Eintrag weiter verarbeiten
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa
Gespeichert in:
| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Journal of Clinical Microbiology, 29, 1991, 10, S. 2280-2284 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Behets, F Disasi, A Ryder, R W Bishagara, K Piot, P Kashamuka, M Kamenga, M Nzila, N Laga, M Vercauteren, G Behets, F Disasi, A Ryder, R W Bishagara, K Piot, P Kashamuka, M Kamenga, M Nzila, N Laga, M Vercauteren, G |
|---|---|
| author |
Behets, F Disasi, A Ryder, R W Bishagara, K Piot, P Kashamuka, M Kamenga, M Nzila, N Laga, M Vercauteren, G |
| spellingShingle |
Behets, F Disasi, A Ryder, R W Bishagara, K Piot, P Kashamuka, M Kamenga, M Nzila, N Laga, M Vercauteren, G Journal of Clinical Microbiology Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa Microbiology (medical) |
| author_sort |
behets, f |
| spelling |
Behets, F Disasi, A Ryder, R W Bishagara, K Piot, P Kashamuka, M Kamenga, M Nzila, N Laga, M Vercauteren, G 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.29.10.2280-2284.1991 <jats:p>Detection by five different enzyme-linked immunosorbent assays (ELISAs) of antibody to human immunodeficiency virus (HIV) in sera from three Zairian populations consisting of 1,998 individuals with various risks for HIV infection was evaluated. Sera that were reactive by at least one assay and 10% of the nonreactive serum samples were analyzed by Western blot (immunoblot) by using U.S. Public Health Service interpretation criteria. Sera which were positive by ELISA for detection of antibody to HIV-1 and HIV-2 and negative or indeterminate by HIV-1 Western blot were also analyzed by HIV-2 Western blot. Overall, 443 (22.2%) serum specimens were HIV-1 Western blot positive, 390 (19.5%) had indeterminate HIV-1 Western blot patterns, and no samples were HIV-2 Western blot positive. The sensitivity of the ELISAs ranged from 97.5 to 99.8%, and the specificity ranged from 51.7 to 98.4%. By population group, the negative predictive value ranged from 97.1 to 100%, in contrast to the positive predictive value, which varied from 6.6 to 100%. Follow-up results for sera which were indeterminate for antibody to HIV-1 documented only four seroconversions (6.0%) among 67 individuals at high risk for HIV-1 infection and no seroconversions among 202 individuals at relatively low risk for HIV-1 infection. This study demonstrates the importance of evaluating commercial ELISAs with sera from appropriate geographical regions in order to select the most cost-effective and practical assay for use in that region. Furthermore, the high frequency of indeterminate Western blots for African sera emphasizes the continual need for improved confirmatory assays and interpretation criteria.</jats:p> Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa Journal of Clinical Microbiology |
| doi_str_mv |
10.1128/jcm.29.10.2280-2284.1991 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMjkuMTAuMjI4MC0yMjg0LjE5OTE |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMjkuMTAuMjI4MC0yMjg0LjE5OTE |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 |
| imprint |
American Society for Microbiology, 1991 |
| imprint_str_mv |
American Society for Microbiology, 1991 |
| issn |
0095-1137 1098-660X |
| issn_str_mv |
0095-1137 1098-660X |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
behets1991comparisonoffivecommercialenzymelinkedimmunosorbentassaysandwesternimmunoblottingforhumanimmunodeficiencyvirusantibodydetectioninserumsamplesfromcentralafrica |
| publishDateSort |
1991 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Clinical Microbiology |
| source_id |
49 |
| title |
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_unstemmed |
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_full |
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_fullStr |
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_full_unstemmed |
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_short |
Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_sort |
comparison of five commercial enzyme-linked immunosorbent assays and western immunoblotting for human immunodeficiency virus antibody detection in serum samples from central africa |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.29.10.2280-2284.1991 |
| publishDate |
1991 |
