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Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Applied and Environmental Microbiology, 67, 2001, 12, S. 5721-5728 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Churchill, Alice C. L. Dunkle, Larry D. Silbert, Walter Kennedy, Kevin J. Macko, Vlado Churchill, Alice C. L. Dunkle, Larry D. Silbert, Walter Kennedy, Kevin J. Macko, Vlado |
|---|---|
| author |
Churchill, Alice C. L. Dunkle, Larry D. Silbert, Walter Kennedy, Kevin J. Macko, Vlado |
| spellingShingle |
Churchill, Alice C. L. Dunkle, Larry D. Silbert, Walter Kennedy, Kevin J. Macko, Vlado Applied and Environmental Microbiology Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
churchill, alice c. l. |
| spelling |
Churchill, Alice C. L. Dunkle, Larry D. Silbert, Walter Kennedy, Kevin J. Macko, Vlado 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.67.12.5721-5728.2001 <jats:title>ABSTRACT</jats:title> <jats:p> Pathogenic strains of the soilborne fungus <jats:italic>Periconia circinata</jats:italic> produce peritoxins with host-selective toxicity against susceptible genotypes of sorghum. The peritoxins are low-molecular-weight, hybrid molecules consisting of a peptide and a chlorinated polyketide. Culture fluids from pathogenic, toxin-producing (Tox <jats:sup>+</jats:sup> ) and nonpathogenic, non-toxin-producing (Tox <jats:sup>−</jats:sup> ) strains were analyzed directly by gradient high-performance liquid chromatography (HPLC) with photodiode array detection and HPLC-mass spectrometry to detect intermediates and final products of the biosynthetic pathway. This approach allowed us to compare the metabolite profiles of Tox <jats:sup>+</jats:sup> and Tox <jats:sup>−</jats:sup> strains. Peritoxins A and B and the biologically inactive intermediates, <jats:italic>N</jats:italic> -3-( <jats:italic>E</jats:italic> -pentenyl)-glutaroyl-aspartate, circinatin, and 7-chlorocircinatin, were detected only in culture fluids of the Tox <jats:sup>+</jats:sup> strains. The latter two compounds were produced consistently by Tox <jats:sup>+</jats:sup> strains regardless of the amount of peritoxins produced under various culture conditions. In summary, none of the known peritoxin-related metabolites were detected in Tox <jats:sup>−</jats:sup> strains, which suggests that these strains may lack one or more functional genes required for peritoxin biosynthesis. </jats:p> Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus <i>Periconia circinata</i> Applied and Environmental Microbiology |
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10.1128/aem.67.12.5721-5728.2001 |
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2001 |
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| title |
Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_unstemmed |
Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_full |
Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_fullStr |
Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_full_unstemmed |
Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_short |
Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_sort |
differential synthesis of peritoxins and precursors by pathogenic strains of the fungus
<i>periconia circinata</i> |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.67.12.5721-5728.2001 |
| publishDate |
2001 |
| physical |
5721-5728 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Pathogenic strains of the soilborne fungus
<jats:italic>Periconia circinata</jats:italic>
produce peritoxins with host-selective toxicity against susceptible genotypes of sorghum. The peritoxins are low-molecular-weight, hybrid molecules consisting of a peptide and a chlorinated polyketide. Culture fluids from pathogenic, toxin-producing (Tox
<jats:sup>+</jats:sup>
) and nonpathogenic, non-toxin-producing (Tox
<jats:sup>−</jats:sup>
) strains were analyzed directly by gradient high-performance liquid chromatography (HPLC) with photodiode array detection and HPLC-mass spectrometry to detect intermediates and final products of the biosynthetic pathway. This approach allowed us to compare the metabolite profiles of Tox
<jats:sup>+</jats:sup>
and Tox
<jats:sup>−</jats:sup>
strains. Peritoxins A and B and the biologically inactive intermediates,
<jats:italic>N</jats:italic>
-3-(
<jats:italic>E</jats:italic>
-pentenyl)-glutaroyl-aspartate, circinatin, and 7-chlorocircinatin, were detected only in culture fluids of the Tox
<jats:sup>+</jats:sup>
strains. The latter two compounds were produced consistently by Tox
<jats:sup>+</jats:sup>
strains regardless of the amount of peritoxins produced under various culture conditions. In summary, none of the known peritoxin-related metabolites were detected in Tox
<jats:sup>−</jats:sup>
strains, which suggests that these strains may lack one or more functional genes required for peritoxin biosynthesis.
