Eintrag weiter verarbeiten
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis
Gespeichert in:
| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Clinical Microbiology, 56, 2018, 4 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Liesman, Rachael M. Strasburg, Angela P. Heitman, Angela K. Theel, Elitza S. Patel, Robin Binnicker, Matthew J. Liesman, Rachael M. Strasburg, Angela P. Heitman, Angela K. Theel, Elitza S. Patel, Robin Binnicker, Matthew J. |
|---|---|
| author |
Liesman, Rachael M. Strasburg, Angela P. Heitman, Angela K. Theel, Elitza S. Patel, Robin Binnicker, Matthew J. |
| spellingShingle |
Liesman, Rachael M. Strasburg, Angela P. Heitman, Angela K. Theel, Elitza S. Patel, Robin Binnicker, Matthew J. Journal of Clinical Microbiology Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis Microbiology (medical) |
| author_sort |
liesman, rachael m. |
| spelling |
Liesman, Rachael M. Strasburg, Angela P. Heitman, Angela K. Theel, Elitza S. Patel, Robin Binnicker, Matthew J. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.01927-17 <jats:title>ABSTRACT</jats:title> <jats:p> Rapid and accurate laboratory tests are important for the timely diagnosis and treatment of central nervous system infections. The FilmArray meningitis/encephalitis (ME) panel (BioFire Diagnostics, Salt Lake City, UT) is an FDA-cleared, multiplex molecular panel that allows the detection of 14 pathogens (bacterial [ <jats:italic>n</jats:italic> = 6], viral [ <jats:italic>n</jats:italic> = 7], and fungal [ <jats:italic>n</jats:italic> = 1] pathogens) from cerebrospinal fluid (CSF). In this study, we evaluated the performance characteristics of the FilmArray ME panel using clinical, residual CSF samples ( <jats:italic>n</jats:italic> = 291) that tested positive by a routine method(s) (e.g., bacterial culture, individual real-time PCR assay) for a pathogen represented on the ME panel. Of note, a subset ( <jats:italic>n</jats:italic> = 76) of the CSF specimens was collected during the prevaccine era and had been characterized as positive for a bacterial pathogen. The FilmArray ME panel demonstrated an overall percent positive agreement (PPA) of 97.5% (78/80) for bacterial pathogens, 90.1% (145/161) for viruses, and 52% (26/50) for <jats:named-content content-type="genus-species">Cryptococcus</jats:named-content> <jats:italic>neoformans/C. gattii</jats:italic> . Despite the low overall agreement (52%) between the ME panel and antigen testing for detection of <jats:italic>C. neoformans/C. gattii</jats:italic> , the percent positive agreement of the FilmArray assay for <jats:italic>C. neoformans/C. gattii</jats:italic> was 92.3% (12/13) when the results were compared directly to the results of routine fungal smear or culture. The FilmArray ME panel offers a rapid (∼60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens. </jats:p> Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis Journal of Clinical Microbiology |
| doi_str_mv |
10.1128/jcm.01927-17 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMDE5MjctMTc |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMDE5MjctMTc |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
American Society for Microbiology, 2018 |
| imprint_str_mv |
American Society for Microbiology, 2018 |
| issn |
0095-1137 1098-660X |
| issn_str_mv |
0095-1137 1098-660X |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
liesman2018evaluationofacommercialmultiplexmolecularpanelfordiagnosisofinfectiousmeningitisandencephalitis |
| publishDateSort |
2018 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Clinical Microbiology |
| source_id |
49 |
| title |
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_unstemmed |
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_full |
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_fullStr |
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_full_unstemmed |
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_short |
Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_sort |
evaluation of a commercial multiplex molecular panel for diagnosis of infectious meningitis and encephalitis |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.01927-17 |
| publishDate |
2018 |
