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Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Clinical Microbiology, 38, 2000, 11, S. 3971-3978 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Wilson, David L. Abner, Sheila R. Newman, Thomas C. Mansfield, Linda S. Linz, John E. Wilson, David L. Abner, Sheila R. Newman, Thomas C. Mansfield, Linda S. Linz, John E. |
|---|---|
| author |
Wilson, David L. Abner, Sheila R. Newman, Thomas C. Mansfield, Linda S. Linz, John E. |
| spellingShingle |
Wilson, David L. Abner, Sheila R. Newman, Thomas C. Mansfield, Linda S. Linz, John E. Journal of Clinical Microbiology Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay Microbiology (medical) |
| author_sort |
wilson, david l. |
| spelling |
Wilson, David L. Abner, Sheila R. Newman, Thomas C. Mansfield, Linda S. Linz, John E. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.38.11.3971-3978.2000 <jats:title>ABSTRACT</jats:title> <jats:p> Fluoroquinolones are one class of antimicrobial agents commonly used to treat severe <jats:italic>Campylobacter jejuni</jats:italic> infection. <jats:italic>C. jejuni</jats:italic> strains resistant to high levels of the fluoroquinolone ciprofloxacin (MIC ≥16 μg/ml) have been predominantly characterized with a C→T transition in codon 86 of <jats:italic>gyrA</jats:italic> . The <jats:italic>gyrA</jats:italic> gene encodes one subunit of DNA gyrase, which is a primary target for fluoroquinolone antibiotics. This study establishes a rapid PCR-based TaqMan method for identifying ciprofloxacin-resistant <jats:italic>C. jejuni</jats:italic> strains that carry the C→T transition in codon 86 of <jats:italic>gyrA</jats:italic> . The assay uses real-time detection, eliminating the need for gel electrophoresis. Optimization of the assay parameters using purified <jats:italic>Campylobacter</jats:italic> DNA resulted in the ability to detect femtogram levels of DNA. The method should be useful for monitoring the development of ciprofloxacin resistance in <jats:italic>C. jejuni</jats:italic> . Compiled nucleotide sequence data on the quinolone resistance-determining region of <jats:italic>gyrA</jats:italic> in <jats:italic>Campylobacter</jats:italic> indicate that sequence comparison of this region is a useful method for tentative identification of <jats:italic>Campylobacter</jats:italic> isolates at the species level. </jats:p> Identification of Ciprofloxacin-Resistant <i>Campylobacter jejuni</i> by Use of a Fluorogenic PCR Assay Journal of Clinical Microbiology |
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| title |
Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_unstemmed |
Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_full |
Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_fullStr |
Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_full_unstemmed |
Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_short |
Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_sort |
identification of ciprofloxacin-resistant
<i>campylobacter jejuni</i>
by use of a fluorogenic pcr assay |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.38.11.3971-3978.2000 |
| publishDate |
2000 |
| physical |
3971-3978 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Fluoroquinolones are one class of antimicrobial agents commonly used to treat severe
<jats:italic>Campylobacter jejuni</jats:italic>
infection.
<jats:italic>C. jejuni</jats:italic>
strains resistant to high levels of the fluoroquinolone ciprofloxacin (MIC ≥16 μg/ml) have been predominantly characterized with a C→T transition in codon 86 of
<jats:italic>gyrA</jats:italic>
. The
<jats:italic>gyrA</jats:italic>
gene encodes one subunit of DNA gyrase, which is a primary target for fluoroquinolone antibiotics. This study establishes a rapid PCR-based TaqMan method for identifying ciprofloxacin-resistant
<jats:italic>C. jejuni</jats:italic>
strains that carry the C→T transition in codon 86 of
<jats:italic>gyrA</jats:italic>
. The assay uses real-time detection, eliminating the need for gel electrophoresis. Optimization of the assay parameters using purified
<jats:italic>Campylobacter</jats:italic>
DNA resulted in the ability to detect femtogram levels of DNA. The method should be useful for monitoring the development of ciprofloxacin resistance in
<jats:italic>C. jejuni</jats:italic>
. Compiled nucleotide sequence data on the quinolone resistance-determining region of
<jats:italic>gyrA</jats:italic>
in
<jats:italic>Campylobacter</jats:italic>
indicate that sequence comparison of this region is a useful method for tentative identification of
<jats:italic>Campylobacter</jats:italic>
isolates at the species level.
