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Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Journal of Clinical Microbiology, 41, 2003, 2, S. 712-716 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Dostál, Jií Hamal, Petr Pavlíčková, Libuše Souček, Milan Ruml, Tomáš Pichová, Iva Hrušková-Heidingsfeldová, Olga Dostál, Jií Hamal, Petr Pavlíčková, Libuše Souček, Milan Ruml, Tomáš Pichová, Iva Hrušková-Heidingsfeldová, Olga |
|---|---|
| author |
Dostál, Jií Hamal, Petr Pavlíčková, Libuše Souček, Milan Ruml, Tomáš Pichová, Iva Hrušková-Heidingsfeldová, Olga |
| spellingShingle |
Dostál, Jií Hamal, Petr Pavlíčková, Libuše Souček, Milan Ruml, Tomáš Pichová, Iva Hrušková-Heidingsfeldová, Olga Journal of Clinical Microbiology Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment Microbiology (medical) |
| author_sort |
dostál, jií |
| spelling |
Dostál, Jií Hamal, Petr Pavlíčková, Libuše Souček, Milan Ruml, Tomáš Pichová, Iva Hrušková-Heidingsfeldová, Olga 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.41.2.712-716.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The yeasts of the genus <jats:italic>Candida</jats:italic> are opportunistic pathogens associated with the rising incidence of life-threatening infections in immunocompromised individuals. Secretion of aspartic proteinases has been determined to be one of the virulence factors of the pathogenic <jats:italic>Candida</jats:italic> species. To analyze the extracellular proteolytic activities of a large number of <jats:italic>Candida</jats:italic> clinical isolates, we developed a screening system based on a solid medium containing hemoglobin as the sole nitrogen source. The cleavage of hemoglobin by the secreted proteinases results in formation of clearance zones. The visibility of such zones was enhanced by addition of an acid-base indicator. Using this system, we assessed 245 clinical isolates of <jats:italic>Candida</jats:italic> from patients in the hospital of the Faculty of Medicine, Palacky University, Olomouc, Czech Republic, for the presence of secreted aspartic proteases (Saps). We also used the test plates for rapid semiquantitative testing of Sap inhibitors. Most of the pepstatin analogs affected the formation of the zones of clearance as well as the growth of <jats:italic>Candida albicans</jats:italic> , <jats:italic>C. tropicalis</jats:italic> , and <jats:italic>C. parapsilosis</jats:italic> colonies. By contrast, the human immunodeficiency virus proteinase inhibitors saquinavir, ritonavir, nelfinavir, and indinavir had no effect on the <jats:italic>Candida</jats:italic> strains tested. These results are in agreement with the inhibition constants obtained for the individual inhibitors with purified Saps. Thus, the plates containing hemoglobin proved to be an appropriate tool for the rapid and reliable assessment of Sap production and inhibition. </jats:p> Simple Method for Screening <i>Candida</i> Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment Journal of Clinical Microbiology |
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| title |
Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_unstemmed |
Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_full |
Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_fullStr |
Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_full_unstemmed |
Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_short |
Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_sort |
simple method for screening
<i>candida</i>
species isolates for the presence of secreted proteinases: a tool for the prediction of successful inhibitory treatment |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.41.2.712-716.2003 |
| publishDate |
2003 |
| physical |
712-716 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The yeasts of the genus
<jats:italic>Candida</jats:italic>
are opportunistic pathogens associated with the rising incidence of life-threatening infections in immunocompromised individuals. Secretion of aspartic proteinases has been determined to be one of the virulence factors of the pathogenic
<jats:italic>Candida</jats:italic>
species. To analyze the extracellular proteolytic activities of a large number of
<jats:italic>Candida</jats:italic>
clinical isolates, we developed a screening system based on a solid medium containing hemoglobin as the sole nitrogen source. The cleavage of hemoglobin by the secreted proteinases results in formation of clearance zones. The visibility of such zones was enhanced by addition of an acid-base indicator. Using this system, we assessed 245 clinical isolates of
<jats:italic>Candida</jats:italic>
from patients in the hospital of the Faculty of Medicine, Palacky University, Olomouc, Czech Republic, for the presence of secreted aspartic proteases (Saps). We also used the test plates for rapid semiquantitative testing of Sap inhibitors. Most of the pepstatin analogs affected the formation of the zones of clearance as well as the growth of
<jats:italic>Candida albicans</jats:italic>
,
<jats:italic>C. tropicalis</jats:italic>
, and
<jats:italic>C. parapsilosis</jats:italic>
colonies. By contrast, the human immunodeficiency virus proteinase inhibitors saquinavir, ritonavir, nelfinavir, and indinavir had no effect on the
<jats:italic>Candida</jats:italic>