</jats:p> |
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| author | Churchill, Alice C. L., Dunkle, Larry D., Silbert, Walter, Kennedy, Kevin J., Macko, Vlado |
| author_facet | Churchill, Alice C. L., Dunkle, Larry D., Silbert, Walter, Kennedy, Kevin J., Macko, Vlado, Churchill, Alice C. L., Dunkle, Larry D., Silbert, Walter, Kennedy, Kevin J., Macko, Vlado |
| author_sort | churchill, alice c. l. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Pathogenic strains of the soilborne fungus <jats:italic>Periconia circinata</jats:italic> produce peritoxins with host-selective toxicity against susceptible genotypes of sorghum. The peritoxins are low-molecular-weight, hybrid molecules consisting of a peptide and a chlorinated polyketide. Culture fluids from pathogenic, toxin-producing (Tox <jats:sup>+</jats:sup> ) and nonpathogenic, non-toxin-producing (Tox <jats:sup>−</jats:sup> ) strains were analyzed directly by gradient high-performance liquid chromatography (HPLC) with photodiode array detection and HPLC-mass spectrometry to detect intermediates and final products of the biosynthetic pathway. This approach allowed us to compare the metabolite profiles of Tox <jats:sup>+</jats:sup> and Tox <jats:sup>−</jats:sup> strains. Peritoxins A and B and the biologically inactive intermediates, <jats:italic>N</jats:italic> -3-( <jats:italic>E</jats:italic> -pentenyl)-glutaroyl-aspartate, circinatin, and 7-chlorocircinatin, were detected only in culture fluids of the Tox <jats:sup>+</jats:sup> strains. The latter two compounds were produced consistently by Tox <jats:sup>+</jats:sup> strains regardless of the amount of peritoxins produced under various culture conditions. In summary, none of the known peritoxin-related metabolites were detected in Tox <jats:sup>−</jats:sup> strains, which suggests that these strains may lack one or more functional genes required for peritoxin biosynthesis. </jats:p> |
| doi_str_mv | 10.1128/aem.67.12.5721-5728.2001 |
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| imprint | American Society for Microbiology, 2001 |
| imprint_str_mv | American Society for Microbiology, 2001 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| issn_str_mv | 1098-5336, 0099-2240 |
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| physical | 5721-5728 |
| publishDate | 2001 |
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| publisher | American Society for Microbiology |
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| recordtype | ai |
| series | Applied and Environmental Microbiology |
| source_id | 49 |
| spelling | Churchill, Alice C. L. Dunkle, Larry D. Silbert, Walter Kennedy, Kevin J. Macko, Vlado 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.67.12.5721-5728.2001 <jats:title>ABSTRACT</jats:title> <jats:p> Pathogenic strains of the soilborne fungus <jats:italic>Periconia circinata</jats:italic> produce peritoxins with host-selective toxicity against susceptible genotypes of sorghum. The peritoxins are low-molecular-weight, hybrid molecules consisting of a peptide and a chlorinated polyketide. Culture fluids from pathogenic, toxin-producing (Tox <jats:sup>+</jats:sup> ) and nonpathogenic, non-toxin-producing (Tox <jats:sup>−</jats:sup> ) strains were analyzed directly by gradient high-performance liquid chromatography (HPLC) with photodiode array detection and HPLC-mass spectrometry to detect intermediates and final products of the biosynthetic pathway. This approach allowed us to compare the metabolite profiles of Tox <jats:sup>+</jats:sup> and Tox <jats:sup>−</jats:sup> strains. Peritoxins A and B and the biologically inactive intermediates, <jats:italic>N</jats:italic> -3-( <jats:italic>E</jats:italic> -pentenyl)-glutaroyl-aspartate, circinatin, and 7-chlorocircinatin, were detected only in culture fluids of the Tox <jats:sup>+</jats:sup> strains. The latter two compounds were produced consistently by Tox <jats:sup>+</jats:sup> strains regardless of the amount of peritoxins produced under various culture conditions. In summary, none of the known peritoxin-related metabolites were detected in Tox <jats:sup>−</jats:sup> strains, which suggests that these strains may lack one or more functional genes required for peritoxin biosynthesis. </jats:p> Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus <i>Periconia circinata</i> Applied and Environmental Microbiology |
| spellingShingle | Churchill, Alice C. L., Dunkle, Larry D., Silbert, Walter, Kennedy, Kevin J., Macko, Vlado, Applied and Environmental Microbiology, Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_full | Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_fullStr | Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_full_unstemmed | Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_short | Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| title_sort | differential synthesis of peritoxins and precursors by pathogenic strains of the fungus <i>periconia circinata</i> |
| title_unstemmed | Differential Synthesis of Peritoxins and Precursors by Pathogenic Strains of the Fungus Periconia circinata |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.67.12.5721-5728.2001 |