| physical |
|
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Rapid and accurate laboratory tests are important for the timely diagnosis and treatment of central nervous system infections. The FilmArray meningitis/encephalitis (ME) panel (BioFire Diagnostics, Salt Lake City, UT) is an FDA-cleared, multiplex molecular panel that allows the detection of 14 pathogens (bacterial [
<jats:italic>n</jats:italic>
= 6], viral [
<jats:italic>n</jats:italic>
= 7], and fungal [
<jats:italic>n</jats:italic>
= 1] pathogens) from cerebrospinal fluid (CSF). In this study, we evaluated the performance characteristics of the FilmArray ME panel using clinical, residual CSF samples (
<jats:italic>n</jats:italic>
= 291) that tested positive by a routine method(s) (e.g., bacterial culture, individual real-time PCR assay) for a pathogen represented on the ME panel. Of note, a subset (
<jats:italic>n</jats:italic>
= 76) of the CSF specimens was collected during the prevaccine era and had been characterized as positive for a bacterial pathogen. The FilmArray ME panel demonstrated an overall percent positive agreement (PPA) of 97.5% (78/80) for bacterial pathogens, 90.1% (145/161) for viruses, and 52% (26/50) for
<jats:named-content content-type="genus-species">Cryptococcus</jats:named-content>
<jats:italic>neoformans/C. gattii</jats:italic>
. Despite the low overall agreement (52%) between the ME panel and antigen testing for detection of
<jats:italic>C. neoformans/C. gattii</jats:italic>
, the percent positive agreement of the FilmArray assay for
<jats:italic>C. neoformans/C. gattii</jats:italic>
was 92.3% (12/13) when the results were compared directly to the results of routine fungal smear or culture. The FilmArray ME panel offers a rapid (∼60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens.
</jats:p> |
| container_issue |
4 |
| container_start_page |
0 |
| container_title |
Journal of Clinical Microbiology |
| container_volume |
56 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346199360012290 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:35:35.674Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Evaluation+of+a+Commercial+Multiplex+Molecular+Panel+for+Diagnosis+of+Infectious+Meningitis+and+Encephalitis&rft.date=2018-04-01&genre=article&issn=1098-660X&volume=56&issue=4&jtitle=Journal+of+Clinical+Microbiology&atitle=Evaluation+of+a+Commercial+Multiplex+Molecular+Panel+for+Diagnosis+of+Infectious+Meningitis+and+Encephalitis&aulast=Binnicker&aufirst=Matthew+J.&rft_id=info%3Adoi%2F10.1128%2Fjcm.01927-17&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346199360012290 |
| author | Liesman, Rachael M., Strasburg, Angela P., Heitman, Angela K., Theel, Elitza S., Patel, Robin, Binnicker, Matthew J. |
| author_facet | Liesman, Rachael M., Strasburg, Angela P., Heitman, Angela K., Theel, Elitza S., Patel, Robin, Binnicker, Matthew J., Liesman, Rachael M., Strasburg, Angela P., Heitman, Angela K., Theel, Elitza S., Patel, Robin, Binnicker, Matthew J. |
| author_sort | liesman, rachael m. |
| container_issue | 4 |
| container_start_page | 0 |
| container_title | Journal of Clinical Microbiology |
| container_volume | 56 |
| description | <jats:title>ABSTRACT</jats:title> <jats:p> Rapid and accurate laboratory tests are important for the timely diagnosis and treatment of central nervous system infections. The FilmArray meningitis/encephalitis (ME) panel (BioFire Diagnostics, Salt Lake City, UT) is an FDA-cleared, multiplex molecular panel that allows the detection of 14 pathogens (bacterial [ <jats:italic>n</jats:italic> = 6], viral [ <jats:italic>n</jats:italic> = 7], and fungal [ <jats:italic>n</jats:italic> = 1] pathogens) from cerebrospinal fluid (CSF). In this study, we evaluated the performance characteristics of the FilmArray ME panel using clinical, residual CSF samples ( <jats:italic>n</jats:italic> = 291) that tested positive by a routine method(s) (e.g., bacterial culture, individual real-time PCR assay) for a pathogen represented on the ME panel. Of note, a subset ( <jats:italic>n</jats:italic> = 76) of the CSF specimens was collected during the prevaccine era and had been characterized as positive for a bacterial pathogen. The FilmArray ME panel