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Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Bacteriology, 191, 2009, 11, S. 3665-3676 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Krebs, Shelly J. Kirn, Thomas J. Taylor, Ronald K. Krebs, Shelly J. Kirn, Thomas J. Taylor, Ronald K. |
|---|---|
| author |
Krebs, Shelly J. Kirn, Thomas J. Taylor, Ronald K. |
| spellingShingle |
Krebs, Shelly J. Kirn, Thomas J. Taylor, Ronald K. Journal of Bacteriology Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor Molecular Biology Microbiology |
| author_sort |
krebs, shelly j. |
| spelling |
Krebs, Shelly J. Kirn, Thomas J. Taylor, Ronald K. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.01724-08 <jats:title>ABSTRACT</jats:title> <jats:p> Colonization of the human small intestine by <jats:italic>Vibrio cholerae</jats:italic> requires the type IV toxin-coregulated pilus (TCP). TcpF, which is encoded within the <jats:italic>tcp</jats:italic> operon, is secreted from the bacterial cell by the TCP apparatus and is also essential for colonization. Bacteria lacking <jats:italic>tcpF</jats:italic> are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. In order to elucidate the regions of the protein that are required for secretion through the TCP apparatus and for its function in colonization, random mutagenesis of <jats:italic>tcpF</jats:italic> was performed. Analysis of these mutants suggests that multiple regions throughout the protein influence extracellular secretion and that determinants near the C terminus are important for the function of TcpF in colonization. The TcpF proteins of certain environmental <jats:italic>V. cholerae</jats:italic> isolates with 31% to 66% identity to pathogenic <jats:italic>V. cholerae</jats:italic> TcpF showed higher similarity in regions identified as secretion determinants but diverged in regions found to be important for colonization. These environmental TcpF proteins are secreted from the pathogenic strain; however, they do not mediate colonization in the infant mouse model. Here we provide genetic evidence pointing toward regions of TcpF that influence secretion, as well as regions that play an important role in in vivo colonization. </jats:p> Genetic Mapping of Secretion and Functional Determinants of the <i>Vibrio cholerae</i> TcpF Colonization Factor Journal of Bacteriology |
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American Society for Microbiology, 2009 |
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American Society for Microbiology, 2009 |
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| title |
Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_unstemmed |
Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_full |
Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_fullStr |
Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_full_unstemmed |
Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_short |
Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_sort |
genetic mapping of secretion and functional determinants of the
<i>vibrio cholerae</i>
tcpf colonization factor |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.01724-08 |
| publishDate |
2009 |
| physical |
3665-3676 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Colonization of the human small intestine by
<jats:italic>Vibrio cholerae</jats:italic>
requires the type IV toxin-coregulated pilus (TCP). TcpF, which is encoded within the
<jats:italic>tcp</jats:italic>
operon, is secreted from the bacterial cell by the TCP apparatus and is also essential for colonization. Bacteria lacking
<jats:italic>tcpF</jats:italic>
are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. In order to elucidate the regions of the protein that are required for secretion through the TCP apparatus and for its function in colonization, random mutagenesis of
<jats:italic>tcpF</jats:italic>
was performed. Analysis of these mutants suggests that multiple regions throughout the protein influence extracellular secretion and that determinants near the C terminus are important for the function of TcpF in colonization. The TcpF proteins of certain environmental
<jats:italic>V. cholerae</jats:italic>
isolates with 31% to 66% identity to pathogenic
<jats:italic>V. cholerae</jats:italic>
TcpF showed higher similarity in regions identified as secretion determinants but diverged in regions found to be important for colonization. These environmental TcpF proteins are secreted from the pathogenic strain; however, they do not mediate colonization in the infant mouse model. Here we provide genetic evidence pointing toward regions of TcpF that influence secretion, as well as regions that play an important role in in vivo colonization.
</jats:p> |
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| author | Krebs, Shelly J., Kirn, Thomas J., Taylor, Ronald K. |
| author_facet | Krebs, Shelly J., Kirn, Thomas J., Taylor, Ronald K., Krebs, Shelly J., Kirn, Thomas J., Taylor, Ronald K. |
| author_sort | krebs, shelly j. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Colonization of the human small intestine by <jats:italic>Vibrio cholerae</jats:italic> requires the type IV toxin-coregulated pilus (TCP). TcpF, which is encoded within the <jats:italic>tcp</jats:italic> operon, is secreted from the bacterial cell by the TCP apparatus and is also essential for colonization. Bacteria lacking <jats:italic>tcpF</jats:italic> are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. In order to elucidate the regions of the protein that are required for secretion through the TCP apparatus and for its function in colonization, random mutagenesis of <jats:italic>tcpF</jats:italic> was performed. Analysis of these mutants suggests that multiple regions throughout the protein influence extracellular secretion and that determinants near the C terminus are important for the function of TcpF in colonization. The TcpF proteins of certain environmental <jats:italic>V. cholerae</jats:italic> isolates with 31% to 66% identity to pathogenic <jats:italic>V. cholerae</jats:italic> TcpF showed higher similarity in regions identified as secretion determinants but diverged in regions found to be important for colonization. These environmental TcpF proteins are secreted from the pathogenic strain; however, they do not mediate colonization in the infant mouse model. Here we provide genetic evidence pointing toward regions of TcpF that influence secretion, as well as regions that play an important role in in vivo colonization. </jats:p> |
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| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| physical | 3665-3676 |
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| spelling | Krebs, Shelly J. Kirn, Thomas J. Taylor, Ronald K. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.01724-08 <jats:title>ABSTRACT</jats:title> <jats:p> Colonization of the human small intestine by <jats:italic>Vibrio cholerae</jats:italic> requires the type IV toxin-coregulated pilus (TCP). TcpF, which is encoded within the <jats:italic>tcp</jats:italic> operon, is secreted from the bacterial cell by the TCP apparatus and is also essential for colonization. Bacteria lacking <jats:italic>tcpF</jats:italic> are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. In order to elucidate the regions of the protein that are required for secretion through the TCP apparatus and for its function in colonization, random mutagenesis of <jats:italic>tcpF</jats:italic> was performed. Analysis of these mutants suggests that multiple regions throughout the protein influence extracellular secretion and that determinants near the C terminus are important for the function of TcpF in colonization. The TcpF proteins of certain environmental <jats:italic>V. cholerae</jats:italic> isolates with 31% to 66% identity to pathogenic <jats:italic>V. cholerae</jats:italic> TcpF showed higher similarity in regions identified as secretion determinants but diverged in regions found to be important for colonization. These environmental TcpF proteins are secreted from the pathogenic strain; however, they do not mediate colonization in the infant mouse model. Here we provide genetic evidence pointing toward regions of TcpF that influence secretion, as well as regions that play an important role in in vivo colonization. </jats:p> Genetic Mapping of Secretion and Functional Determinants of the <i>Vibrio cholerae</i> TcpF Colonization Factor Journal of Bacteriology |
| spellingShingle | Krebs, Shelly J., Kirn, Thomas J., Taylor, Ronald K., Journal of Bacteriology, Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor, Molecular Biology, Microbiology |
| title | Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_full | Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_fullStr | Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_full_unstemmed | Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_short | Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| title_sort | genetic mapping of secretion and functional determinants of the <i>vibrio cholerae</i> tcpf colonization factor |
| title_unstemmed | Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.01724-08 |