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Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Ch...
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Zeitschriftentitel: | Journal of Clinical Microbiology |
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Personen und Körperschaften: | , , , , , , , , , , |
In: | Journal of Clinical Microbiology, 38, 2000, 11, S. 3953-3959 |
Format: | E-Article |
Sprache: | Englisch |
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American Society for Microbiology
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author_facet |
Goh, Swee Han Facklam, Richard R. Chang, Michelle Hill, Janet E. Tyrrell, Gregory J. Burns, Emma C. M. Chan, David He, Cheng Rahim, Tazim Shaw, Carol Hemmingsen, Sean M. Goh, Swee Han Facklam, Richard R. Chang, Michelle Hill, Janet E. Tyrrell, Gregory J. Burns, Emma C. M. Chan, David He, Cheng Rahim, Tazim Shaw, Carol Hemmingsen, Sean M. |
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author |
Goh, Swee Han Facklam, Richard R. Chang, Michelle Hill, Janet E. Tyrrell, Gregory J. Burns, Emma C. M. Chan, David He, Cheng Rahim, Tazim Shaw, Carol Hemmingsen, Sean M. |
spellingShingle |
Goh, Swee Han Facklam, Richard R. Chang, Michelle Hill, Janet E. Tyrrell, Gregory J. Burns, Emma C. M. Chan, David He, Cheng Rahim, Tazim Shaw, Carol Hemmingsen, Sean M. Journal of Clinical Microbiology Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences Microbiology (medical) |
author_sort |
goh, swee han |
spelling |
Goh, Swee Han Facklam, Richard R. Chang, Michelle Hill, Janet E. Tyrrell, Gregory J. Burns, Emma C. M. Chan, David He, Cheng Rahim, Tazim Shaw, Carol Hemmingsen, Sean M. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.38.11.3953-3959.2000 <jats:title>ABSTRACT</jats:title> <jats:p> Data from four recent studies (S. H. Goh et al., J. Clin. Microbiol. 36:2164–2166, 1998; S. H. Goh et al., J. Clin. Microbiol. 34:818–823, 1996; S. H. Goh et al., J. Clin. Microbiol. 35:3116–3121, 1997; A. Y. C. Kwok et al., Int. J. Syst. Bacteriol. 49:1181–1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (Cpn60) gene, amplified by PCR with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (ID). This Cpn60 gene ID method correctly identified isolates representative of numerous staphylococcal species and <jats:italic>Streptococcus iniae</jats:italic> , a human and animal pathogen. We report herein that this method enabled us to distinguish clearly between 17 <jats:italic>Enterococcus</jats:italic> species ( <jats:italic>Enterococcus asini</jats:italic> , <jats:italic>Enterococcus rattus</jats:italic> , <jats:italic>Enterococcus dispar</jats:italic> , <jats:italic>Enterococcus gallinarum</jats:italic> , <jats:italic>Enterococcus hirae</jats:italic> , <jats:italic>Enterococcus durans</jats:italic> , <jats:italic>Enterococcus cecorum</jats:italic> , <jats:italic>Enterococcus faecalis</jats:italic> , <jats:italic>Enterococcus mundtii</jats:italic> , <jats:italic>Enterococcus casseliflavus</jats:italic> , <jats:italic>Enterococcus faecium</jats:italic> , <jats:italic>Enterococcus malodoratus</jats:italic> , <jats:italic>Enterococcus raffinosus</jats:italic> , <jats:italic>Enterococcus avium</jats:italic> , <jats:italic>Enterococcus pseudoavium</jats:italic> , <jats:italic>Enterococcus</jats:italic> new sp. strain Facklam, and <jats:italic>Enterococcus saccharolyticus</jats:italic> ), and <jats:italic>Vagococcus fluvialis</jats:italic> , <jats:italic>Lactococcus lactis</jats:italic> , and <jats:italic>Lactococcus garvieae</jats:italic> . From 123 blind-tested samples, only two discrepancies were observed between the Facklam and Collins phenotyping method (R. R. Facklam and M. D. Collins, J. Clin. Microbiol. 