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Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 187, 2005, 13, S. 4607-4614 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Kalyuzhnaya, Marina G. Korotkova, Natalia Crowther, Gregory Marx, Christopher J. Lidstrom, Mary E. Chistoserdova, Ludmila Kalyuzhnaya, Marina G. Korotkova, Natalia Crowther, Gregory Marx, Christopher J. Lidstrom, Mary E. Chistoserdova, Ludmila |
|---|---|
| author |
Kalyuzhnaya, Marina G. Korotkova, Natalia Crowther, Gregory Marx, Christopher J. Lidstrom, Mary E. Chistoserdova, Ludmila |
| spellingShingle |
Kalyuzhnaya, Marina G. Korotkova, Natalia Crowther, Gregory Marx, Christopher J. Lidstrom, Mary E. Chistoserdova, Ludmila Journal of Bacteriology Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights Molecular Biology Microbiology |
| author_sort |
kalyuzhnaya, marina g. |
| spelling |
Kalyuzhnaya, Marina G. Korotkova, Natalia Crowther, Gregory Marx, Christopher J. Lidstrom, Mary E. Chistoserdova, Ludmila 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.187.13.4607-4614.2005 <jats:title>ABSTRACT</jats:title> <jats:p> In this study, the occurrence and chromosomal clustering of genes encoding C <jats:sub>1</jats:sub> transfer reactions linked to tetrahydromethanopterin (H <jats:sub>4</jats:sub> MPT) were analyzed in a variety of proteobacteria and in representatives of the <jats:italic>Planctomycetes</jats:italic> via genomic analysis or via partial sequencing by cosmid walking. Although a tendency for clustering was found common for the genes of interest, significant variations in gene order and the degree of clustering were uncovered both between and within different groups of <jats:italic>Proteobacteria</jats:italic> and between <jats:italic>Proteobacteria</jats:italic> and <jats:italic>Planctomycetes</jats:italic> . Phylogenetic analyses suggested that the evolution of genes encoding H <jats:sub>4</jats:sub> MPT-linked reactions in <jats:italic>Proteobacteria</jats:italic> involved lateral transfers within <jats:italic>Proteobacteria</jats:italic> and possibly between <jats:italic>Proteobacteria</jats:italic> and other phyla. Gene cluster comparisons revealed a number of novel genes potentially involved in the C <jats:sub>1</jats:sub> transfer reactions, and these were analyzed by mutation and expression analyses. Four genes, a homolog of <jats:italic>pabB</jats:italic> , and three genes conserved between methanogenic <jats:italic>Archaea</jats:italic> and <jats:italic>Bacteria</jats:italic> possessing H <jats:sub>4</jats:sub> MPT-linked functions, <jats:italic>orfY</jats:italic> , <jats:italic>orf1</jats:italic> , and <jats:italic>afpA</jats:italic> were shown to be involved in formaldehyde oxidation/detoxification, as judged by specific mutant phenotypes. In particular, <jats:italic>pabB</jats:italic> contributes to the biosynthesis of <jats:italic>para</jats:italic> -aminobenzoic acid, a precursor of both tetrahydrofolate and H <jats:sub>4</jats:sub> MPT, and <jats:italic>afpA</jats:italic> apparently encodes a novel dihydromethanopterin reductase, based on mutant complementation experiments. </jats:p> Analysis of Gene Islands Involved in Methanopterin-Linked C <sub>1</sub> Transfer Reactions Reveals New Functions and Provides Evolutionary Insights Journal of Bacteriology |
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American Society for Microbiology, 2005 |
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2005 |
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American Society for Microbiology |
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Journal of Bacteriology |
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49 |
| title |
Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_unstemmed |
Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_full |
Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_fullStr |
Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_full_unstemmed |
Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_short |
Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_sort |
analysis of gene islands involved in methanopterin-linked c
