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Murine Macrophages Kill the Vegetative Form of Bacillus anthracis
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| Zeitschriftentitel: | Infection and Immunity |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Infection and Immunity, 73, 2005, 11, S. 7495-7501 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Kang, Tae Jin Fenton, Matthew J. Weiner, Matthew A. Hibbs, Stephen Basu, Subhendu Baillie, Les Cross, Alan S. Kang, Tae Jin Fenton, Matthew J. Weiner, Matthew A. Hibbs, Stephen Basu, Subhendu Baillie, Les Cross, Alan S. |
|---|---|
| author |
Kang, Tae Jin Fenton, Matthew J. Weiner, Matthew A. Hibbs, Stephen Basu, Subhendu Baillie, Les Cross, Alan S. |
| spellingShingle |
Kang, Tae Jin Fenton, Matthew J. Weiner, Matthew A. Hibbs, Stephen Basu, Subhendu Baillie, Les Cross, Alan S. Infection and Immunity Murine Macrophages Kill the Vegetative Form of Bacillus anthracis Infectious Diseases Immunology Microbiology Parasitology |
| author_sort |
kang, tae jin |
| spelling |
Kang, Tae Jin Fenton, Matthew J. Weiner, Matthew A. Hibbs, Stephen Basu, Subhendu Baillie, Les Cross, Alan S. 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.73.11.7495-7501.2005 <jats:title>ABSTRACT</jats:title> <jats:p> Anti-protective antigen antibody was reported to enhance macrophage killing of ingested <jats:italic>Bacillus anthracis</jats:italic> spores, but it was unclear whether the antibody-mediated macrophage killing mechanism was directed against the spore itself or the vegetative form emerging from the ingested and germinating spore. To address this question, we compared the killing of germination-proficient (gp) and germination-deficient (Δ <jats:italic>gerH</jats:italic> ) Sterne 34F2 strain spores by murine peritoneal macrophages. While macrophages similarly ingested both spores, only gp Sterne was killed at 5 h (0.37 log kill). Pretreatment of macrophages with gamma interferon (IFN-γ) or opsonization with immunoglobulin G (IgG) isolated from a subject immunized with an anthrax vaccine enhanced the killing of Sterne to 0.49 and 0.73 log, respectively, but the combination of IFN-γ and IgG was no better than either treatment alone. Under no condition was there killing of Δ <jats:italic>gerH</jats:italic> spores. To examine the ability of the exosporium to protect spores from macrophages, we compared the macrophage-mediated killing of nonsonicated (exosporium <jats:sup>+</jats:sup> ) and sonicated (exosporium <jats:sup>−</jats:sup> ) Sterne 34F2 spores. More sonicated spores than nonsonicated spores were killed at 5 h (0.98 versus 0.37 log kill, respectively). Pretreatment with IFN-γ increased the sonicated spore killing to 1.39 log. However, the opsonization with IgG was no better than no treatment or pretreatment with IFN-γ. We conclude that macrophages appear unable to kill the spore form of <jats:italic>B. anthracis</jats:italic> and that the exosporium may play a role in the protection of spores from macrophages. </jats:p> Murine Macrophages Kill the Vegetative Form of <i>Bacillus anthracis</i> Infection and Immunity |
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10.1128/iai.73.11.7495-7501.2005 |
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| title |
Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_unstemmed |
Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_full |
Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_fullStr |
Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_full_unstemmed |
Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_short |
Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_sort |
murine macrophages kill the vegetative form of
<i>bacillus anthracis</i> |
| topic |
Infectious Diseases Immunology Microbiology Parasitology |
| url |
http://dx.doi.org/10.1128/iai.73.11.7495-7501.2005 |
| publishDate |
2005 |
| physical |
7495-7501 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Anti-protective antigen antibody was reported to enhance macrophage killing of ingested
<jats:italic>Bacillus anthracis</jats:italic>
spores, but it was unclear whether the antibody-mediated macrophage killing mechanism was directed against the spore itself or the vegetative form emerging from the ingested and germinating spore. To address this question, we compared the killing of germination-proficient (gp) and germination-deficient (Δ
<jats:italic>gerH</jats:italic>
) Sterne 34F2 strain spores by murine peritoneal macrophages. While macrophages similarly ingested both spores, only gp Sterne was killed at 5 h (0.37 log kill). Pretreatment of macrophages with gamma interferon (IFN-γ) or opsonization with immunoglobulin G (IgG) isolated from a subject immunized with an anthrax vaccine enhanced the killing of Sterne to 0.49 and 0.73 log, respectively, but the combination of IFN-γ and IgG was no better than either treatment alone. Under no condition was there killing of Δ
<jats:italic>gerH</jats:italic>
spores. To examine the ability of the exosporium to protect spores from macrophages, we compared the macrophage-mediated killing of nonsonicated (exosporium
<jats:sup>+</jats:sup>
) and sonicated (exosporium
<jats:sup>−</jats:sup>
) Sterne 34F2 spores. More sonicated spores than nonsonicated spores were killed at 5 h (0.98 versus 0.37 log kill, respectively). Pretreatment with IFN-γ increased the sonicated spore killing to 1.39 log. However, the opsonization with IgG was no better than no treatment or pretreatment with IFN-γ. We conclude that macrophages appear unable to kill the spore form of
<jats:italic>B. anthracis</jats:italic>
and that the exosporium may play a role in the protection of spores from macrophages.