27:731–734, 1989) and the Cpn60 ID method. In each case, the discrepancies were resolved in favor of the Cpn60 ID method. The species distributions of the 123 blind-tested isolates were <jats:italic>Enterococcus</jats:italic> new sp. strain Facklam (ATCC 700913), 3; <jats:italic>E. asini</jats:italic> , 1; <jats:italic>E. rattus</jats:italic> , 4; <jats:italic>E. dispar</jats:italic> , 2; <jats:italic>E. gallinarum</jats:italic> , 20; <jats:italic>E. hirae</jats:italic> , 9; <jats:italic>E. durans</jats:italic> , 9; <jats:italic>E. faecalis</jats:italic> , 12; <jats:italic>E. mundtii</jats:italic> , 3; <jats:italic>E. casseliflavus</jats:italic> , 8; <jats:italic>E. faecium</jats:italic> , 25; <jats:italic>E. malodoratus</jats:italic> , 3; <jats:italic>E. raffinosus</jats:italic> , 8; <jats:italic>E. avium</jats:italic> , 4; <jats:italic>E. pseudoavium</jats:italic> , 1; an unknown <jats:italic>Enterococcus</jats:italic> clinical isolate, sp. strain R871; <jats:italic>Vagococcus fluvialis</jats:italic> , 4; <jats:italic>Lactococcus garvieae</jats:italic> , 3; <jats:italic>Lactococcus lactis</jats:italic> , 3; <jats:italic>Leuconostoc</jats:italic> sp., 1; and <jats:italic>Pediococcus</jats:italic> sp., 1. The Cpn60 gene ID method, coupled with reverse checkerboard hybridization, is an effective method for the identification of <jats:italic>Enterococcus</jats:italic> and related organisms. </jats:p> Identification of <i>Enterococcus</i> Species and Phenotypically Similar <i>Lactococcus</i> and <i>Vagococcus</i> Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences Journal of Clinical Microbiology |
doi_str_mv |
10.1128/jcm.38.11.3953-3959.2000 |
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Online Free |
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ElectronicArticle |
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American Society for Microbiology, 2000 |
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American Society for Microbiology, 2000 |
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0095-1137 1098-660X |
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0095-1137 1098-660X |
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goh2000identificationofenterococcusspeciesandphenotypicallysimilarlactococcusandvagococcusspeciesbyreversecheckerboardhybridizationtochaperonin60genesequences |
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2000 |
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American Society for Microbiology |
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Journal of Clinical Microbiology |
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title |
Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_unstemmed |
Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_full |
Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_fullStr |
Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_full_unstemmed |
Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_short |
Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_sort |
identification of
<i>enterococcus</i>
species and phenotypically similar
<i>lactococcus</i>
and
<i>vagococcus</i>
species by reverse checkerboard hybridization to chaperonin 60 gene sequences |
topic |
Microbiology (medical) |
url |
http://dx.doi.org/10.1128/jcm.38.11.3953-3959.2000 |
publishDate |
2000 |
physical |
3953-3959 |
description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Data from four recent studies (S. H. Goh et al., J. Clin. Microbiol. 36:2164–2166, 1998; S. H. Goh et al., J. Clin. Microbiol. 34:818–823, 1996; S. H. Goh et al., J. Clin. Microbiol. 35:3116–3121, 1997; A. Y. C. Kwok et al., Int. J. Syst. Bacteriol. 49:1181–1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (Cpn60) gene, amplified by PCR with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (ID). This Cpn60 gene ID method correctly identified isolates representative of numerous staphylococcal species and
<jats:italic>Streptococcus iniae</jats:italic>
, a human and animal pathogen. We report herein that this method enabled us to distinguish clearly between 17
<jats:italic>Enterococcus</jats:italic>
species (
