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Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the...
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Applied and Environmental Microbiology, 69, 2003, 12, S. 7545-7548 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Nielsen, D. S. Møller, P. L. Rosenfeldt, V. Pærregaard, A. Michaelsen, K. F. Jakobsen, M. Nielsen, D. S. Møller, P. L. Rosenfeldt, V. Pærregaard, A. Michaelsen, K. F. Jakobsen, M. |
|---|---|
| author |
Nielsen, D. S. Møller, P. L. Rosenfeldt, V. Pærregaard, A. Michaelsen, K. F. Jakobsen, M. |
| spellingShingle |
Nielsen, D. S. Møller, P. L. Rosenfeldt, V. Pærregaard, A. Michaelsen, K. F. Jakobsen, M. Applied and Environmental Microbiology Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
nielsen, d. s. |
| spelling |
Nielsen, D. S. Møller, P. L. Rosenfeldt, V. Pærregaard, A. Michaelsen, K. F. Jakobsen, M. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.12.7545-7548.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, <jats:italic>Lactobacillus</jats:italic> group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with <jats:italic>Lactobacillus</jats:italic> group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site. </jats:p> Case Study of the Distribution of Mucosa-Associated <i>Bifidobacterium</i> Species, <i>Lactobacillus</i> Species, andOther Lactic Acid Bacteria in the HumanColon Applied and Environmental Microbiology |
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10.1128/aem.69.12.7545-7548.2003 |
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Online Free |
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Geographie Biologie Technik Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft |
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American Society for Microbiology, 2003 |
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American Society for Microbiology, 2003 |
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0099-2240 1098-5336 |
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Applied and Environmental Microbiology |
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49 |
| title |
Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_unstemmed |
Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_full |
Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_fullStr |
Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_full_unstemmed |
Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_short |
Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_sort |
case study of the distribution of mucosa-associated
<i>bifidobacterium</i>
species,
<i>lactobacillus</i>
species, andother lactic acid bacteria in the humancolon |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.69.12.7545-7548.2003 |
| publishDate |
2003 |
| physical |
7545-7548 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The
distribution of mucosa-associated bacteria, bifidobacteria and
lactobacilli and closely related lactic acid bacteria, in biopsy
samples from the ascending, transverse, and descending parts of the
colon from four individuals was investigated by denaturing gradient gel
electrophoresis (DGGE). Bifidobacterial genus-specific,
<jats:italic>Lactobacillus</jats:italic>
group-specific, and universal bacterial primers
were used in a nested PCR approach to amplify a fragment of the 16S
rRNA gene. DGGE profiles of the bifidobacterial community were
relatively simple, with one or two amplicons detected at most sampling
sites in the colon. DGGE profiles obtained with
<jats:italic>Lactobacillus</jats:italic>
group-specific primers were complex and varied with host and sampling
site in the colon. The overall bacterial community varied with host but
not sampling
site.
</jats:p> |
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| author | Nielsen, D. S., Møller, P. L., Rosenfeldt, V., Pærregaard, A., Michaelsen, K. F., Jakobsen, M. |
| author_facet | Nielsen, D. S., Møller, P. L., Rosenfeldt, V., Pærregaard, A., Michaelsen, K. F., Jakobsen, M., Nielsen, D. S., Møller, P. L., Rosenfeldt, V., Pærregaard, A., Michaelsen, K. F., Jakobsen, M. |
| author_sort | nielsen, d. s. |
| container_issue | 12 |
| container_start_page | 7545 |
| container_title | Applied and Environmental Microbiology |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, <jats:italic>Lactobacillus</jats:italic> group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with <jats:italic>Lactobacillus</jats:italic> group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site. </jats:p> |
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| imprint | American Society for Microbiology, 2003 |
| imprint_str_mv | American Society for Microbiology, 2003 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| issn_str_mv | 0099-2240, 1098-5336 |
| language | English |
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| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 7545-7548 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | American Society for Microbiology |
| record_format | ai |
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| series | Applied and Environmental Microbiology |
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| spelling | Nielsen, D. S. Møller, P. L. Rosenfeldt, V. Pærregaard, A. Michaelsen, K. F. Jakobsen, M. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.12.7545-7548.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, <jats:italic>Lactobacillus</jats:italic> group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with <jats:italic>Lactobacillus</jats:italic> group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site. </jats:p> Case Study of the Distribution of Mucosa-Associated <i>Bifidobacterium</i> Species, <i>Lactobacillus</i> Species, andOther Lactic Acid Bacteria in the HumanColon Applied and Environmental Microbiology |
| spellingShingle | Nielsen, D. S., Møller, P. L., Rosenfeldt, V., Pærregaard, A., Michaelsen, K. F., Jakobsen, M., Applied and Environmental Microbiology, Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_full | Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_fullStr | Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_full_unstemmed | Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_short | Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| title_sort | case study of the distribution of mucosa-associated <i>bifidobacterium</i> species, <i>lactobacillus</i> species, andother lactic acid bacteria in the humancolon |
| title_unstemmed | Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.69.12.7545-7548.2003 |