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Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Applied and Environmental Microbiology, 78, 2012, 5, S. 1513-1522 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Schumann, Sara Alpert, Carl Engst, Wolfram Loh, Gunnar Blaut, Michael Schumann, Sara Alpert, Carl Engst, Wolfram Loh, Gunnar Blaut, Michael |
|---|---|
| author |
Schumann, Sara Alpert, Carl Engst, Wolfram Loh, Gunnar Blaut, Michael |
| spellingShingle |
Schumann, Sara Alpert, Carl Engst, Wolfram Loh, Gunnar Blaut, Michael Applied and Environmental Microbiology Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
schumann, sara |
| spelling |
Schumann, Sara Alpert, Carl Engst, Wolfram Loh, Gunnar Blaut, Michael 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.07340-11 <jats:title>ABSTRACT</jats:title> <jats:p> To identify <jats:named-content content-type="genus-species">Escherichia coli</jats:named-content> proteins involved in adaptation to intestinal inflammation, mice were monoassociated with the colitogenic <jats:named-content content-type="genus-species">E. coli</jats:named-content> strain UNC or with the probiotic <jats:named-content content-type="genus-species">E. coli</jats:named-content> strain Nissle. Intestinal inflammation was induced by treating the mice with 3.5% dextran sodium sulfate (DSS). Differentially expressed proteins in <jats:named-content content-type="genus-species">E. coli</jats:named-content> strains collected from cecal contents were identified by 2-dimensional difference gel electrophoresis. In both strains, acute inflammation led to the downregulation of pathways involved in carbohydrate breakdown and energy generation. Accordingly, DSS-treated mice had lower concentrations of bacterial fermentation products in their cecal contents than control mice. Differentially expressed proteins also included the Fe-S cluster repair protein NfuA, the tryptophanase TnaA, and the uncharacterized protein YggE. NfuA expression was 3-fold higher in <jats:named-content content-type="genus-species">E. coli</jats:named-content> strains from DSS-treated than from control mice. Reporter experiments confirmed the induction of <jats:italic>nfuA</jats:italic> in response to iron deprivation, mimicking Fe-S cluster destruction by inflammation. YggE expression, which has been reported to reduce the intracellular level of reactive oxygen species, was 4- to 8-fold higher in <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle than in <jats:named-content content-type="genus-species">E. coli</jats:named-content> UNC. This was confirmed by <jats:italic>in vitro</jats:italic> reporter gene assays indicating that Nissle is better equipped to cope with oxidative stress than UNC. Nissle isolated from DSS-treated and control mice had TnaA levels 4- to 7-fold-higher than those of UNC. Levels of indole resulting from the TnaA reaction were higher in control animals associated with <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle. Because of its anti-inflammatory effect, indole is hypothesized to be involved in the extension of the remission phase in ulcerative colitis described for <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle. </jats:p> Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model Applied and Environmental Microbiology |
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10.1128/aem.07340-11 |
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American Society for Microbiology, 2012 |
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American Society for Microbiology, 2012 |
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2012 |
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American Society for Microbiology |
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Applied and Environmental Microbiology |
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49 |
| title |
Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_unstemmed |
Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_full |
Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_fullStr |
Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_full_unstemmed |
Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_short |
Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_sort |
dextran sodium sulfate-induced inflammation alters the expression of proteins by intestinal escherichia coli strains in a gnotobiotic mouse model |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.07340-11 |
| publishDate |
2012 |
| physical |
1513-1522 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
To identify
<jats:named-content content-type="genus-species">Escherichia coli</jats:named-content>
proteins involved in adaptation to intestinal inflammation, mice were monoassociated with the colitogenic
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
strain UNC or with the probiotic
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
strain Nissle. Intestinal inflammation was induced by treating the mice with 3.5% dextran sodium sulfate (DSS). Differentially expressed proteins in
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
strains collected from cecal contents were identified by 2-dimensional difference gel electrophoresis. In both strains, acute inflammation led to the downregulation of pathways involved in carbohydrate breakdown and energy generation. Accordingly, DSS-treated mice had lower concentrations of bacterial fermentation products in their cecal contents than control mice. Differentially expressed proteins also included the Fe-S cluster repair protein NfuA, the tryptophanase TnaA, and the uncharacterized protein YggE. NfuA expression was 3-fold higher in
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
strains from DSS-treated than from control mice. Reporter experiments confirmed the induction of
<jats:italic>nfuA</jats:italic>
in response to iron deprivation, mimicking Fe-S cluster destruction by inflammation. YggE expression, which has been reported to reduce the intracellular level of reactive oxygen species, was 4- to 8-fold higher in
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
Nissle than in
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
UNC. This was confirmed by
<jats:italic>in vitro</jats:italic>
reporter gene assays indicating that Nissle is better equipped to cope with oxidative stress than UNC. Nissle isolated from DSS-treated and control mice had TnaA levels 4- to 7-fold-higher than those of UNC. Levels of indole resulting from the TnaA reaction were higher in control animals associated with
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
Nissle. Because of its anti-inflammatory effect, indole is hypothesized to be involved in the extension of the remission phase in ulcerative colitis described for
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
Nissle.
