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Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Applied and Environmental Microbiology, 68, 2002, 1, S. 372-378 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Egan, Suhelen James, Sally Kjelleberg, Staffan Egan, Suhelen James, Sally Kjelleberg, Staffan |
|---|---|
| author |
Egan, Suhelen James, Sally Kjelleberg, Staffan |
| spellingShingle |
Egan, Suhelen James, Sally Kjelleberg, Staffan Applied and Environmental Microbiology Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
egan, suhelen |
| spelling |
Egan, Suhelen James, Sally Kjelleberg, Staffan 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.68.1.372-378.2002 <jats:title>ABSTRACT</jats:title> <jats:p> The dark green pigmented marine bacterium <jats:italic>Pseudoalteromonas tunicata</jats:italic> colonizes living surfaces and produces a range of extracellular compounds that inhibit common fouling organisms, including marine invertebrate larvae, algae, bacteria, and fungi. We have observed a positive correlation between the antifouling activity of <jats:italic>P. tunicata</jats:italic> strain D2 and the expression of pigmentation. To address the hypothesis that pigmentation and antifouling may be jointly regulated in this organism and to begin to identify potential regulatory elements, we used transposon mutagenesis to generate a strain of <jats:italic>P. tunicata</jats:italic> deficient in antifouling activity. The data presented here describe the phenotypic and molecular characterization of a nonpigmented transposon mutant strain of <jats:italic>P. tunicata</jats:italic> (D2W2). Analyses of the antifouling capabilities of D2W2 demonstrate that this strain is deficient in the ability to inhibit each of the target fouling organisms. Genetic analysis of D2W2 identified a gene, designated <jats:italic>wmpR</jats:italic> (white mutant phenotype), with high sequence similarity to transcriptional regulators ToxR from <jats:italic>Vibrio cholerae</jats:italic> and CadC from <jats:italic>Escherichia coli</jats:italic> . Two-dimensional polyacrylamide gel electrophoresis analysis revealed that WmpR is essential for the expression of a significant subset of stationary-phase-induced proteins likely to be important for the synthesis of fouling inhibitors. The identification of a gene involved in the regulation of expression of antifouling phenotypes will contribute to the understanding of the interactions between bacteria and other surface-colonizing organisms in the marine environment. </jats:p> Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in <i>Pseudoalteromonas tunicata</i> Applied and Environmental Microbiology |
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| title |
Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_unstemmed |
Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_full |
Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_fullStr |
Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_full_unstemmed |
Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_short |
Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_sort |
identification and characterization of a putative transcriptional regulator controlling the expression of fouling inhibitors in
<i>pseudoalteromonas tunicata</i> |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.68.1.372-378.2002 |
| publishDate |
2002 |
| physical |
372-378 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The dark green pigmented marine bacterium
<jats:italic>Pseudoalteromonas tunicata</jats:italic>
colonizes living surfaces and produces a range of extracellular compounds that inhibit common fouling organisms, including marine invertebrate larvae, algae, bacteria, and fungi. We have observed a positive correlation between the antifouling activity of
<jats:italic>P. tunicata</jats:italic>
strain D2 and the expression of pigmentation. To address the hypothesis that pigmentation and antifouling may be jointly regulated in this organism and to begin to identify potential regulatory elements, we used transposon mutagenesis to generate a strain of
<jats:italic>P. tunicata</jats:italic>
deficient in antifouling activity. The data presented here describe the phenotypic and molecular characterization of a nonpigmented transposon mutant strain of
<jats:italic>P. tunicata</jats:italic>
(D2W2). Analyses of the antifouling capabilities of D2W2 demonstrate that this strain is deficient in the ability to inhibit each of the target fouling organisms. Genetic analysis of D2W2 identified a gene, designated
<jats:italic>wmpR</jats:italic>
(white mutant phenotype), with high sequence similarity to transcriptional regulators ToxR from
<jats:italic>Vibrio cholerae</jats:italic>
and CadC from
<jats:italic>Escherichia coli</jats:italic>
. Two-dimensional polyacrylamide gel electrophoresis analysis revealed that WmpR is essential for the expression of a significant subset of stationary-phase-induced proteins likely to be important for the synthesis of fouling inhibitors. The identification of a gene involved in the regulation of expression of antifouling phenotypes will contribute to the understanding of the interactions between bacteria and other surface-colonizing organisms in the marine environment.
