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Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Clinical Microbiology, 50, 2012, 12, S. 4147-4150 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
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Hasan, Mohammad R. Tan, Rusung Al-Rawahi, Ghada N. Thomas, Eva Tilley, Peter Hasan, Mohammad R. Tan, Rusung Al-Rawahi, Ghada N. Thomas, Eva Tilley, Peter |
|---|---|
| author |
Hasan, Mohammad R. Tan, Rusung Al-Rawahi, Ghada N. Thomas, Eva Tilley, Peter |
| spellingShingle |
Hasan, Mohammad R. Tan, Rusung Al-Rawahi, Ghada N. Thomas, Eva Tilley, Peter Journal of Clinical Microbiology Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples Microbiology (medical) |
| author_sort |
hasan, mohammad r. |
| spelling |
Hasan, Mohammad R. Tan, Rusung Al-Rawahi, Ghada N. Thomas, Eva Tilley, Peter 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.02659-12 <jats:title>ABSTRACT</jats:title> <jats:p> The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and −80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets ( <jats:italic>P</jats:italic> < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples. </jats:p> Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples Journal of Clinical Microbiology |
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| title |
Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_unstemmed |
Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_full |
Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_fullStr |
Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
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Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_short |
Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_sort |
short-term stability of pathogen-specific nucleic acid targets in clinical samples |
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Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.02659-12 |
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2012 |
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4147-4150 |
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<jats:title>ABSTRACT</jats:title>
<jats:p>
The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and −80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets (
<jats:italic>P</jats:italic>
< 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples.
</jats:p> |
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| author | Hasan, Mohammad R., Tan, Rusung, Al-Rawahi, Ghada N., Thomas, Eva, Tilley, Peter |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and −80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets ( <jats:italic>P</jats:italic> < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples. </jats:p> |
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| spelling | Hasan, Mohammad R. Tan, Rusung Al-Rawahi, Ghada N. Thomas, Eva Tilley, Peter 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.02659-12 <jats:title>ABSTRACT</jats:title> <jats:p> The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and −80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets ( <jats:italic>P</jats:italic> < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples. </jats:p> Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples Journal of Clinical Microbiology |
| spellingShingle | Hasan, Mohammad R., Tan, Rusung, Al-Rawahi, Ghada N., Thomas, Eva, Tilley, Peter, Journal of Clinical Microbiology, Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples, Microbiology (medical) |
| title | Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_full | Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_fullStr | Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_full_unstemmed | Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_short | Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| title_sort | short-term stability of pathogen-specific nucleic acid targets in clinical samples |
| title_unstemmed | Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.02659-12 |