</jats:p> |
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| author | Wilson, David L., Abner, Sheila R., Newman, Thomas C., Mansfield, Linda S., Linz, John E. |
| author_facet | Wilson, David L., Abner, Sheila R., Newman, Thomas C., Mansfield, Linda S., Linz, John E., Wilson, David L., Abner, Sheila R., Newman, Thomas C., Mansfield, Linda S., Linz, John E. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Fluoroquinolones are one class of antimicrobial agents commonly used to treat severe <jats:italic>Campylobacter jejuni</jats:italic> infection. <jats:italic>C. jejuni</jats:italic> strains resistant to high levels of the fluoroquinolone ciprofloxacin (MIC ≥16 μg/ml) have been predominantly characterized with a C→T transition in codon 86 of <jats:italic>gyrA</jats:italic> . The <jats:italic>gyrA</jats:italic> gene encodes one subunit of DNA gyrase, which is a primary target for fluoroquinolone antibiotics. This study establishes a rapid PCR-based TaqMan method for identifying ciprofloxacin-resistant <jats:italic>C. jejuni</jats:italic> strains that carry the C→T transition in codon 86 of <jats:italic>gyrA</jats:italic> . The assay uses real-time detection, eliminating the need for gel electrophoresis. Optimization of the assay parameters using purified <jats:italic>Campylobacter</jats:italic> DNA resulted in the ability to detect femtogram levels of DNA. The method should be useful for monitoring the development of ciprofloxacin resistance in <jats:italic>C. jejuni</jats:italic> . Compiled nucleotide sequence data on the quinolone resistance-determining region of <jats:italic>gyrA</jats:italic> in <jats:italic>Campylobacter</jats:italic> indicate that sequence comparison of this region is a useful method for tentative identification of <jats:italic>Campylobacter</jats:italic> isolates at the species level. </jats:p> |
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| spelling | Wilson, David L. Abner, Sheila R. Newman, Thomas C. Mansfield, Linda S. Linz, John E. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.38.11.3971-3978.2000 <jats:title>ABSTRACT</jats:title> <jats:p> Fluoroquinolones are one class of antimicrobial agents commonly used to treat severe <jats:italic>Campylobacter jejuni</jats:italic> infection. <jats:italic>C. jejuni</jats:italic> strains resistant to high levels of the fluoroquinolone ciprofloxacin (MIC ≥16 μg/ml) have been predominantly characterized with a C→T transition in codon 86 of <jats:italic>gyrA</jats:italic> . The <jats:italic>gyrA</jats:italic> gene encodes one subunit of DNA gyrase, which is a primary target for fluoroquinolone antibiotics. This study establishes a rapid PCR-based TaqMan method for identifying ciprofloxacin-resistant <jats:italic>C. jejuni</jats:italic> strains that carry the C→T transition in codon 86 of <jats:italic>gyrA</jats:italic> . The assay uses real-time detection, eliminating the need for gel electrophoresis. Optimization of the assay parameters using purified <jats:italic>Campylobacter</jats:italic> DNA resulted in the ability to detect femtogram levels of DNA. The method should be useful for monitoring the development of ciprofloxacin resistance in <jats:italic>C. jejuni</jats:italic> . Compiled nucleotide sequence data on the quinolone resistance-determining region of <jats:italic>gyrA</jats:italic> in <jats:italic>Campylobacter</jats:italic> indicate that sequence comparison of this region is a useful method for tentative identification of <jats:italic>Campylobacter</jats:italic> isolates at the species level. </jats:p> Identification of Ciprofloxacin-Resistant <i>Campylobacter jejuni</i> by Use of a Fluorogenic PCR Assay Journal of Clinical Microbiology |
| spellingShingle | Wilson, David L., Abner, Sheila R., Newman, Thomas C., Mansfield, Linda S., Linz, John E., Journal of Clinical Microbiology, Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay, Microbiology (medical) |
| title | Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_full | Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_fullStr | Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_full_unstemmed | Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_short | Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| title_sort | identification of ciprofloxacin-resistant <i>campylobacter jejuni</i> by use of a fluorogenic pcr assay |
| title_unstemmed | Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.38.11.3971-3978.2000 |