strains tested. These results are in agreement with the inhibition constants obtained for the individual inhibitors with purified Saps. Thus, the plates containing hemoglobin proved to be an appropriate tool for the rapid and reliable assessment of Sap production and inhibition.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The yeasts of the genus <jats:italic>Candida</jats:italic> are opportunistic pathogens associated with the rising incidence of life-threatening infections in immunocompromised individuals. Secretion of aspartic proteinases has been determined to be one of the virulence factors of the pathogenic <jats:italic>Candida</jats:italic> species. To analyze the extracellular proteolytic activities of a large number of <jats:italic>Candida</jats:italic> clinical isolates, we developed a screening system based on a solid medium containing hemoglobin as the sole nitrogen source. The cleavage of hemoglobin by the secreted proteinases results in formation of clearance zones. The visibility of such zones was enhanced by addition of an acid-base indicator. Using this system, we assessed 245 clinical isolates of <jats:italic>Candida</jats:italic> from patients in the hospital of the Faculty of Medicine, Palacky University, Olomouc, Czech Republic, for the presence of secreted aspartic proteases (Saps). We also used the test plates for rapid semiquantitative testing of Sap inhibitors. Most of the pepstatin analogs affected the formation of the zones of clearance as well as the growth of <jats:italic>Candida albicans</jats:italic> , <jats:italic>C. tropicalis</jats:italic> , and <jats:italic>C. parapsilosis</jats:italic> colonies. By contrast, the human immunodeficiency virus proteinase inhibitors saquinavir, ritonavir, nelfinavir, and indinavir had no effect on the <jats:italic>Candida</jats:italic> strains tested. These results are in agreement with the inhibition constants obtained for the individual inhibitors with purified Saps. Thus, the plates containing hemoglobin proved to be an appropriate tool for the rapid and reliable assessment of Sap production and inhibition. </jats:p> |
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| spelling | Dostál, Jií Hamal, Petr Pavlíčková, Libuše Souček, Milan Ruml, Tomáš Pichová, Iva Hrušková-Heidingsfeldová, Olga 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.41.2.712-716.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The yeasts of the genus <jats:italic>Candida</jats:italic> are opportunistic pathogens associated with the rising incidence of life-threatening infections in immunocompromised individuals. Secretion of aspartic proteinases has been determined to be one of the virulence factors of the pathogenic <jats:italic>Candida</jats:italic> species. To analyze the extracellular proteolytic activities of a large number of <jats:italic>Candida</jats:italic> clinical isolates, we developed a screening system based on a solid medium containing hemoglobin as the sole nitrogen source. The cleavage of hemoglobin by the secreted proteinases results in formation of clearance zones. The visibility of such zones was enhanced by addition of an acid-base indicator. Using this system, we assessed 245 clinical isolates of <jats:italic>Candida</jats:italic> from patients in the hospital of the Faculty of Medicine, Palacky University, Olomouc, Czech Republic, for the presence of secreted aspartic proteases (Saps). We also used the test plates for rapid semiquantitative testing of Sap inhibitors. Most of the pepstatin analogs affected the formation of the zones of clearance as well as the growth of <jats:italic>Candida albicans</jats:italic> , <jats:italic>C. tropicalis</jats:italic> , and <jats:italic>C. parapsilosis</jats:italic> colonies. By contrast, the human immunodeficiency virus proteinase inhibitors saquinavir, ritonavir, nelfinavir, and indinavir had no effect on the <jats:italic>Candida</jats:italic> strains tested. These results are in agreement with the inhibition constants obtained for the individual inhibitors with purified Saps. Thus, the plates containing hemoglobin proved to be an appropriate tool for the rapid and reliable assessment of Sap production and inhibition. </jats:p> Simple Method for Screening <i>Candida</i> Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment Journal of Clinical Microbiology |
| spellingShingle | Dostál, Jií, Hamal, Petr, Pavlíčková, Libuše, Souček, Milan, Ruml, Tomáš, Pichová, Iva, Hrušková-Heidingsfeldová, Olga, Journal of Clinical Microbiology, Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment, Microbiology (medical) |
| title | Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_full | Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_fullStr | Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_full_unstemmed | Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_short | Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| title_sort | simple method for screening <i>candida</i> species isolates for the presence of secreted proteinases: a tool for the prediction of successful inhibitory treatment |
| title_unstemmed | Simple Method for Screening Candida Species Isolates for the Presence of Secreted Proteinases: a Tool for the Prediction of Successful Inhibitory Treatment |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.41.2.712-716.2003 |