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Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome...
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Applied and Environmental Microbiology, 69, 2003, 8, S. 4737-4742 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Choi, Jong Hyun Lee, Sang Jun Lee, Seok Jae Lee, Sang Yup Choi, Jong Hyun Lee, Sang Jun Lee, Seok Jae Lee, Sang Yup |
|---|---|
| author |
Choi, Jong Hyun Lee, Sang Jun Lee, Seok Jae Lee, Sang Yup |
| spellingShingle |
Choi, Jong Hyun Lee, Sang Jun Lee, Seok Jae Lee, Sang Yup Applied and Environmental Microbiology Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
choi, jong hyun |
| spelling |
Choi, Jong Hyun Lee, Sang Jun Lee, Seok Jae Lee, Sang Yup 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.8.4737-4742.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The transcriptome profiles of recombinant <jats:italic>Escherichia coli</jats:italic> producing human insulin-like growth factor I fusion protein (IGF-I <jats:sub>f</jats:sub> ) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. The expression levels of 529 genes were significantly altered after induction. About 200 genes were significantly down-regulated during the production of IGF-I <jats:sub>f</jats:sub> after induction. Among these down-regulated genes, we rationally selected and coexpressed in <jats:italic>E</jats:italic> . <jats:italic>coli</jats:italic> producing IGF-I <jats:sub>f</jats:sub> the <jats:italic>prsA</jats:italic> gene (encoding a phosphoribosyl pyrophosphate synthetase) and the <jats:italic>glpF</jats:italic> gene (encoding a glycerol transporter), which are involved in an early key step in the biosynthetic pathway of nucleotides and amino acids (Trp and His) and the first step in glycerol utilization, respectively. As a result, the production of IGF-I <jats:sub>f</jats:sub> could be increased from 1.8 ± 0.13 (± standard deviation) to 4.3 ± 0.24 g/liter. The volumetric productivity was also increased from 0.36 ± 0.027 to 0.82 ± 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance. </jats:p> Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in <i>Escherichia coli</i> by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling Applied and Environmental Microbiology |
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10.1128/aem.69.8.4737-4742.2003 |
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| title |
Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_unstemmed |
Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_full |
Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_fullStr |
Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_full_unstemmed |
Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_short |
Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_sort |
enhanced production of insulin-like growth factor i fusion protein in
<i>escherichia coli</i>
by coexpression of the down-regulated genes identified by transcriptome profiling |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.69.8.4737-4742.2003 |
| publishDate |
2003 |
| physical |
4737-4742 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The transcriptome profiles of recombinant
<jats:italic>Escherichia coli</jats:italic>
producing human insulin-like growth factor I fusion protein (IGF-I
<jats:sub>f</jats:sub>
) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. The expression levels of 529 genes were significantly altered after induction. About 200 genes were significantly down-regulated during the production of IGF-I
<jats:sub>f</jats:sub>
after induction. Among these down-regulated genes, we rationally selected and coexpressed in
<jats:italic>E</jats:italic>
.
<jats:italic>coli</jats:italic>
producing IGF-I
<jats:sub>f</jats:sub>
the
<jats:italic>prsA</jats:italic>
gene (encoding a phosphoribosyl pyrophosphate synthetase) and the
<jats:italic>glpF</jats:italic>
gene (encoding a glycerol transporter), which are involved in an early key step in the biosynthetic pathway of nucleotides and amino acids (Trp and His) and the first step in glycerol utilization, respectively. As a result, the production of IGF-I
<jats:sub>f</jats:sub>
could be increased from 1.8 ± 0.13 (± standard deviation) to 4.3 ± 0.24 g/liter. The volumetric productivity was also increased from 0.36 ± 0.027 to 0.82 ± 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance.