| physical |
2280-2284 |
| description |
<jats:p>Detection by five different enzyme-linked immunosorbent assays (ELISAs) of antibody to human immunodeficiency virus (HIV) in sera from three Zairian populations consisting of 1,998 individuals with various risks for HIV infection was evaluated. Sera that were reactive by at least one assay and 10% of the nonreactive serum samples were analyzed by Western blot (immunoblot) by using U.S. Public Health Service interpretation criteria. Sera which were positive by ELISA for detection of antibody to HIV-1 and HIV-2 and negative or indeterminate by HIV-1 Western blot were also analyzed by HIV-2 Western blot. Overall, 443 (22.2%) serum specimens were HIV-1 Western blot positive, 390 (19.5%) had indeterminate HIV-1 Western blot patterns, and no samples were HIV-2 Western blot positive. The sensitivity of the ELISAs ranged from 97.5 to 99.8%, and the specificity ranged from 51.7 to 98.4%. By population group, the negative predictive value ranged from 97.1 to 100%, in contrast to the positive predictive value, which varied from 6.6 to 100%. Follow-up results for sera which were indeterminate for antibody to HIV-1 documented only four seroconversions (6.0%) among 67 individuals at high risk for HIV-1 infection and no seroconversions among 202 individuals at relatively low risk for HIV-1 infection. This study demonstrates the importance of evaluating commercial ELISAs with sera from appropriate geographical regions in order to select the most cost-effective and practical assay for use in that region. Furthermore, the high frequency of indeterminate Western blots for African sera emphasizes the continual need for improved confirmatory assays and interpretation criteria.</jats:p> |
| container_issue |
10 |
| container_start_page |
2280 |
| container_title |
Journal of Clinical Microbiology |
| container_volume |
29 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792336747834638348 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:05:22.341Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Comparison+of+five+commercial+enzyme-linked+immunosorbent+assays+and+Western+immunoblotting+for+human+immunodeficiency+virus+antibody+detection+in+serum+samples+from+Central+Africa&rft.date=1991-10-01&genre=article&issn=1098-660X&volume=29&issue=10&spage=2280&epage=2284&pages=2280-2284&jtitle=Journal+of+Clinical+Microbiology&atitle=Comparison+of+five+commercial+enzyme-linked+immunosorbent+assays+and+Western+immunoblotting+for+human+immunodeficiency+virus+antibody+detection+in+serum+samples+from+Central+Africa&aulast=Vercauteren&aufirst=G&rft_id=info%3Adoi%2F10.1128%2Fjcm.29.10.2280-2284.1991&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792336747834638348 |
| author | Behets, F, Disasi, A, Ryder, R W, Bishagara, K, Piot, P, Kashamuka, M, Kamenga, M, Nzila, N, Laga, M, Vercauteren, G |
| author_facet | Behets, F, Disasi, A, Ryder, R W, Bishagara, K, Piot, P, Kashamuka, M, Kamenga, M, Nzila, N, Laga, M, Vercauteren, G, Behets, F, Disasi, A, Ryder, R W, Bishagara, K, Piot, P, Kashamuka, M, Kamenga, M, Nzila, N, Laga, M, Vercauteren, G |
| author_sort | behets, f |
| container_issue | 10 |
| container_start_page | 2280 |
| container_title | Journal of Clinical Microbiology |
| container_volume | 29 |
| description | <jats:p>Detection by five different enzyme-linked immunosorbent assays (ELISAs) of antibody to human immunodeficiency virus (HIV) in sera from three Zairian populations consisting of 1,998 individuals with various risks for HIV infection was evaluated. Sera that were reactive by at least one assay and 10% of the nonreactive serum samples were analyzed by Western blot (immunoblot) by using U.S. Public Health Service interpretation criteria. Sera which were positive by ELISA for detection of antibody to HIV-1 and HIV-2 and negative or indeterminate by HIV-1 Western blot were also analyzed by HIV-2 Western blot. Overall, 443 (22.2%) serum specimens were HIV-1 Western blot positive, 390 (19.5%) had indeterminate HIV-1 Western blot patterns, and no samples were HIV-2 Western blot positive. The sensitivity of the ELISAs ranged from 97.5 to 99.8%, and the specificity ranged from 51.7 to 98.4%. By population group, the negative predictive value ranged from 97.1 to 100%, in contrast to the positive predictive value, which