</jats:p> |
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| author | Egan, Suhelen, James, Sally, Kjelleberg, Staffan |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The dark green pigmented marine bacterium <jats:italic>Pseudoalteromonas tunicata</jats:italic> colonizes living surfaces and produces a range of extracellular compounds that inhibit common fouling organisms, including marine invertebrate larvae, algae, bacteria, and fungi. We have observed a positive correlation between the antifouling activity of <jats:italic>P. tunicata</jats:italic> strain D2 and the expression of pigmentation. To address the hypothesis that pigmentation and antifouling may be jointly regulated in this organism and to begin to identify potential regulatory elements, we used transposon mutagenesis to generate a strain of <jats:italic>P. tunicata</jats:italic> deficient in antifouling activity. The data presented here describe the phenotypic and molecular characterization of a nonpigmented transposon mutant strain of <jats:italic>P. tunicata</jats:italic> (D2W2). Analyses of the antifouling capabilities of D2W2 demonstrate that this strain is deficient in the ability to inhibit each of the target fouling organisms. Genetic analysis of D2W2 identified a gene, designated <jats:italic>wmpR</jats:italic> (white mutant phenotype), with high sequence similarity to transcriptional regulators ToxR from <jats:italic>Vibrio cholerae</jats:italic> and CadC from <jats:italic>Escherichia coli</jats:italic> . Two-dimensional polyacrylamide gel electrophoresis analysis revealed that WmpR is essential for the expression of a significant subset of stationary-phase-induced proteins likely to be important for the synthesis of fouling inhibitors. The identification of a gene involved in the regulation of expression of antifouling phenotypes will contribute to the understanding of the interactions between bacteria and other surface-colonizing organisms in the marine environment. </jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2002 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| spelling | Egan, Suhelen James, Sally Kjelleberg, Staffan 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.68.1.372-378.2002 <jats:title>ABSTRACT</jats:title> <jats:p> The dark green pigmented marine bacterium <jats:italic>Pseudoalteromonas tunicata</jats:italic> colonizes living surfaces and produces a range of extracellular compounds that inhibit common fouling organisms, including marine invertebrate larvae, algae, bacteria, and fungi. We have observed a positive correlation between the antifouling activity of <jats:italic>P. tunicata</jats:italic> strain D2 and the expression of pigmentation. To address the hypothesis that pigmentation and antifouling may be jointly regulated in this organism and to begin to identify potential regulatory elements, we used transposon mutagenesis to generate a strain of <jats:italic>P. tunicata</jats:italic> deficient in antifouling activity. The data presented here describe the phenotypic and molecular characterization of a nonpigmented transposon mutant strain of <jats:italic>P. tunicata</jats:italic> (D2W2). Analyses of the antifouling capabilities of D2W2 demonstrate that this strain is deficient in the ability to inhibit each of the target fouling organisms. Genetic analysis of D2W2 identified a gene, designated <jats:italic>wmpR</jats:italic> (white mutant phenotype), with high sequence similarity to transcriptional regulators ToxR from <jats:italic>Vibrio cholerae</jats:italic> and CadC from <jats:italic>Escherichia coli</jats:italic> . Two-dimensional polyacrylamide gel electrophoresis analysis revealed that WmpR is essential for the expression of a significant subset of stationary-phase-induced proteins likely to be important for the synthesis of fouling inhibitors. The identification of a gene involved in the regulation of expression of antifouling phenotypes will contribute to the understanding of the interactions between bacteria and other surface-colonizing organisms in the marine environment. </jats:p> Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in <i>Pseudoalteromonas tunicata</i> Applied and Environmental Microbiology |
| spellingShingle | Egan, Suhelen, James, Sally, Kjelleberg, Staffan, Applied and Environmental Microbiology, Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_full | Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_fullStr | Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_full_unstemmed | Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_short | Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| title_sort | identification and characterization of a putative transcriptional regulator controlling the expression of fouling inhibitors in <i>pseudoalteromonas tunicata</i> |
| title_unstemmed | Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.68.1.372-378.2002 |