The <jats:italic>V. cholerae relA</jats:italic> mutant was motile, produced normal biofilms, and colonized the suckling mouse intestine. Our data suggest that levels of basal guanosine nucleotides pppGpp and ppGpp, rather than the availability of a stringent response, could influence expression of virulence factors, depending on strain and culture conditions. Production of hemagglutinin (HA)/protease, which requires HapR, RpoS, and the cyclic AMP receptor protein, was not strongly affected. Nevertheless, overexpression of RelA protein from an isopropyl-β- <jats:sc>d</jats:sc> -thiogalactopyranoside-inducible promoter posttranscriptionally diminished production of HA/protease. </jats:p> A <i>Vibrio cholerae</i> Relaxed ( <i>relA</i> ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine Journal of Bacteriology |
| spellingShingle | Silva, Anisia J., Benitez, Jorge A., Journal of Bacteriology, A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine, Molecular Biology, Microbiology |
| title | A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_full | A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_fullStr | A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_full_unstemmed | A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_short | A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| title_sort | a <i>vibrio cholerae</i> relaxed ( <i>rela</i> ) mutant expresses major virulence factors, exhibits biofilm formation and motility, and colonizes the suckling mouse intestine |
| title_unstemmed | A Vibrio cholerae Relaxed ( relA ) Mutant Expresses Major Virulence Factors, Exhibits Biofilm Formation and Motility, and Colonizes the Suckling Mouse Intestine |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.188.2.794-800.2006 |