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Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet?
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Clinical Microbiology, 55, 2017, 5, S. 1249-1254 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Lee, Robyn S. Pai, Madhukar Lee, Robyn S. Pai, Madhukar |
|---|---|
| author |
Lee, Robyn S. Pai, Madhukar |
| spellingShingle |
Lee, Robyn S. Pai, Madhukar Journal of Clinical Microbiology Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? Microbiology (medical) |
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lee, robyn s. |
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Lee, Robyn S. Pai, Madhukar 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00358-17 <jats:title>ABSTRACT</jats:title> <jats:p> Whole-genome sequencing has taken a leading role in epidemiologic studies of tuberculosis, but thus far, its real-time clinical utility has been low, in part because of the requirement for culture. In their report in this issue, Votintseva et al. (A. A. Votintseva, P. Bradley, L. Pankhurst, C. del Ojo Elias, M. Loose, K. Nilgiriwala, A. Chatterjee, E. G. Smith, N. Sanderson, T. M. Walker, M. R. Morgan, D. H. Wyllie, A. S. Walker, T. E. A. Peto, D. W. Crook, and Z. Iqbal, J Clin Microbiol 55:1285–1298, 2017, <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://doi.org/10.1128/JCM.02483-16">https://doi.org/10.1128/JCM.02483-16</jats:ext-link> ) present a new method for extracting <jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content> DNA directly from smear-positive respiratory samples, making it feasible to generate drug resistance predictions and phylogenetic trees in 44 h with the Illumina MiSeq. They also illustrate the potential for a <24-h turnaround time from DNA extraction to clinically relevant results with Illumina MiniSeq and Oxford Nanopore Technologies MinION. We comment on the promise and limitations of these approaches. </jats:p> Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? Journal of Clinical Microbiology |
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Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
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Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
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Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
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Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
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Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_short |
Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_sort |
real-time sequencing of mycobacterium tuberculosis: are we there yet? |
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Microbiology (medical) |
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http://dx.doi.org/10.1128/jcm.00358-17 |
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2017 |
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1249-1254 |
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<jats:title>ABSTRACT</jats:title>
<jats:p>
Whole-genome sequencing has taken a leading role in epidemiologic studies of tuberculosis, but thus far, its real-time clinical utility has been low, in part because of the requirement for culture. In their report in this issue, Votintseva et al. (A. A. Votintseva, P. Bradley, L. Pankhurst, C. del Ojo Elias, M. Loose, K. Nilgiriwala, A. Chatterjee, E. G. Smith, N. Sanderson, T. M. Walker, M. R. Morgan, D. H. Wyllie, A. S. Walker, T. E. A. Peto, D. W. Crook, and Z. Iqbal, J Clin Microbiol 55:1285–1298, 2017,
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://doi.org/10.1128/JCM.02483-16">https://doi.org/10.1128/JCM.02483-16</jats:ext-link>
) present a new method for extracting
<jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content>
DNA directly from smear-positive respiratory samples, making it feasible to generate drug resistance predictions and phylogenetic trees in 44 h with the Illumina MiSeq. They also illustrate the potential for a <24-h turnaround time from DNA extraction to clinically relevant results with Illumina MiniSeq and Oxford Nanopore Technologies MinION. We comment on the promise and limitations of these approaches.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Whole-genome sequencing has taken a leading role in epidemiologic studies of tuberculosis, but thus far, its real-time clinical utility has been low, in part because of the requirement for culture. In their report in this issue, Votintseva et al. (A. A. Votintseva, P. Bradley, L. Pankhurst, C. del Ojo Elias, M. Loose, K. Nilgiriwala, A. Chatterjee, E. G. Smith, N. Sanderson, T. M. Walker, M. R. Morgan, D. H. Wyllie, A. S. Walker, T. E. A. Peto, D. W. Crook, and Z. Iqbal, J Clin Microbiol 55:1285–1298, 2017, <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://doi.org/10.1128/JCM.02483-16">https://doi.org/10.1128/JCM.02483-16</jats:ext-link> ) present a new method for extracting <jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content> DNA directly from smear-positive respiratory samples, making it feasible to generate drug resistance predictions and phylogenetic trees in 44 h with the Illumina MiSeq. They also illustrate the potential for a <24-h turnaround time from DNA extraction to clinically relevant results with Illumina MiniSeq and Oxford Nanopore Technologies MinION. We comment on the promise and limitations of these approaches. </jats:p> |
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| spelling | Lee, Robyn S. Pai, Madhukar 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00358-17 <jats:title>ABSTRACT</jats:title> <jats:p> Whole-genome sequencing has taken a leading role in epidemiologic studies of tuberculosis, but thus far, its real-time clinical utility has been low, in part because of the requirement for culture. In their report in this issue, Votintseva et al. (A. A. Votintseva, P. Bradley, L. Pankhurst, C. del Ojo Elias, M. Loose, K. Nilgiriwala, A. Chatterjee, E. G. Smith, N. Sanderson, T. M. Walker, M. R. Morgan, D. H. Wyllie, A. S. Walker, T. E. A. Peto, D. W. Crook, and Z. Iqbal, J Clin Microbiol 55:1285–1298, 2017, <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://doi.org/10.1128/JCM.02483-16">https://doi.org/10.1128/JCM.02483-16</jats:ext-link> ) present a new method for extracting <jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content> DNA directly from smear-positive respiratory samples, making it feasible to generate drug resistance predictions and phylogenetic trees in 44 h with the Illumina MiSeq. They also illustrate the potential for a <24-h turnaround time from DNA extraction to clinically relevant results with Illumina MiniSeq and Oxford Nanopore Technologies MinION. We comment on the promise and limitations of these approaches. </jats:p> Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? Journal of Clinical Microbiology |
| spellingShingle | Lee, Robyn S., Pai, Madhukar, Journal of Clinical Microbiology, Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet?, Microbiology (medical) |
| title | Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_full | Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_fullStr | Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_full_unstemmed | Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_short | Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| title_sort | real-time sequencing of mycobacterium tuberculosis: are we there yet? |
| title_unstemmed | Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet? |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.00358-17 |