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Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Clinical Microbiology, 21, 1985, 1, S. 88-91 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Merli, A Canessa, A Melioli, G Merli, A Canessa, A Melioli, G |
|---|---|
| author |
Merli, A Canessa, A Melioli, G |
| spellingShingle |
Merli, A Canessa, A Melioli, G Journal of Clinical Microbiology Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture Microbiology (medical) |
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merli, a |
| spelling |
Merli, A Canessa, A Melioli, G 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.21.1.88-91.1985 <jats:p>An enzyme-linked immunosorbent assay (ELISA) for the evaluation of growth kinetics of Toxoplasma gondii in tissue cultures was developed. Tissue culture microplates (96 wells) were seeded with Vero cells, infected with a virulent strain of T. gondii, and incubated for different time periods. The ELISA was performed with anti-T. gondii antibodies on the infected cells. The method was simple, rapid, and accurate, and very good correlations between the ELISA results and the percentage of T. gondii rosettes in infected cells, the number of free T. gondii in the supernatant, and the amount of T. gondii antigen in the supernatant were observed.</jats:p> Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture Journal of Clinical Microbiology |
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American Society for Microbiology, 1985 |
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American Society for Microbiology, 1985 |
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1985 |
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American Society for Microbiology |
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Journal of Clinical Microbiology |
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| title |
Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_unstemmed |
Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_full |
Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_fullStr |
Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_full_unstemmed |
Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_short |
Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_sort |
enzyme immunoassay for evaluation of toxoplasma gondii growth in tissue culture |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.21.1.88-91.1985 |
| publishDate |
1985 |
| physical |
88-91 |
| description |
<jats:p>An enzyme-linked immunosorbent assay (ELISA) for the evaluation of growth kinetics of Toxoplasma gondii in tissue cultures was developed. Tissue culture microplates (96 wells) were seeded with Vero cells, infected with a virulent strain of T. gondii, and incubated for different time periods. The ELISA was performed with anti-T. gondii antibodies on the infected cells. The method was simple, rapid, and accurate, and very good correlations between the ELISA results and the percentage of T. gondii rosettes in infected cells, the number of free T. gondii in the supernatant, and the amount of T. gondii antigen in the supernatant were observed.</jats:p> |
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| author | Merli, A, Canessa, A, Melioli, G |
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| description | <jats:p>An enzyme-linked immunosorbent assay (ELISA) for the evaluation of growth kinetics of Toxoplasma gondii in tissue cultures was developed. Tissue culture microplates (96 wells) were seeded with Vero cells, infected with a virulent strain of T. gondii, and incubated for different time periods. The ELISA was performed with anti-T. gondii antibodies on the infected cells. The method was simple, rapid, and accurate, and very good correlations between the ELISA results and the percentage of T. gondii rosettes in infected cells, the number of free T. gondii in the supernatant, and the amount of T. gondii antigen in the supernatant were observed.</jats:p> |
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| imprint | American Society for Microbiology, 1985 |
| imprint_str_mv | American Society for Microbiology, 1985 |
| institution | DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| physical | 88-91 |
| publishDate | 1985 |
| publishDateSort | 1985 |
| publisher | American Society for Microbiology |
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| recordtype | ai |
| series | Journal of Clinical Microbiology |
| source_id | 49 |
| spelling | Merli, A Canessa, A Melioli, G 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.21.1.88-91.1985 <jats:p>An enzyme-linked immunosorbent assay (ELISA) for the evaluation of growth kinetics of Toxoplasma gondii in tissue cultures was developed. Tissue culture microplates (96 wells) were seeded with Vero cells, infected with a virulent strain of T. gondii, and incubated for different time periods. The ELISA was performed with anti-T. gondii antibodies on the infected cells. The method was simple, rapid, and accurate, and very good correlations between the ELISA results and the percentage of T. gondii rosettes in infected cells, the number of free T. gondii in the supernatant, and the amount of T. gondii antigen in the supernatant were observed.</jats:p> Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture Journal of Clinical Microbiology |
| spellingShingle | Merli, A, Canessa, A, Melioli, G, Journal of Clinical Microbiology, Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture, Microbiology (medical) |
| title | Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_full | Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_fullStr | Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_full_unstemmed | Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_short | Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| title_sort | enzyme immunoassay for evaluation of toxoplasma gondii growth in tissue culture |
| title_unstemmed | Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.21.1.88-91.1985 |