<sub>1</sub>
transfer reactions reveals new functions and provides evolutionary insights |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.187.13.4607-4614.2005 |
| publishDate |
2005 |
| physical |
4607-4614 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
In this study, the occurrence and chromosomal clustering of genes encoding C
<jats:sub>1</jats:sub>
transfer reactions linked to tetrahydromethanopterin (H
<jats:sub>4</jats:sub>
MPT) were analyzed in a variety of proteobacteria and in representatives of the
<jats:italic>Planctomycetes</jats:italic>
via genomic analysis or via partial sequencing by cosmid walking. Although a tendency for clustering was found common for the genes of interest, significant variations in gene order and the degree of clustering were uncovered both between and within different groups of
<jats:italic>Proteobacteria</jats:italic>
and between
<jats:italic>Proteobacteria</jats:italic>
and
<jats:italic>Planctomycetes</jats:italic>
. Phylogenetic analyses suggested that the evolution of genes encoding H
<jats:sub>4</jats:sub>
MPT-linked reactions in
<jats:italic>Proteobacteria</jats:italic>
involved lateral transfers within
<jats:italic>Proteobacteria</jats:italic>
and possibly between
<jats:italic>Proteobacteria</jats:italic>
and other phyla. Gene cluster comparisons revealed a number of novel genes potentially involved in the C
<jats:sub>1</jats:sub>
transfer reactions, and these were analyzed by mutation and expression analyses. Four genes, a homolog of
<jats:italic>pabB</jats:italic>
, and three genes conserved between methanogenic
<jats:italic>Archaea</jats:italic>
and
<jats:italic>Bacteria</jats:italic>
possessing H
<jats:sub>4</jats:sub>
MPT-linked functions,
<jats:italic>orfY</jats:italic>
,
<jats:italic>orf1</jats:italic>
, and
<jats:italic>afpA</jats:italic>
were shown to be involved in formaldehyde oxidation/detoxification, as judged by specific mutant phenotypes. In particular,
<jats:italic>pabB</jats:italic>
contributes to the biosynthesis of
<jats:italic>para</jats:italic>
-aminobenzoic acid, a precursor of both tetrahydrofolate and H
<jats:sub>4</jats:sub>
MPT, and
<jats:italic>afpA</jats:italic>
apparently encodes a novel dihydromethanopterin reductase, based on mutant complementation experiments.
</jats:p> |
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| author | Kalyuzhnaya, Marina G., Korotkova, Natalia, Crowther, Gregory, Marx, Christopher J., Lidstrom, Mary E., Chistoserdova, Ludmila |
| author_facet | Kalyuzhnaya, Marina G., Korotkova, Natalia, Crowther, Gregory, Marx, Christopher J., Lidstrom, Mary E., Chistoserdova, Ludmila, Kalyuzhnaya, Marina G., Korotkova, Natalia, Crowther, Gregory, Marx, Christopher J., Lidstrom, Mary E., Chistoserdova, Ludmila |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> In this study, the occurrence and chromosomal clustering of genes encoding C <jats:sub>1</jats:sub> transfer reactions linked to tetrahydromethanopterin (H <jats:sub>4</jats:sub> MPT) were analyzed in a variety of proteobacteria and in representatives of the <jats:italic>Planctomycetes</jats:italic> via genomic analysis or via partial sequencing by cosmid walking. Although a tendency for clustering was found common for the genes of interest, significant variations in gene order and the degree of clustering were uncovered both between and within different groups of <jats:italic>Proteobacteria</jats:italic> and between <jats:italic>Proteobacteria</jats:italic> and <jats:italic>Planctomycetes</jats:italic> . Phylogenetic analyses suggested that the evolution of genes encoding H <jats:sub>4</jats:sub> MPT-linked reactions in <jats:italic>Proteobacteria</jats:italic> involved lateral transfers within <jats:italic>Proteobacteria</jats:italic> and possibly between <jats:italic>Proteobacteria</jats:italic> and other phyla. Gene cluster comparisons revealed a number of novel genes potentially involved in the C <jats:sub>1</jats:sub> transfer reactions, and these were analyzed by mutation and expression analyses. Four genes, a homolog of <jats:italic>pabB</jats:italic> , and three genes conserved between methanogenic <jats:italic>Archaea</jats:italic> and <jats:italic>Bacteria</jats:italic> possessing H <jats:sub>4</jats:sub> MPT-linked functions, <jats:italic>orfY</jats:italic> , <jats:italic>orf1</jats:italic> , and <jats:italic>afpA</jats:italic> were shown to be involved in formaldehyde oxidation/detoxification, as judged by specific mutant phenotypes. In particular, <jats:italic>pabB</jats:italic> contributes to the biosynthesis of <jats:italic>para</jats:italic> -aminobenzoic acid, a precursor of both tetrahydrofolate and H <jats:sub>4</jats:sub> MPT, and <jats:italic>afpA</jats:italic> apparently encodes a novel dihydromethanopterin reductase, based on mutant complementation experiments. </jats:p> |
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| spelling | Kalyuzhnaya, Marina G. Korotkova, Natalia Crowther, Gregory Marx, Christopher J. Lidstrom, Mary E. Chistoserdova, Ludmila 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.187.13.4607-4614.2005 <jats:title>ABSTRACT</jats:title> <jats:p> In this study, the occurrence and chromosomal clustering of genes encoding C <jats:sub>1</jats:sub> transfer reactions linked to tetrahydromethanopterin (H <jats:sub>4</jats:sub> MPT) were analyzed in a variety of proteobacteria and in representatives of the <jats:italic>Planctomycetes</jats:italic> via genomic analysis or via partial sequencing by cosmid walking. Although a tendency for clustering was found common for the genes of interest, significant variations in gene order and the degree of clustering were uncovered both between and within different groups of <jats:italic>Proteobacteria</jats:italic> and between <jats:italic>Proteobacteria</jats:italic> and <jats:italic>Planctomycetes</jats:italic> . Phylogenetic analyses suggested that the evolution of genes encoding H <jats:sub>4</jats:sub> MPT-linked reactions in <jats:italic>Proteobacteria</jats:italic> involved lateral transfers within <jats:italic>Proteobacteria</jats:italic> and possibly between <jats:italic>Proteobacteria</jats:italic> and other phyla. Gene cluster comparisons revealed a number of novel genes potentially involved in the C <jats:sub>1</jats:sub> transfer reactions, and these were analyzed by mutation and expression analyses. Four genes, a homolog of <jats:italic>pabB</jats:italic> , and three genes conserved between methanogenic <jats:italic>Archaea</jats:italic> and <jats:italic>Bacteria</jats:italic> possessing H <jats:sub>4</jats:sub> MPT-linked functions, <jats:italic>orfY</jats:italic> , <jats:italic>orf1</jats:italic> , and <jats:italic>afpA</jats:italic> were shown to be involved in formaldehyde oxidation/detoxification, as judged by specific mutant phenotypes. In particular, <jats:italic>pabB</jats:italic> contributes to the biosynthesis of <jats:italic>para</jats:italic> -aminobenzoic acid, a precursor of both tetrahydrofolate and H <jats:sub>4</jats:sub> MPT, and <jats:italic>afpA</jats:italic> apparently encodes a novel dihydromethanopterin reductase, based on mutant complementation experiments. </jats:p> Analysis of Gene Islands Involved in Methanopterin-Linked C <sub>1</sub> Transfer Reactions Reveals New Functions and Provides Evolutionary Insights Journal of Bacteriology |
| spellingShingle | Kalyuzhnaya, Marina G., Korotkova, Natalia, Crowther, Gregory, Marx, Christopher J., Lidstrom, Mary E., Chistoserdova, Ludmila, Journal of Bacteriology, Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights, Molecular Biology, Microbiology |
| title | Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_full | Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_fullStr | Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_full_unstemmed | Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_short | Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| title_sort | analysis of gene islands involved in methanopterin-linked c <sub>1</sub> transfer reactions reveals new functions and provides evolutionary insights |
| title_unstemmed | Analysis of Gene Islands Involved in Methanopterin-Linked C 1 Transfer Reactions Reveals New Functions and Provides Evolutionary Insights |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.187.13.4607-4614.2005 |