demonstrated an overall percent positive agreement (PPA) of 97.5% (78/80) for bacterial pathogens, 90.1% (145/161) for viruses, and 52% (26/50) for <jats:named-content content-type="genus-species">Cryptococcus</jats:named-content> <jats:italic>neoformans/C. gattii</jats:italic> . Despite the low overall agreement (52%) between the ME panel and antigen testing for detection of <jats:italic>C. neoformans/C. gattii</jats:italic> , the percent positive agreement of the FilmArray assay for <jats:italic>C. neoformans/C. gattii</jats:italic> was 92.3% (12/13) when the results were compared directly to the results of routine fungal smear or culture. The FilmArray ME panel offers a rapid (∼60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens. </jats:p> |
| doi_str_mv | 10.1128/jcm.01927-17 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMDE5MjctMTc |
| imprint | American Society for Microbiology, 2018 |
| imprint_str_mv | American Society for Microbiology, 2018 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0095-1137, 1098-660X |
| issn_str_mv | 0095-1137, 1098-660X |
| language | English |
| last_indexed | 2024-03-01T17:35:35.674Z |
| match_str | liesman2018evaluationofacommercialmultiplexmolecularpanelfordiagnosisofinfectiousmeningitisandencephalitis |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Clinical Microbiology |
| source_id | 49 |
| spelling | Liesman, Rachael M. Strasburg, Angela P. Heitman, Angela K. Theel, Elitza S. Patel, Robin Binnicker, Matthew J. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.01927-17 <jats:title>ABSTRACT</jats:title> <jats:p> Rapid and accurate laboratory tests are important for the timely diagnosis and treatment of central nervous system infections. The FilmArray meningitis/encephalitis (ME) panel (BioFire Diagnostics, Salt Lake City, UT) is an FDA-cleared, multiplex molecular panel that allows the detection of 14 pathogens (bacterial [ <jats:italic>n</jats:italic> = 6], viral [ <jats:italic>n</jats:italic> = 7], and fungal [ <jats:italic>n</jats:italic> = 1] pathogens) from cerebrospinal fluid (CSF). In this study, we evaluated the performance characteristics of the FilmArray ME panel using clinical, residual CSF samples ( <jats:italic>n</jats:italic> = 291) that tested positive by a routine method(s) (e.g., bacterial culture, individual real-time PCR assay) for a pathogen represented on the ME panel. Of note, a subset ( <jats:italic>n</jats:italic> = 76) of the CSF specimens was collected during the prevaccine era and had been characterized as positive for a bacterial pathogen. The FilmArray ME panel demonstrated an overall percent positive agreement (PPA) of 97.5% (78/80) for bacterial pathogens, 90.1% (145/161) for viruses, and 52% (26/50) for <jats:named-content content-type="genus-species">Cryptococcus</jats:named-content> <jats:italic>neoformans/C. gattii</jats:italic> . Despite the low overall agreement (52%) between the ME panel and antigen testing for detection of <jats:italic>C. neoformans/C. gattii</jats:italic> , the percent positive agreement of the FilmArray assay for <jats:italic>C. neoformans/C. gattii</jats:italic> was 92.3% (12/13) when the results were compared directly to the results of routine fungal smear or culture. The FilmArray ME panel offers a rapid (∼60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens. </jats:p> Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis Journal of Clinical Microbiology |
| spellingShingle | Liesman, Rachael M., Strasburg, Angela P., Heitman, Angela K., Theel, Elitza S., Patel, Robin, Binnicker, Matthew J., Journal of Clinical Microbiology, Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis, Microbiology (medical) |
| title | Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_full | Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_fullStr | Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_full_unstemmed | Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_short | Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| title_sort | evaluation of a commercial multiplex molecular panel for diagnosis of infectious meningitis and encephalitis |
| title_unstemmed | Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.01927-17 |