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Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Clinical Microbiology, 47, 2009, 7, S. 2304-2306 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Lan, Xi Yang, Bin Li, Bao Yu Yin, Xiang Ping Li, Xue Rui Liu, Ji Xing Lan, Xi Yang, Bin Li, Bao Yu Yin, Xiang Ping Li, Xue Rui Liu, Ji Xing |
|---|---|
| author |
Lan, Xi Yang, Bin Li, Bao Yu Yin, Xiang Ping Li, Xue Rui Liu, Ji Xing |
| spellingShingle |
Lan, Xi Yang, Bin Li, Bao Yu Yin, Xiang Ping Li, Xue Rui Liu, Ji Xing Journal of Clinical Microbiology Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus Microbiology (medical) |
| author_sort |
lan, xi |
| spelling |
Lan, Xi Yang, Bin Li, Bao Yu Yin, Xiang Ping Li, Xue Rui Liu, Ji Xing 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00498-09 <jats:title>ABSTRACT</jats:title> <jats:p>The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63°C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).</jats:p> Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus Journal of Clinical Microbiology |
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American Society for Microbiology, 2009 |
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| title |
Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_unstemmed |
Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_full |
Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_fullStr |
Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_full_unstemmed |
Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_short |
Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_sort |
reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis e virus |
| topic |
Microbiology (medical) |
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http://dx.doi.org/10.1128/jcm.00498-09 |
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2009 |
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2304-2306 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63°C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).</jats:p> |
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| author | Lan, Xi, Yang, Bin, Li, Bao Yu, Yin, Xiang Ping, Li, Xue Rui, Liu, Ji Xing |
| author_facet | Lan, Xi, Yang, Bin, Li, Bao Yu, Yin, Xiang Ping, Li, Xue Rui, Liu, Ji Xing, Lan, Xi, Yang, Bin, Li, Bao Yu, Yin, Xiang Ping, Li, Xue Rui, Liu, Ji Xing |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p>The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63°C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).</jats:p> |
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| spelling | Lan, Xi Yang, Bin Li, Bao Yu Yin, Xiang Ping Li, Xue Rui Liu, Ji Xing 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00498-09 <jats:title>ABSTRACT</jats:title> <jats:p>The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63°C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).</jats:p> Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus Journal of Clinical Microbiology |
| spellingShingle | Lan, Xi, Yang, Bin, Li, Bao Yu, Yin, Xiang Ping, Li, Xue Rui, Liu, Ji Xing, Journal of Clinical Microbiology, Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus, Microbiology (medical) |
| title | Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_full | Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_fullStr | Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_full_unstemmed | Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_short | Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| title_sort | reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis e virus |
| title_unstemmed | Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.00498-09 |