</jats:p> |
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| author | Kang, Tae Jin, Fenton, Matthew J., Weiner, Matthew A., Hibbs, Stephen, Basu, Subhendu, Baillie, Les, Cross, Alan S. |
| author_facet | Kang, Tae Jin, Fenton, Matthew J., Weiner, Matthew A., Hibbs, Stephen, Basu, Subhendu, Baillie, Les, Cross, Alan S., Kang, Tae Jin, Fenton, Matthew J., Weiner, Matthew A., Hibbs, Stephen, Basu, Subhendu, Baillie, Les, Cross, Alan S. |
| author_sort | kang, tae jin |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Anti-protective antigen antibody was reported to enhance macrophage killing of ingested <jats:italic>Bacillus anthracis</jats:italic> spores, but it was unclear whether the antibody-mediated macrophage killing mechanism was directed against the spore itself or the vegetative form emerging from the ingested and germinating spore. To address this question, we compared the killing of germination-proficient (gp) and germination-deficient (Δ <jats:italic>gerH</jats:italic> ) Sterne 34F2 strain spores by murine peritoneal macrophages. While macrophages similarly ingested both spores, only gp Sterne was killed at 5 h (0.37 log kill). Pretreatment of macrophages with gamma interferon (IFN-γ) or opsonization with immunoglobulin G (IgG) isolated from a subject immunized with an anthrax vaccine enhanced the killing of Sterne to 0.49 and 0.73 log, respectively, but the combination of IFN-γ and IgG was no better than either treatment alone. Under no condition was there killing of Δ <jats:italic>gerH</jats:italic> spores. To examine the ability of the exosporium to protect spores from macrophages, we compared the macrophage-mediated killing of nonsonicated (exosporium <jats:sup>+</jats:sup> ) and sonicated (exosporium <jats:sup>−</jats:sup> ) Sterne 34F2 spores. More sonicated spores than nonsonicated spores were killed at 5 h (0.98 versus 0.37 log kill, respectively). Pretreatment with IFN-γ increased the sonicated spore killing to 1.39 log. However, the opsonization with IgG was no better than no treatment or pretreatment with IFN-γ. We conclude that macrophages appear unable to kill the spore form of <jats:italic>B. anthracis</jats:italic> and that the exosporium may play a role in the protection of spores from macrophages. </jats:p> |
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| imprint | American Society for Microbiology, 2005 |
| imprint_str_mv | American Society for Microbiology, 2005 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, FID-BBI-DE-23, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0019-9567, 1098-5522 |
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| language | English |
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| physical | 7495-7501 |
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| spelling | Kang, Tae Jin Fenton, Matthew J. Weiner, Matthew A. Hibbs, Stephen Basu, Subhendu Baillie, Les Cross, Alan S. 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.73.11.7495-7501.2005 <jats:title>ABSTRACT</jats:title> <jats:p> Anti-protective antigen antibody was reported to enhance macrophage killing of ingested <jats:italic>Bacillus anthracis</jats:italic> spores, but it was unclear whether the antibody-mediated macrophage killing mechanism was directed against the spore itself or the vegetative form emerging from the ingested and germinating spore. To address this question, we compared the killing of germination-proficient (gp) and germination-deficient (Δ <jats:italic>gerH</jats:italic> ) Sterne 34F2 strain spores by murine peritoneal macrophages. While macrophages similarly ingested both spores, only gp Sterne was killed at 5 h (0.37 log kill). Pretreatment of macrophages with gamma interferon (IFN-γ) or opsonization with immunoglobulin G (IgG) isolated from a subject immunized with an anthrax vaccine enhanced the killing of Sterne to 0.49 and 0.73 log, respectively, but the combination of IFN-γ and IgG was no better than either treatment alone. Under no condition was there killing of Δ <jats:italic>gerH</jats:italic> spores. To examine the ability of the exosporium to protect spores from macrophages, we compared the macrophage-mediated killing of nonsonicated (exosporium <jats:sup>+</jats:sup> ) and sonicated (exosporium <jats:sup>−</jats:sup> ) Sterne 34F2 spores. More sonicated spores than nonsonicated spores were killed at 5 h (0.98 versus 0.37 log kill, respectively). Pretreatment with IFN-γ increased the sonicated spore killing to 1.39 log. However, the opsonization with IgG was no better than no treatment or pretreatment with IFN-γ. We conclude that macrophages appear unable to kill the spore form of <jats:italic>B. anthracis</jats:italic> and that the exosporium may play a role in the protection of spores from macrophages. </jats:p> Murine Macrophages Kill the Vegetative Form of <i>Bacillus anthracis</i> Infection and Immunity |
| spellingShingle | Kang, Tae Jin, Fenton, Matthew J., Weiner, Matthew A., Hibbs, Stephen, Basu, Subhendu, Baillie, Les, Cross, Alan S., Infection and Immunity, Murine Macrophages Kill the Vegetative Form of Bacillus anthracis, Infectious Diseases, Immunology, Microbiology, Parasitology |
| title | Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_full | Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_fullStr | Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_full_unstemmed | Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_short | Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| title_sort | murine macrophages kill the vegetative form of <i>bacillus anthracis</i> |
| title_unstemmed | Murine Macrophages Kill the Vegetative Form of Bacillus anthracis |
| topic | Infectious Diseases, Immunology, Microbiology, Parasitology |
| url | http://dx.doi.org/10.1128/iai.73.11.7495-7501.2005 |