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Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Applied and Environmental Microbiology, 72, 2006, 3, S. 1939-1948 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Lee, Sang Jun Song, Hyohak Lee, Sang Yup Lee, Sang Jun Song, Hyohak Lee, Sang Yup |
|---|---|
| author |
Lee, Sang Jun Song, Hyohak Lee, Sang Yup |
| spellingShingle |
Lee, Sang Jun Song, Hyohak Lee, Sang Yup Applied and Environmental Microbiology Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
lee, sang jun |
| spelling |
Lee, Sang Jun Song, Hyohak Lee, Sang Yup 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.72.3.1939-1948.2006 <jats:title>ABSTRACT</jats:title> <jats:p> Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, <jats:italic>Mannheimia succiniciproducens</jats:italic> , gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation. Among three different CO <jats:sub>2</jats:sub> -fixing metabolic reactions catalyzed by phosphoenolpyruvate (PEP) carboxykinase, PEP carboxylase, and malic enzyme, PEP carboxykinase was the most important for the anaerobic growth of <jats:italic>M. succiniciproducens</jats:italic> and succinic acid production. Oxaloacetate formed by carboxylation of PEP was found to be converted to succinic acid by three sequential reactions catalyzed by malate dehydrogenase, fumarase, and fumarate reductase. Major metabolic pathways leading to by-product formation were successfully removed by disrupting the <jats:italic>ldhA</jats:italic> , <jats:italic>pflB</jats:italic> , <jats:italic>pta</jats:italic> , and <jats:italic>ackA</jats:italic> genes. This metabolically engineered LPK7 strain was able to produce 13.4 g/liter of succinic acid from 20 g/liter glucose with little or no formation of acetic, formic, and lactic acids, resulting in a succinic acid yield of 0.97 mol succinic acid per mol glucose. Fed-batch culture of <jats:italic>M. succiniciproducens</jats:italic> LPK7 with intermittent glucose feeding allowed the production of 52.4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid. </jats:p> Genome-Based Metabolic Engineering of <i>Mannheimia succiniciproducens</i> for Succinic Acid Production Applied and Environmental Microbiology |
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| title |
Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_unstemmed |
Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_full |
Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_fullStr |
Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_full_unstemmed |
Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_short |
Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_sort |
genome-based metabolic engineering of
<i>mannheimia succiniciproducens</i>
for succinic acid production |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.72.3.1939-1948.2006 |
| publishDate |
2006 |
| physical |
1939-1948 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium,
<jats:italic>Mannheimia succiniciproducens</jats:italic>
, gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation. Among three different CO
<jats:sub>2</jats:sub>
-fixing metabolic reactions catalyzed by phosphoenolpyruvate (PEP) carboxykinase, PEP carboxylase, and malic enzyme, PEP carboxykinase was the most important for the anaerobic growth of
<jats:italic>M. succiniciproducens</jats:italic>
and succinic acid production. Oxaloacetate formed by carboxylation of PEP was found to be converted to succinic acid by three sequential reactions catalyzed by malate dehydrogenase, fumarase, and fumarate reductase. Major metabolic pathways leading to by-product formation were successfully removed by disrupting the
<jats:italic>ldhA</jats:italic>
,
<jats:italic>pflB</jats:italic>
,
<jats:italic>pta</jats:italic>
, and
<jats:italic>ackA</jats:italic>
genes. This metabolically engineered LPK7 strain was able to produce 13.4 g/liter of succinic acid from 20 g/liter glucose with little or no formation of acetic, formic, and lactic acids, resulting in a succinic acid yield of 0.97 mol succinic acid per mol glucose. Fed-batch culture of
<jats:italic>M. succiniciproducens</jats:italic>
LPK7 with intermittent glucose feeding allowed the production of 52.4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid.
</jats:p> |
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| author | Lee, Sang Jun, Song, Hyohak, Lee, Sang Yup |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, <jats:italic>Mannheimia succiniciproducens</jats:italic> , gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation. Among three different CO <jats:sub>2</jats:sub> -fixing metabolic reactions catalyzed by phosphoenolpyruvate (PEP) carboxykinase, PEP carboxylase, and malic enzyme, PEP carboxykinase was the most important for the anaerobic growth of <jats:italic>M. succiniciproducens</jats:italic> and succinic acid production. Oxaloacetate formed by carboxylation of PEP was found to be converted to succinic acid by three sequential reactions catalyzed by malate dehydrogenase, fumarase, and fumarate reductase. Major metabolic pathways leading to by-product formation were successfully removed by disrupting the <jats:italic>ldhA</jats:italic> , <jats:italic>pflB</jats:italic> , <jats:italic>pta</jats:italic> , and <jats:italic>ackA</jats:italic> genes. This metabolically engineered LPK7 strain was able to produce 13.4 g/liter of succinic acid from 20 g/liter glucose with little or no formation of acetic, formic, and lactic acids, resulting in a succinic acid yield of 0.97 mol succinic acid per mol glucose. Fed-batch culture of <jats:italic>M. succiniciproducens</jats:italic> LPK7 with intermittent glucose feeding allowed the production of 52.4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid. </jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2006 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| physical | 1939-1948 |
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| spelling | Lee, Sang Jun Song, Hyohak Lee, Sang Yup 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.72.3.1939-1948.2006 <jats:title>ABSTRACT</jats:title> <jats:p> Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, <jats:italic>Mannheimia succiniciproducens</jats:italic> , gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation. Among three different CO <jats:sub>2</jats:sub> -fixing metabolic reactions catalyzed by phosphoenolpyruvate (PEP) carboxykinase, PEP carboxylase, and malic enzyme, PEP carboxykinase was the most important for the anaerobic growth of <jats:italic>M. succiniciproducens</jats:italic> and succinic acid production. Oxaloacetate formed by carboxylation of PEP was found to be converted to succinic acid by three sequential reactions catalyzed by malate dehydrogenase, fumarase, and fumarate reductase. Major metabolic pathways leading to by-product formation were successfully removed by disrupting the <jats:italic>ldhA</jats:italic> , <jats:italic>pflB</jats:italic> , <jats:italic>pta</jats:italic> , and <jats:italic>ackA</jats:italic> genes. This metabolically engineered LPK7 strain was able to produce 13.4 g/liter of succinic acid from 20 g/liter glucose with little or no formation of acetic, formic, and lactic acids, resulting in a succinic acid yield of 0.97 mol succinic acid per mol glucose. Fed-batch culture of <jats:italic>M. succiniciproducens</jats:italic> LPK7 with intermittent glucose feeding allowed the production of 52.4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid. </jats:p> Genome-Based Metabolic Engineering of <i>Mannheimia succiniciproducens</i> for Succinic Acid Production Applied and Environmental Microbiology |
| spellingShingle | Lee, Sang Jun, Song, Hyohak, Lee, Sang Yup, Applied and Environmental Microbiology, Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_full | Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_fullStr | Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_full_unstemmed | Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_short | Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| title_sort | genome-based metabolic engineering of <i>mannheimia succiniciproducens</i> for succinic acid production |
| title_unstemmed | Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.72.3.1939-1948.2006 |