<jats:italic>Enterococcus asini</jats:italic>
,
<jats:italic>Enterococcus rattus</jats:italic>
,
<jats:italic>Enterococcus dispar</jats:italic>
,
<jats:italic>Enterococcus gallinarum</jats:italic>
,
<jats:italic>Enterococcus hirae</jats:italic>
,
<jats:italic>Enterococcus durans</jats:italic>
,
<jats:italic>Enterococcus cecorum</jats:italic>
,
<jats:italic>Enterococcus faecalis</jats:italic>
,
<jats:italic>Enterococcus mundtii</jats:italic>
,
<jats:italic>Enterococcus casseliflavus</jats:italic>
,
<jats:italic>Enterococcus faecium</jats:italic>
,
<jats:italic>Enterococcus malodoratus</jats:italic>
,
<jats:italic>Enterococcus raffinosus</jats:italic>
,
<jats:italic>Enterococcus avium</jats:italic>
,
<jats:italic>Enterococcus pseudoavium</jats:italic>
,
<jats:italic>Enterococcus</jats:italic>
new sp. strain Facklam, and
<jats:italic>Enterococcus saccharolyticus</jats:italic>
), and
<jats:italic>Vagococcus fluvialis</jats:italic>
,
<jats:italic>Lactococcus lactis</jats:italic>
, and
<jats:italic>Lactococcus garvieae</jats:italic>
. From 123 blind-tested samples, only two discrepancies were observed between the Facklam and Collins phenotyping method (R. R. Facklam and M. D. Collins, J. Clin. Microbiol. 27:731–734, 1989) and the Cpn60 ID method. In each case, the discrepancies were resolved in favor of the Cpn60 ID method. The species distributions of the 123 blind-tested isolates were
<jats:italic>Enterococcus</jats:italic>
new sp. strain Facklam (ATCC 700913), 3;
<jats:italic>E. asini</jats:italic>
, 1;
<jats:italic>E. rattus</jats:italic>
, 4;
<jats:italic>E. dispar</jats:italic>
, 2;
<jats:italic>E. gallinarum</jats:italic>
, 20;
<jats:italic>E. hirae</jats:italic>
, 9;
<jats:italic>E. durans</jats:italic>
, 9;
<jats:italic>E. faecalis</jats:italic>
, 12;
<jats:italic>E. mundtii</jats:italic>
, 3;
<jats:italic>E. casseliflavus</jats:italic>
, 8;
<jats:italic>E. faecium</jats:italic>
, 25;
<jats:italic>E. malodoratus</jats:italic>
, 3;
<jats:italic>E. raffinosus</jats:italic>
, 8;
<jats:italic>E. avium</jats:italic>
, 4;
<jats:italic>E. pseudoavium</jats:italic>
, 1; an unknown
<jats:italic>Enterococcus</jats:italic>
clinical isolate, sp. strain R871;
<jats:italic>Vagococcus fluvialis</jats:italic>
, 4;
<jats:italic>Lactococcus garvieae</jats:italic>
, 3;
<jats:italic>Lactococcus lactis</jats:italic>
, 3;
<jats:italic>Leuconostoc</jats:italic>
sp., 1; and
<jats:italic>Pediococcus</jats:italic>
sp., 1. The Cpn60 gene ID method, coupled with reverse checkerboard hybridization, is an effective method for the identification of
<jats:italic>Enterococcus</jats:italic>
and related organisms.
</jats:p> |
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author | Goh, Swee Han, Facklam, Richard R., Chang, Michelle, Hill, Janet E., Tyrrell, Gregory J., Burns, Emma C. M., Chan, David, He, Cheng, Rahim, Tazim, Shaw, Carol, Hemmingsen, Sean M. |
author_facet | Goh, Swee Han, Facklam, Richard R., Chang, Michelle, Hill, Janet E., Tyrrell, Gregory J., Burns, Emma C. M., Chan, David, He, Cheng, Rahim, Tazim, Shaw, Carol, Hemmingsen, Sean M., Goh, Swee Han, Facklam, Richard R., Chang, Michelle, Hill, Janet E., Tyrrell, Gregory J., Burns, Emma C. M., Chan, David, He, Cheng, Rahim, Tazim, Shaw, Carol, Hemmingsen, Sean M. |
author_sort | goh, swee han |
container_issue | 11 |
container_start_page | 3953 |
container_title | Journal of Clinical Microbiology |