</jats:p> |
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| author | Schumann, Sara, Alpert, Carl, Engst, Wolfram, Loh, Gunnar, Blaut, Michael |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> To identify <jats:named-content content-type="genus-species">Escherichia coli</jats:named-content> proteins involved in adaptation to intestinal inflammation, mice were monoassociated with the colitogenic <jats:named-content content-type="genus-species">E. coli</jats:named-content> strain UNC or with the probiotic <jats:named-content content-type="genus-species">E. coli</jats:named-content> strain Nissle. Intestinal inflammation was induced by treating the mice with 3.5% dextran sodium sulfate (DSS). Differentially expressed proteins in <jats:named-content content-type="genus-species">E. coli</jats:named-content> strains collected from cecal contents were identified by 2-dimensional difference gel electrophoresis. In both strains, acute inflammation led to the downregulation of pathways involved in carbohydrate breakdown and energy generation. Accordingly, DSS-treated mice had lower concentrations of bacterial fermentation products in their cecal contents than control mice. Differentially expressed proteins also included the Fe-S cluster repair protein NfuA, the tryptophanase TnaA, and the uncharacterized protein YggE. NfuA expression was 3-fold higher in <jats:named-content content-type="genus-species">E. coli</jats:named-content> strains from DSS-treated than from control mice. Reporter experiments confirmed the induction of <jats:italic>nfuA</jats:italic> in response to iron deprivation, mimicking Fe-S cluster destruction by inflammation. YggE expression, which has been reported to reduce the intracellular level of reactive oxygen species, was 4- to 8-fold higher in <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle than in <jats:named-content content-type="genus-species">E. coli</jats:named-content> UNC. This was confirmed by <jats:italic>in vitro</jats:italic> reporter gene assays indicating that Nissle is better equipped to cope with oxidative stress than UNC. Nissle isolated from DSS-treated and control mice had TnaA levels 4- to 7-fold-higher than those of UNC. Levels of indole resulting from the TnaA reaction were higher in control animals associated with <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle. Because of its anti-inflammatory effect, indole is hypothesized to be involved in the extension of the remission phase in ulcerative colitis described for <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle. </jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2012 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4 |
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| physical | 1513-1522 |
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| spelling | Schumann, Sara Alpert, Carl Engst, Wolfram Loh, Gunnar Blaut, Michael 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.07340-11 <jats:title>ABSTRACT</jats:title> <jats:p> To identify <jats:named-content content-type="genus-species">Escherichia coli</jats:named-content> proteins involved in adaptation to intestinal inflammation, mice were monoassociated with the colitogenic <jats:named-content content-type="genus-species">E. coli</jats:named-content> strain UNC or with the probiotic <jats:named-content content-type="genus-species">E. coli</jats:named-content> strain Nissle. Intestinal inflammation was induced by treating the mice with 3.5% dextran sodium sulfate (DSS). Differentially expressed proteins in <jats:named-content content-type="genus-species">E. coli</jats:named-content> strains collected from cecal contents were identified by 2-dimensional difference gel electrophoresis. In both strains, acute inflammation led to the downregulation of pathways involved in carbohydrate breakdown and energy generation. Accordingly, DSS-treated mice had lower concentrations of bacterial fermentation products in their cecal contents than control mice. Differentially expressed proteins also included the Fe-S cluster repair protein NfuA, the tryptophanase TnaA, and the uncharacterized protein YggE. NfuA expression was 3-fold higher in <jats:named-content content-type="genus-species">E. coli</jats:named-content> strains from DSS-treated than from control mice. Reporter experiments confirmed the induction of <jats:italic>nfuA</jats:italic> in response to iron deprivation, mimicking Fe-S cluster destruction by inflammation. YggE expression, which has been reported to reduce the intracellular level of reactive oxygen species, was 4- to 8-fold higher in <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle than in <jats:named-content content-type="genus-species">E. coli</jats:named-content> UNC. This was confirmed by <jats:italic>in vitro</jats:italic> reporter gene assays indicating that Nissle is better equipped to cope with oxidative stress than UNC. Nissle isolated from DSS-treated and control mice had TnaA levels 4- to 7-fold-higher than those of UNC. Levels of indole resulting from the TnaA reaction were higher in control animals associated with <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle. Because of its anti-inflammatory effect, indole is hypothesized to be involved in the extension of the remission phase in ulcerative colitis described for <jats:named-content content-type="genus-species">E. coli</jats:named-content> Nissle. </jats:p> Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model Applied and Environmental Microbiology |
| spellingShingle | Schumann, Sara, Alpert, Carl, Engst, Wolfram, Loh, Gunnar, Blaut, Michael, Applied and Environmental Microbiology, Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_full | Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_fullStr | Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_full_unstemmed | Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_short | Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| title_sort | dextran sodium sulfate-induced inflammation alters the expression of proteins by intestinal escherichia coli strains in a gnotobiotic mouse model |
| title_unstemmed | Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.07340-11 |