</jats:p> |
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| author | Choi, Jong Hyun, Lee, Sang Jun, Lee, Seok Jae, Lee, Sang Yup |
| author_facet | Choi, Jong Hyun, Lee, Sang Jun, Lee, Seok Jae, Lee, Sang Yup, Choi, Jong Hyun, Lee, Sang Jun, Lee, Seok Jae, Lee, Sang Yup |
| author_sort | choi, jong hyun |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The transcriptome profiles of recombinant <jats:italic>Escherichia coli</jats:italic> producing human insulin-like growth factor I fusion protein (IGF-I <jats:sub>f</jats:sub> ) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. The expression levels of 529 genes were significantly altered after induction. About 200 genes were significantly down-regulated during the production of IGF-I <jats:sub>f</jats:sub> after induction. Among these down-regulated genes, we rationally selected and coexpressed in <jats:italic>E</jats:italic> . <jats:italic>coli</jats:italic> producing IGF-I <jats:sub>f</jats:sub> the <jats:italic>prsA</jats:italic> gene (encoding a phosphoribosyl pyrophosphate synthetase) and the <jats:italic>glpF</jats:italic> gene (encoding a glycerol transporter), which are involved in an early key step in the biosynthetic pathway of nucleotides and amino acids (Trp and His) and the first step in glycerol utilization, respectively. As a result, the production of IGF-I <jats:sub>f</jats:sub> could be increased from 1.8 ± 0.13 (± standard deviation) to 4.3 ± 0.24 g/liter. The volumetric productivity was also increased from 0.36 ± 0.027 to 0.82 ± 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance. </jats:p> |
| doi_str_mv | 10.1128/aem.69.8.4737-4742.2003 |
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| imprint | American Society for Microbiology, 2003 |
| imprint_str_mv | American Society for Microbiology, 2003 |
| institution | DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0099-2240, 1098-5336 |
| issn_str_mv | 0099-2240, 1098-5336 |
| language | English |
| last_indexed | 2024-03-01T15:15:05.639Z |
| match_str | choi2003enhancedproductionofinsulinlikegrowthfactorifusionproteininescherichiacolibycoexpressionofthedownregulatedgenesidentifiedbytranscriptomeprofiling |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 4737-4742 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Applied and Environmental Microbiology |
| source_id | 49 |
| spelling | Choi, Jong Hyun Lee, Sang Jun Lee, Seok Jae Lee, Sang Yup 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.8.4737-4742.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The transcriptome profiles of recombinant <jats:italic>Escherichia coli</jats:italic> producing human insulin-like growth factor I fusion protein (IGF-I <jats:sub>f</jats:sub> ) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. The expression levels of 529 genes were significantly altered after induction. About 200 genes were significantly down-regulated during the production of IGF-I <jats:sub>f</jats:sub> after induction. Among these down-regulated genes, we rationally selected and coexpressed in <jats:italic>E</jats:italic> . <jats:italic>coli</jats:italic> producing IGF-I <jats:sub>f</jats:sub> the <jats:italic>prsA</jats:italic> gene (encoding a phosphoribosyl pyrophosphate synthetase) and the <jats:italic>glpF</jats:italic> gene (encoding a glycerol transporter), which are involved in an early key step in the biosynthetic pathway of nucleotides and amino acids (Trp and His) and the first step in glycerol utilization, respectively. As a result, the production of IGF-I <jats:sub>f</jats:sub> could be increased from 1.8 ± 0.13 (± standard deviation) to 4.3 ± 0.24 g/liter. The volumetric productivity was also increased from 0.36 ± 0.027 to 0.82 ± 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance. </jats:p> Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in <i>Escherichia coli</i> by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling Applied and Environmental Microbiology |
| spellingShingle | Choi, Jong Hyun, Lee, Sang Jun, Lee, Seok Jae, Lee, Sang Yup, Applied and Environmental Microbiology, Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_full | Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_fullStr | Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_full_unstemmed | Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_short | Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| title_sort | enhanced production of insulin-like growth factor i fusion protein in <i>escherichia coli</i> by coexpression of the down-regulated genes identified by transcriptome profiling |
| title_unstemmed | Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.69.8.4737-4742.2003 |