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Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in D...
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Bacteriology, 185, 2003, 10, S. 3036-3041 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Ruíz, Raquel Marqués, Silvia Ramos, Juan L. Ruíz, Raquel Marqués, Silvia Ramos, Juan L. |
|---|---|
| author |
Ruíz, Raquel Marqués, Silvia Ramos, Juan L. |
| spellingShingle |
Ruíz, Raquel Marqués, Silvia Ramos, Juan L. Journal of Bacteriology Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization Molecular Biology Microbiology |
| author_sort |
ruíz, raquel |
| spelling |
Ruíz, Raquel Marqués, Silvia Ramos, Juan L. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.185.10.3036-3041.2003 <jats:title>ABSTRACT</jats:title> <jats:p> Members of the AraC/XylS family of transcriptional regulators are usually organized in two domains: a conserved domain made up of 100 amino acids and frequently located at the C-terminal end, involved in DNA binding; and an N-terminal nonconserved domain involved in signal recognition, as is the case for regulators involved in the control of carbon metabolism (R. Tobes and J. L. Ramos, Nucleic Acids Res. <jats:bold>30:</jats:bold> 318-321, 2002). The XylS protein, which is extremely insoluble, controls expression of the <jats:italic>meta</jats:italic> -cleavage pathway for alkylbenzoate metabolism. We fused the N-terminal end of XylS to the maltose-binding protein (MBP) in vitro and found in glutaraldehyde cross-linking assays that the protein dimerized. Experiments with a chimeric N-terminal XylS linked to a ′LexA protein showed that the dimer was stabilized in the presence of alkylbenzoates. Sequence alignments with AraC and UreR allowed us to identify three residues, Leu193, Leu194, and Ile205, as potentially being involved in dimerization. Site-directed mutagenesis of XylS in which each of the above residues was replaced with Ala revealed that Leu193 and Leu194 were critical for activity and that a chimera in which LexA was linked to the N terminus of XylSLeu193Ala or XylSLeu194Ala was not functional. Dimerization of the chimeras MBP-N-XylSLeu193Ala and MBP-N-XylSLeu194Ala was not observed in cross-linking assays with glutaraldehyde. </jats:p> Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL <i>meta</i> -Cleavage Pathway, Are Involved in Dimerization Journal of Bacteriology |
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American Society for Microbiology, 2003 |
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| title |
Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_unstemmed |
Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_full |
Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_fullStr |
Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_full_unstemmed |
Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_short |
Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_sort |
leucines 193 and 194 at the n-terminal domain of the xyls protein, the positive transcriptional regulator of the tol
<i>meta</i>
-cleavage pathway, are involved in dimerization |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.185.10.3036-3041.2003 |
| publishDate |
2003 |
| physical |
3036-3041 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Members of the AraC/XylS family of transcriptional regulators are usually organized in two domains: a conserved domain made up of 100 amino acids and frequently located at the C-terminal end, involved in DNA binding; and an N-terminal nonconserved domain involved in signal recognition, as is the case for regulators involved in the control of carbon metabolism (R. Tobes and J. L. Ramos, Nucleic Acids Res.
<jats:bold>30:</jats:bold>
318-321, 2002). The XylS protein, which is extremely insoluble, controls expression of the
<jats:italic>meta</jats:italic>
-cleavage pathway for alkylbenzoate metabolism. We fused the N-terminal end of XylS to the maltose-binding protein (MBP) in vitro and found in glutaraldehyde cross-linking assays that the protein dimerized. Experiments with a chimeric N-terminal XylS linked to a ′LexA protein showed that the dimer was stabilized in the presence of alkylbenzoates. Sequence alignments with AraC and UreR allowed us to identify three residues, Leu193, Leu194, and Ile205, as potentially being involved in dimerization. Site-directed mutagenesis of XylS in which each of the above residues was replaced with Ala revealed that Leu193 and Leu194 were critical for activity and that a chimera in which LexA was linked to the N terminus of XylSLeu193Ala or XylSLeu194Ala was not functional. Dimerization of the chimeras MBP-N-XylSLeu193Ala and MBP-N-XylSLeu194Ala was not observed in cross-linking assays with glutaraldehyde.
