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Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece...
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Applied and Environmental Microbiology, 80, 2014, 5, S. 1763-1769 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Sopun, Warangkana Tanaka, Yoshito Takabe, Teruhiro Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Sopun, Warangkana Tanaka, Yoshito Takabe, Teruhiro |
|---|---|
| author |
Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Sopun, Warangkana Tanaka, Yoshito Takabe, Teruhiro |
| spellingShingle |
Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Sopun, Warangkana Tanaka, Yoshito Takabe, Teruhiro Applied and Environmental Microbiology Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
waditee-sirisattha, rungaroon |
| spelling |
Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Sopun, Warangkana Tanaka, Yoshito Takabe, Teruhiro 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.03729-13 <jats:title>ABSTRACT</jats:title> <jats:p> Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium <jats:named-content content-type="genus-species">Aphanothece halophytica</jats:named-content> , which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes. <jats:italic>In vivo</jats:italic> analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The <jats:named-content content-type="genus-species">Aphanothece</jats:named-content> MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed <jats:named-content content-type="genus-species">Escherichia coli</jats:named-content> and <jats:named-content content-type="genus-species">Synechococcus</jats:named-content> strains expressing four putative <jats:named-content content-type="genus-species">Aphanothece</jats:named-content> MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed <jats:named-content content-type="genus-species">E. coli</jats:named-content> cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source. </jats:p> Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica Applied and Environmental Microbiology |
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10.1128/aem.03729-13 |
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American Society for Microbiology, 2014 |
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American Society for Microbiology, 2014 |
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0099-2240 1098-5336 |
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| title |
Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_unstemmed |
Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_full |
Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_fullStr |
Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_full_unstemmed |
Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_short |
Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_sort |
identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium aphanothece halophytica |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.03729-13 |
| publishDate |
2014 |
| physical |
1763-1769 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium
<jats:named-content content-type="genus-species">Aphanothece halophytica</jats:named-content>
, which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes.
<jats:italic>In vivo</jats:italic>
analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The
<jats:named-content content-type="genus-species">Aphanothece</jats:named-content>
MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed
<jats:named-content content-type="genus-species">Escherichia coli</jats:named-content>
and
<jats:named-content content-type="genus-species">Synechococcus</jats:named-content>
strains expressing four putative
<jats:named-content content-type="genus-species">Aphanothece</jats:named-content>
MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source.
</jats:p> |
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| author | Waditee-Sirisattha, Rungaroon, Kageyama, Hakuto, Sopun, Warangkana, Tanaka, Yoshito, Takabe, Teruhiro |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium <jats:named-content content-type="genus-species">Aphanothece halophytica</jats:named-content> , which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes. <jats:italic>In vivo</jats:italic> analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The <jats:named-content content-type="genus-species">Aphanothece</jats:named-content> MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed <jats:named-content content-type="genus-species">Escherichia coli</jats:named-content> and <jats:named-content content-type="genus-species">Synechococcus</jats:named-content> strains expressing four putative <jats:named-content content-type="genus-species">Aphanothece</jats:named-content> MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed <jats:named-content content-type="genus-species">E. coli</jats:named-content> cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source. </jats:p> |
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| spelling | Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Sopun, Warangkana Tanaka, Yoshito Takabe, Teruhiro 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.03729-13 <jats:title>ABSTRACT</jats:title> <jats:p> Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium <jats:named-content content-type="genus-species">Aphanothece halophytica</jats:named-content> , which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes. <jats:italic>In vivo</jats:italic> analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The <jats:named-content content-type="genus-species">Aphanothece</jats:named-content> MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed <jats:named-content content-type="genus-species">Escherichia coli</jats:named-content> and <jats:named-content content-type="genus-species">Synechococcus</jats:named-content> strains expressing four putative <jats:named-content content-type="genus-species">Aphanothece</jats:named-content> MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed <jats:named-content content-type="genus-species">E. coli</jats:named-content> cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source. </jats:p> Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica Applied and Environmental Microbiology |
| spellingShingle | Waditee-Sirisattha, Rungaroon, Kageyama, Hakuto, Sopun, Warangkana, Tanaka, Yoshito, Takabe, Teruhiro, Applied and Environmental Microbiology, Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_full | Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_fullStr | Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_full_unstemmed | Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_short | Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| title_sort | identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium aphanothece halophytica |
| title_unstemmed | Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.03729-13 |