varied from 6.6 to 100%. Follow-up results for sera which were indeterminate for antibody to HIV-1 documented only four seroconversions (6.0%) among 67 individuals at high risk for HIV-1 infection and no seroconversions among 202 individuals at relatively low risk for HIV-1 infection. This study demonstrates the importance of evaluating commercial ELISAs with sera from appropriate geographical regions in order to select the most cost-effective and practical assay for use in that region. Furthermore, the high frequency of indeterminate Western blots for African sera emphasizes the continual need for improved confirmatory assays and interpretation criteria.</jats:p> |
| doi_str_mv | 10.1128/jcm.29.10.2280-2284.1991 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMjkuMTAuMjI4MC0yMjg0LjE5OTE |
| imprint | American Society for Microbiology, 1991 |
| imprint_str_mv | American Society for Microbiology, 1991 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 0095-1137, 1098-660X |
| issn_str_mv | 0095-1137, 1098-660X |
| language | English |
| last_indexed | 2024-03-01T15:05:22.341Z |
| match_str | behets1991comparisonoffivecommercialenzymelinkedimmunosorbentassaysandwesternimmunoblottingforhumanimmunodeficiencyvirusantibodydetectioninserumsamplesfromcentralafrica |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 2280-2284 |
| publishDate | 1991 |
| publishDateSort | 1991 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Clinical Microbiology |
| source_id | 49 |
| spelling | Behets, F Disasi, A Ryder, R W Bishagara, K Piot, P Kashamuka, M Kamenga, M Nzila, N Laga, M Vercauteren, G 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.29.10.2280-2284.1991 <jats:p>Detection by five different enzyme-linked immunosorbent assays (ELISAs) of antibody to human immunodeficiency virus (HIV) in sera from three Zairian populations consisting of 1,998 individuals with various risks for HIV infection was evaluated. Sera that were reactive by at least one assay and 10% of the nonreactive serum samples were analyzed by Western blot (immunoblot) by using U.S. Public Health Service interpretation criteria. Sera which were positive by ELISA for detection of antibody to HIV-1 and HIV-2 and negative or indeterminate by HIV-1 Western blot were also analyzed by HIV-2 Western blot. Overall, 443 (22.2%) serum specimens were HIV-1 Western blot positive, 390 (19.5%) had indeterminate HIV-1 Western blot patterns, and no samples were HIV-2 Western blot positive. The sensitivity of the ELISAs ranged from 97.5 to 99.8%, and the specificity ranged from 51.7 to 98.4%. By population group, the negative predictive value ranged from 97.1 to 100%, in contrast to the positive predictive value, which varied from 6.6 to 100%. Follow-up results for sera which were indeterminate for antibody to HIV-1 documented only four seroconversions (6.0%) among 67 individuals at high risk for HIV-1 infection and no seroconversions among 202 individuals at relatively low risk for HIV-1 infection. This study demonstrates the importance of evaluating commercial ELISAs with sera from appropriate geographical regions in order to select the most cost-effective and practical assay for use in that region. Furthermore, the high frequency of indeterminate Western blots for African sera emphasizes the continual need for improved confirmatory assays and interpretation criteria.</jats:p> Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa Journal of Clinical Microbiology |
| spellingShingle | Behets, F, Disasi, A, Ryder, R W, Bishagara, K, Piot, P, Kashamuka, M, Kamenga, M, Nzila, N, Laga, M, Vercauteren, G, Journal of Clinical Microbiology, Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa, Microbiology (medical) |
| title | Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_full | Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_fullStr | Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_full_unstemmed | Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_short | Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| title_sort | comparison of five commercial enzyme-linked immunosorbent assays and western immunoblotting for human immunodeficiency virus antibody detection in serum samples from central africa |
| title_unstemmed | Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.29.10.2280-2284.1991 |