</jats:p> |
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| author | Ruíz, Raquel, Marqués, Silvia, Ramos, Juan L. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Members of the AraC/XylS family of transcriptional regulators are usually organized in two domains: a conserved domain made up of 100 amino acids and frequently located at the C-terminal end, involved in DNA binding; and an N-terminal nonconserved domain involved in signal recognition, as is the case for regulators involved in the control of carbon metabolism (R. Tobes and J. L. Ramos, Nucleic Acids Res. <jats:bold>30:</jats:bold> 318-321, 2002). The XylS protein, which is extremely insoluble, controls expression of the <jats:italic>meta</jats:italic> -cleavage pathway for alkylbenzoate metabolism. We fused the N-terminal end of XylS to the maltose-binding protein (MBP) in vitro and found in glutaraldehyde cross-linking assays that the protein dimerized. Experiments with a chimeric N-terminal XylS linked to a ′LexA protein showed that the dimer was stabilized in the presence of alkylbenzoates. Sequence alignments with AraC and UreR allowed us to identify three residues, Leu193, Leu194, and Ile205, as potentially being involved in dimerization. Site-directed mutagenesis of XylS in which each of the above residues was replaced with Ala revealed that Leu193 and Leu194 were critical for activity and that a chimera in which LexA was linked to the N terminus of XylSLeu193Ala or XylSLeu194Ala was not functional. Dimerization of the chimeras MBP-N-XylSLeu193Ala and MBP-N-XylSLeu194Ala was not observed in cross-linking assays with glutaraldehyde. </jats:p> |
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| spelling | Ruíz, Raquel Marqués, Silvia Ramos, Juan L. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.185.10.3036-3041.2003 <jats:title>ABSTRACT</jats:title> <jats:p> Members of the AraC/XylS family of transcriptional regulators are usually organized in two domains: a conserved domain made up of 100 amino acids and frequently located at the C-terminal end, involved in DNA binding; and an N-terminal nonconserved domain involved in signal recognition, as is the case for regulators involved in the control of carbon metabolism (R. Tobes and J. L. Ramos, Nucleic Acids Res. <jats:bold>30:</jats:bold> 318-321, 2002). The XylS protein, which is extremely insoluble, controls expression of the <jats:italic>meta</jats:italic> -cleavage pathway for alkylbenzoate metabolism. We fused the N-terminal end of XylS to the maltose-binding protein (MBP) in vitro and found in glutaraldehyde cross-linking assays that the protein dimerized. Experiments with a chimeric N-terminal XylS linked to a ′LexA protein showed that the dimer was stabilized in the presence of alkylbenzoates. Sequence alignments with AraC and UreR allowed us to identify three residues, Leu193, Leu194, and Ile205, as potentially being involved in dimerization. Site-directed mutagenesis of XylS in which each of the above residues was replaced with Ala revealed that Leu193 and Leu194 were critical for activity and that a chimera in which LexA was linked to the N terminus of XylSLeu193Ala or XylSLeu194Ala was not functional. Dimerization of the chimeras MBP-N-XylSLeu193Ala and MBP-N-XylSLeu194Ala was not observed in cross-linking assays with glutaraldehyde. </jats:p> Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL <i>meta</i> -Cleavage Pathway, Are Involved in Dimerization Journal of Bacteriology |
| spellingShingle | Ruíz, Raquel, Marqués, Silvia, Ramos, Juan L., Journal of Bacteriology, Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization, Molecular Biology, Microbiology |
| title | Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_full | Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_fullStr | Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_full_unstemmed | Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_short | Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| title_sort | leucines 193 and 194 at the n-terminal domain of the xyls protein, the positive transcriptional regulator of the tol <i>meta</i> -cleavage pathway, are involved in dimerization |
| title_unstemmed | Leucines 193 and 194 at the N-Terminal Domain of the XylS Protein, the Positive Transcriptional Regulator of the TOL meta -Cleavage Pathway, Are Involved in Dimerization |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.185.10.3036-3041.2003 |