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description | <jats:title>ABSTRACT</jats:title> <jats:p> Data from four recent studies (S. H. Goh et al., J. Clin. Microbiol. 36:2164–2166, 1998; S. H. Goh et al., J. Clin. Microbiol. 34:818–823, 1996; S. H. Goh et al., J. Clin. Microbiol. 35:3116–3121, 1997; A. Y. C. Kwok et al., Int. J. Syst. Bacteriol. 49:1181–1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (Cpn60) gene, amplified by PCR with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (ID). This Cpn60 gene ID method correctly identified isolates representative of numerous staphylococcal species and <jats:italic>Streptococcus iniae</jats:italic> , a human and animal pathogen. We report herein that this method enabled us to distinguish clearly between 17 <jats:italic>Enterococcus</jats:italic> species ( <jats:italic>Enterococcus asini</jats:italic> , <jats:italic>Enterococcus rattus</jats:italic> , <jats:italic>Enterococcus dispar</jats:italic> , <jats:italic>Enterococcus gallinarum</jats:italic> , <jats:italic>Enterococcus hirae</jats:italic> , <jats:italic>Enterococcus durans</jats:italic> , <jats:italic>Enterococcus cecorum</jats:italic> , <jats:italic>Enterococcus faecalis</jats:italic> , <jats:italic>Enterococcus mundtii</jats:italic> , <jats:italic>Enterococcus casseliflavus</jats:italic> , <jats:italic>Enterococcus faecium</jats:italic> , <jats:italic>Enterococcus malodoratus</jats:italic> , <jats:italic>Enterococcus raffinosus</jats:italic> , <jats:italic>Enterococcus avium</jats:italic> , <jats:italic>Enterococcus pseudoavium</jats:italic> , <jats:italic>Enterococcus</jats:italic> new sp. strain Facklam, and <jats:italic>Enterococcus saccharolyticus</jats:italic> ), and <jats:italic>Vagococcus fluvialis</jats:italic> , <jats:italic>Lactococcus lactis</jats:italic> , and <jats:italic>Lactococcus garvieae</jats:italic> . From 123 blind-tested samples, only two discrepancies were observed between the Facklam and Collins phenotyping method (R. R. Facklam and M. D. Collins, J. Clin. Microbiol. 27:731–734, 1989) and the Cpn60 ID method. In each case, the discrepancies were resolved in favor of the Cpn60 ID method. The species distributions of the 123 blind-tested isolates were <jats:italic>Enterococcus</jats:italic> new sp. strain Facklam (ATCC 700913), 3; <jats:italic>E. asini</jats:italic> , 1; <jats:italic>E. rattus</jats:italic> , 4; <jats:italic>E. dispar</jats:italic> , 2; <jats:italic>E. gallinarum</jats:italic> , 20; <jats:italic>E. hirae</jats:italic> , 9; <jats:italic>E. durans</jats:italic> , 9; <jats:italic>E. faecalis</jats:italic> , 12; <jats:italic>E. mundtii</jats:italic> , 3; <jats:italic>E. casseliflavus</jats:italic> , 8; <jats:italic>E. faecium</jats:italic> , 25; <jats:italic>E. malodoratus</jats:italic> , 3; <jats:italic>E. raffinosus</jats:italic> , 8; <jats:italic>E. avium</jats:italic> , 4; <jats:italic>E. pseudoavium</jats:italic> , 1; an unknown <jats:italic>Enterococcus</jats:italic> clinical isolate, sp. strain R871; <jats:italic>Vagococcus fluvialis</jats:italic> , 4; <jats:italic>Lactococcus garvieae</jats:italic> , 3; <jats:italic>Lactococcus lactis</jats:italic> , 3; <jats:italic>Leuconostoc</jats:italic> sp., 1; and <jats:italic>Pediococcus</jats:italic> sp., 1. The Cpn60 gene ID method, coupled with reverse checkerboard hybridization, is an effective method for the identification of <jats:italic>Enterococcus</jats:italic> and related organisms. </jats:p> |
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series | Journal of Clinical Microbiology |
source_id | 49 |
spelling | Goh, Swee Han Facklam, Richard R. Chang, Michelle Hill, Janet E. Tyrrell, Gregory J. Burns, Emma C. M. Chan, David He, Cheng Rahim, Tazim Shaw, Carol Hemmingsen, Sean M. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.38.11.3953-3959.2000 <jats:title>ABSTRACT</jats:title> <jats:p> Data from four recent studies (S. H. Goh et al., J. Clin. Microbiol. 36:2164–2166, 1998; S. H. Goh et al., J. Clin. Microbiol. 34:818–823, 1996; S. H. Goh et al., J. Clin. Microbiol. 35:3116–3121, 1997; A. Y. C. Kwok et al., Int. J. Syst. Bacteriol. 49:1181–1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (Cpn60) gene, amplified by PCR with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (ID). This Cpn60 gene ID method correctly identified isolates representative of numerous staphylococcal species and <jats:italic>Streptococcus iniae</jats:italic> , a human and animal pathogen. We report herein that this method enabled us to distinguish clearly between 17 <jats:italic>Enterococcus</jats:italic> species ( <jats:italic>Enterococcus asini</jats:italic> , <jats:italic>Enterococcus rattus</jats:italic> , <jats:italic>Enterococcus dispar</jats:italic> , <jats:italic>Enterococcus gallinarum</jats:italic> , <jats:italic>Enterococcus hirae</jats:italic> , <jats:italic>Enterococcus durans</jats:italic> , <jats:italic>Enterococcus cecorum</jats:italic> , <jats:italic>Enterococcus faecalis</jats:italic> , <jats:italic>Enterococcus mundtii</jats:italic> , <jats:italic>Enterococcus casseliflavus</jats:italic> , <jats:italic>Enterococcus faecium</jats:italic> , <jats:italic>Enterococcus malodoratus</jats:italic> , <jats:italic>Enterococcus raffinosus</jats:italic> , <jats:italic>Enterococcus avium</jats:italic> , <jats:italic>Enterococcus pseudoavium</jats:italic> , <jats:italic>Enterococcus</jats:italic> new sp. strain Facklam, and <jats:italic>Enterococcus saccharolyticus</jats:italic> ), and <jats:italic>Vagococcus fluvialis</jats:italic> , <jats:italic>Lactococcus lactis</jats:italic> , and <jats:italic>Lactococcus garvieae</jats:italic> . From 123 blind-tested samples, only two discrepancies were observed between the Facklam and Collins phenotyping method (R. R. Facklam and M. D. Collins, J. Clin. Microbiol. 27:731–734, 1989) and the Cpn60 ID method. In each case, the discrepancies were resolved in favor of the Cpn60 ID method. The species distributions of the 123 blind-tested isolates were <jats:italic>Enterococcus</jats:italic> new sp. strain Facklam (ATCC 700913), 3; <jats:italic>E. asini</jats:italic> , 1; <jats:italic>E. rattus</jats:italic> , 4; <jats:italic>E. dispar</jats:italic> , 2; <jats:italic>E. gallinarum</jats:italic> , 20; <jats:italic>E. hirae</jats:italic> , 9; <jats:italic>E. durans</jats:italic> , 9; <jats:italic>E. faecalis</jats:italic> , 12; <jats:italic>E. mundtii</jats:italic> , 3; <jats:italic>E. casseliflavus</jats:italic> , 8; <jats:italic>E. faecium</jats:italic> , 25; <jats:italic>E. malodoratus</jats:italic> , 3; <jats:italic>E. raffinosus</jats:italic> , 8; <jats:italic>E. avium</jats:italic> , 4; <jats:italic>E. pseudoavium</jats:italic> , 1; an unknown <jats:italic>Enterococcus</jats:italic> clinical isolate, sp. strain R871; <jats:italic>Vagococcus fluvialis</jats:italic> , 4; <jats:italic>Lactococcus garvieae</jats:italic> , 3; <jats:italic>Lactococcus lactis</jats:italic> , 3; <jats:italic>Leuconostoc</jats:italic> sp., 1; and <jats:italic>Pediococcus</jats:italic> sp., 1. The Cpn60 gene ID method, coupled with reverse checkerboard hybridization, is an effective method for the identification of <jats:italic>Enterococcus</jats:italic> and related organisms. </jats:p> Identification of <i>Enterococcus</i> Species and Phenotypically Similar <i>Lactococcus</i> and <i>Vagococcus</i> Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences Journal of Clinical Microbiology |
spellingShingle | Goh, Swee Han, Facklam, Richard R., Chang, Michelle, Hill, Janet E., Tyrrell, Gregory J., Burns, Emma C. M., Chan, David, He, Cheng, Rahim, Tazim, Shaw, Carol, Hemmingsen, Sean M., Journal of Clinical Microbiology, Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences, Microbiology (medical) |
title | Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_full | Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_fullStr | Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_full_unstemmed | Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_short | Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
title_sort | identification of <i>enterococcus</i> species and phenotypically similar <i>lactococcus</i> and <i>vagococcus</i> species by reverse checkerboard hybridization to chaperonin 60 gene sequences |
title_unstemmed | Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences |
topic | Microbiology (medical) |
url | http://dx.doi.org/10.1128/jcm.38.11.3953-3959.2000 |